RESUMO
In a series of five 17-d replicate trials, a total of 54 cannulated and 12 noncannulated pigs were used to determine the effects of weaning age (17 d or 24 d) on pH, dry matter percentage, aerobic and anaerobic microflora, lactate, and volatile fatty acid (VFA) concentrations in the jejunum, ileum, and cecum of weanling pigs. At -14 d of age, cannulated pigs were surgically fitted with T-cannulas in the jejunum (n = 20), ileum (n = 18), or cecum (n = 16). Upon weaning, cannulated pigs were individually caged in an environmentally controlled room with ad libitum access to a phase starter diet and water. Noncannulated pigs were killed at weaning and samples were collected from the jejunum, ileum, and cecum. Digesta and fecal swabs from cannulated pigs were collected twice weekly. The pH of cecal contents was lower (P < 0.05) and dry matter percentage was greater (P < 0.05) than those ofjejunal or ileal contents. Pigs weaned at 24 d of age had increased (P < 0.05) E. coli populations 3 d postweaning compared to preweaning populations, regardless of site of collection, whereas this increase was not observed in pigs weaned at 17 d of age. Unweaned pigs maintained higher (P < 0.05) lactobacilli populations compared to weaned pigs; however, populations declined (P < 0.05) in both groups by 3 d postweaning, with pigs weaned at 24 d of age having lactobacilli populations greater than pigs weaned at 17 d of age. Fecal populations of E. coli and lactobacilli declined (P < 0.05), whereas fecal bifidobacteria populations increased (P < 0.05) postweaning, regardless of weaning age. Concentrations of total fecal anaerobes declined (P < 0.05) in pigs weaned at 17 d of age but were maintained in pigs weaned at 24 d of age. Volatile fatty acid concentrations were greater (P < 0.05) in the cecum than in the jejunum or ileum, and acetic acid concentrations decreased (P < 0.05) postweaning regardless of weaning age. A tendency for L+ lactate concentrations to be greater (P < 0.07) in the ileum and jejunum vs the cecum was observed. Results indicate that weaning and weaning age have significant effects on microbial populations and VFA concentrations.
Assuntos
Ácidos Graxos Voláteis/análise , Mucosa Intestinal/metabolismo , Intestinos/microbiologia , Suínos/fisiologia , Desmame , Fatores Etários , Ração Animal , Animais , Bactérias Anaeróbias/crescimento & desenvolvimento , Bactérias Anaeróbias/isolamento & purificação , Ceco/metabolismo , Ceco/microbiologia , Contagem de Colônia Microbiana/veterinária , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/isolamento & purificação , Fezes/química , Fezes/microbiologia , Feminino , Conteúdo Gastrointestinal/química , Conteúdo Gastrointestinal/microbiologia , Hidrocortisona/sangue , Concentração de Íons de Hidrogênio , Íleo/metabolismo , Íleo/microbiologia , Jejuno/metabolismo , Jejuno/microbiologia , Lactatos/metabolismo , Lactobacillaceae/crescimento & desenvolvimento , Lactobacillaceae/isolamento & purificação , Masculino , Suínos/microbiologiaRESUMO
New compounds bastadin 20 (9), 15,34-O-disulfatobastadin 7 (10), and 10-O-sulfatobastadin 3 (11) were isolated from Ianthella basta collected in Exmouth Gulf, Western Australia. Compounds 10 and 11 exhibited moderate differential activity as SR Ca2+ channel agonists (EC50 13.6 and 100 microM, respectively) of the Ry1R FKBP12 complex, while the potency of 9 was almost half that of 10 (EC50 20.6 microM). The problem of dereplication of bastadins was addressed using 1H-NMR "fingerprinting" of MeO signals in the corresponding permethyl bastadin derivatives.
Assuntos
Agonistas dos Canais de Cálcio/química , Canais de Cálcio/metabolismo , Proteínas Musculares/metabolismo , Peptídeos Cíclicos/química , Éteres Fenílicos/química , Poríferos/química , Animais , Agonistas dos Canais de Cálcio/farmacologia , Canais de Cálcio/efeitos dos fármacos , Linhagem Celular , Éteres Difenil Halogenados , Espectroscopia de Ressonância Magnética , Proteínas Musculares/efeitos dos fármacos , Peptídeos Cíclicos/farmacologia , Éteres Fenílicos/farmacologia , Canal de Liberação de Cálcio do Receptor de Rianodina , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por MatrizRESUMO
The objective of this study was to develop a polymerase chain reaction (PCR)-based method to detect and differentiate among Escherichia coli strains containing genes for the expression of 3 antigenic variants of the fimbrial adhesin K88 (K88ab, K88ac, and K88ad). Five primers were designed that allowed detection of K88+ E. coli, regardless of antigenic variant, and the separate detection of the ab, ac, and ad variants. Primers AM005 and AM006 are 21 base pair (bp) oligomers that correspond to a region of the K88 operon that is common to all 3 antigenic variants. Primers MF007, MF008, and MF009 are 24-bp oligomers that matched variable regions specific to ab, ac, and ad, respectively. Using primers AM005 and AM006, a PCR product was obtained that corresponds to a 764-bp region within the large structural subunit of the K88 operon common to all 3 antigenic variants. Primer AM005 used with MF007, MF008, or MF009 produced PCR products approximately 500-bp in length from within the large structural subunit of the K88 operon of the 3 respective antigenic variants. Fragments were identified by rates of migration on a 1% agarose gel relative to each other as well as to BstEII-digested lambda fragments. This PCR-based method was comparable to the enzyme-linked immunosorbent assay and western blot test in the ability to differentiate between the antigenic variants. K88+ E. coli were differentiated from among laboratory strains and detected in ileal samples taken from cannulated pigs challenged with a known K88+ variant. K88+ E. coli were also detected from fecal swabs taken from newly weaned pigs, thus confirming that this PCR-based test could provide a convenient clinical assay for the detection of K88+ E. coli. Detection and differentiation of K88+ E. coli using general and specific primers was successful. PCR methods of detection should permit identification of K88+ antigenic variants regardless of the level of expression of the antigen.
Assuntos
Adesinas de Escherichia coli/genética , Escherichia coli/classificação , Escherichia coli/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Animais , Aderência Bacteriana , Western Blotting/métodos , Primers do DNA , Diarreia/microbiologia , Diarreia/veterinária , Ensaio de Imunoadsorção Enzimática/métodos , Escherichia coli/genética , Infecções por Escherichia coli/diagnóstico , Infecções por Escherichia coli/veterinária , Variação Genética , Plasmídeos , Suínos , Doenças dos SuínosRESUMO
Systematic, through testing of decision support systems (DSSs) prior to release to general users is a critical aspect of high quality software design. Omission of this step may lead to the dangerous, and potentially fatal, condition of relying on a system with outputs of uncertain quality. Thorough testing requires a great deal of effort and is a difficult job because tools necessary to facilitate testing are not well developed. Testing is a job ill-suited to humans because it requires tireless attention to a large number of details. For these reasons, the majority of DSSs available are probably not well tested prior to release. We have successfully implemented a software design and testing plan which has helped us meet our goal of continuously improving the quality of our DSS software prior to release. While requiring large amounts of effort, we feel that the process of documenting and standardizing our testing methods are important steps toward meeting recognized national and international quality standards. Our testing methodology includes both functional and structural testing and requires input from all levels of development. Our system does not focus solely on meeting design requirements but also addresses the robustness of the system and the completeness of testing.
Assuntos
Técnicas de Apoio para a Decisão , Terapia Assistida por Computador , Inteligência Artificial , Reprodutibilidade dos Testes , Respiração Artificial , Design de SoftwareRESUMO
The exact masses of bastadins, cyclic peptides from marine sponges Ianthella basta, are determined using matrix-assisted laser desorption ionization (MALDI) coupled to a Fourier transform mass spectrometer. Two known compounds were mixed with the unknown to serve as internal calibrants. The mass of the calibrants bracketed the mass of the unknown compound. With this method, exact masses were obtained to within 5 ppm for single determinations, and less than 3 ppm for multiple determinations, allowing the derivation of elemental composition. This method is viable for routinely obtaining the exact masses of new compounds with MALDI.
Assuntos
Peptídeos Cíclicos/análise , Poríferos/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Animais , Calibragem , Análise de Fourier , Peso Molecular , Peptídeos Cíclicos/química , Padrões de ReferênciaRESUMO
There are often clear lines drawn identifying the demilitarized zone between medical informatics academics and industry. Academics were "pure" intellectuals sequestered in ivory towers that effectively shielded them from the realities of the world. Industry has historically focused on creating effective products that produce financial return to the corporation. Both the paradigms of academia and industry are quickly becoming dinosaurs in the era of health care reform where both medical informatics academia and industry are under increasing pressure to develop and prove that medical informatics has a positive impact on health care both in terms of the quality of care as well as cost. Unfortunately, neither academia or industry alone are going to be able to successfully complete this task. The purpose of this paper is to describe such a collaborative effort that has produced a computerized decision support system for the management of mechanical ventilation in patients with the Adult Respiratory Distress Syndrome (ARDS) that is now installed and supported on three different commercial CIS platforms. This collaborative effort has allowed us to successfully mount a large multi-center clinical trial designed to determine efficacy.
Assuntos
Comportamento Cooperativo , Sistemas de Apoio a Decisões Administrativas , Respiração Artificial , Síndrome do Desconforto Respiratório/terapia , Protocolos Clínicos/normas , Tomada de Decisões Assistida por Computador , Difusão de Inovações , Reforma dos Serviços de Saúde , Sistemas de Informação Hospitalar , Humanos , Indústrias , Informática Médica , Estudos Prospectivos , Respiração Artificial/normas , Estados Unidos , UniversidadesRESUMO
Pigs cannulated at the ileum were used to investigate the effect of creep feeding on specific intestinal microflora and VFA and lactate concentrations. In two replicate trials performed 50 d apart, a total of 24 15-d-old nursing pigs from seven sows were cannulated in the terminal ileum and randomly assigned to two test groups. One group was allowed access to a creep diet, whereas the other group was denied access to the diet by removal from the farrowing crate. Ileal samples were collected at 19, 22, 26, and 29 d of age (preweaning) and at 33, 36, and 40 d of age (postweaning). Samples were assayed for total and hemolytic E. coli, total lactobacilli, pH, acetate, propionate, butyrate, isobutyrate, valerate, isovalerate, D(-), and L(+) lactate. Creep feeding did not affect lactobacilli or E. coli concentrations (P > .05), nor did it affect short-chain fatty acid concentrations (P > .05). Most volatile fatty acid concentrations decreased (P < .05), total lactate increased (P < .05), and the proportion of E. coli that were hemolytic increased (P < .05) following weaning. Consumption of creep feed did not affect specific microbial populations or fermentative byproducts in the ileum of the young pig.
Assuntos
Ração Animal/normas , Ácidos Graxos Voláteis/metabolismo , Íleo/metabolismo , Íleo/microbiologia , Suínos/metabolismo , Ração Animal/análise , Animais , Cruzamentos Genéticos , Escherichia coli/isolamento & purificação , Escherichia coli/fisiologia , Ácidos Graxos Voláteis/análise , Feminino , Concentração de Íons de Hidrogênio , Íleo/química , Lactatos/análise , Lactatos/metabolismo , Lactobacillaceae/isolamento & purificação , Lactobacillaceae/fisiologia , Masculino , Distribuição Aleatória , Glycine max/normas , Desmame , Zea mays/normasAssuntos
Atitude Frente a Morte , Casas de Saúde/organização & administração , Adaptação Psicológica , Adulto , Ansiedade/prevenção & controle , Feminino , Administradores de Instituições de Saúde/estatística & dados numéricos , Humanos , Liderança , Masculino , Casas de Saúde/estatística & dados numéricos , Relações Profissional-Família , Inquéritos e Questionários , TexasRESUMO
Forty-eight continuous culture enrichments were performed on summer and winter water samples from two contrasting rivers. The cell output from each chemostat was dependent on the temperature and nitrogen concentration of each enrichment. The diversity of the populations from the continuous cultures, as assessed by species diversity analysis, was always greater than populations obtained on agar plates. However, the species isolated exclusively by continuous culture in these experiments were not unique to the chemostat. All of these species had been isolated at some time on plates directly. High nitrogen concentrations were found to decrease diversity. Populations sampled during the winter were influenced primarily by the concentration of nitrogen. Summer isolates were affected mainly by the temperature of the culture medium. The nutritional versatility of the population was not affected by enrichment of the continuous culture.
Assuntos
Bactérias/isolamento & purificação , Estações do Ano , Microbiologia da Água , Análise de Variância , Bactérias/classificação , Bactérias/metabolismo , Canadá , Água Doce , Nitrogênio/metabolismo , TemperaturaRESUMO
The heterotrophic bacterial populations in two contrasting rivers have been examined over a period of 1 year. The populations were analyzed (i) as total heterotrophic counts, (ii) as species numbers, using numerical taxonomy, (iii) by diversity indices, and (iv) by factor analysis. Isolates were obtained by plating directly from water samples and by chemostat enrichment. Four factors emerged which profiled the bacterial community and were common to both rivers. They were, in order of decreasing importance, fermentative metabolism, inorganic nitrogen metabolism, fluorescence-oxidative metabolism, and lack of starch hydrolysis. Several factors produced significant correlations with a range of physicochemical parameters, which were also measured. The correlations suggested an intricate algal-bacterial interaction. The oxidative metabolism factor correlated with rainfall in one river, suggesting that the oxidative bacteria may be washed in from the surrounding land. In the other river, the oxidative-fermentative factor correlated negatively with sunshine. Factor analysis was the most effective method for revealing correlations between bacterial characteristics and the environmental parameters; however, the use of a variety of methods provided more insight into the ecological aspects.
Assuntos
Transtornos de Enxaqueca/terapia , Adolescente , Adulto , Criança , Eletromiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Temperatura CutâneaRESUMO
Isophthalic acid, 5-carboxy-, 5-hydroxy-, 5-methoxy-, 5-fluoro-, 5-bromo-, 5-cyano-, and 5-methylisophthalic acid were inhibitors competitive with L-glutamate for bovine liver glutamate dehydrogenase. The extent of inhibition by the derived compounds was not much greater than that obtained with the parent compound, isophthalic acid. A plot of pKi versus pH showed the presence of an ionizable group (pKa 7.4-7.8) at the enzyme active site which interacted with the substitutent at the 5 position of the substituted isophthalates.
Assuntos
Glutamato Desidrogenase/antagonistas & inibidores , Fígado/enzimologia , Ácidos Ftálicos/farmacologia , Animais , Ligação Competitiva , Bovinos , Concentração de Íons de Hidrogênio , Ácidos Ftálicos/síntese química , Relação Estrutura-AtividadeRESUMO
The present report deals with the survival of micro-organisms in the gastric lumen of fasting human subjects. Studies were carried out on hospitalized adult patients during the day, at night and over a 24-hour period.It is concluded that the survival of bacteria in the gastric lumen depends on the pH of the gastric juice. The duration of survival of the organisms at the pH <3.0 varied; however, at pH> 4.0, when a subsequent rapid drop in pH occurred, the clearing of viable organisms occurred within the hour sampling period in most cases. It is possible that medications which decrease gastric acidity favour an increase in the growth of gastric bacteria, while substances with a low pH may decrease bacterial growth.
Assuntos
Bactérias , Jejum , Suco Gástrico , Concentração de Íons de Hidrogênio , Estômago , Adulto , HumanosRESUMO
Fluorescein-labeled immunoglobulin G (IgG) fractions of serum from patients with acute poststreptococcal glomerulonephritis stained parts of the glomerular basement membrane and mesangium of kidney tissue obtained from the same patients during the early phase of the disease. Renal tissue obtained from normal individuals and from patients with other kidney diseases failed to stain with these IgG fractions. Preabsorption of the serum fractions with various freezethawed bacteria demonstrated that only certain group A streptococci abolished the staining capacity. Fractionation of the streptococci into cellular constituents indicated that it was predominantly the plasma membrane fraction which blocked the immune staining. Spectrofluorometry using alkali-solubilized renal tissue confirmed these observations in a quantitative manner. By sucrose density-gradient ultracentrifugation of the plasma membrane two possible antigens were isolated. One was soluble in phosphate-buffered saline and the other was insoluble. The soluble component was a lipoprotein with a molecular weight of approximately 120,000.
Assuntos
Membrana Celular/imunologia , Glomerulonefrite/imunologia , Imunoglobulina G/análise , Adulto , Antígenos/isolamento & purificação , Membrana Basal/imunologia , Biópsia , Centrifugação com Gradiente de Concentração , Criança , Fluoresceínas , Fluorometria , Humanos , Glomérulos Renais/imunologia , Lipoproteínas/análise , Análise Espectral , Coloração e Rotulagem , Streptococcus/imunologiaRESUMO
The presence and survival of ingested micro-organisms, both pathogenic and commensal, is of practical interest because the stomach is one of the principal portals of entry of bacteria in humans. A study of the environmental factors governing the survival of indigenous flora in the gastric lumen will assist in determining whether potentially pathogenic organisms will survive in the stomach and pass on. The authors set out to determine the significance of gastric mucin in such survival, with the ultimate objective of learning what factors provide optimal bactericidal conditions.Samples of gastric juice were aspirated from 149 fasting individuals; samples of saliva, nose and throat swabs were also obtained. Positive gastric cultures were found in 82%. The study of the effects of gastric pH demonstrated an increase in the number of samples showing growth above pH 2.0 and a concomitant increase in total bacterial growth. At a pH lower than 2.0, certain organisms are selected out. It appears that even minor changes in gastrointestinal physiology disrupt the normal clearing of the flora.