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1.
J Med Case Rep ; 3: 7314, 2009 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-19830180

RESUMO

INTRODUCTION: Epstein-Barr virus esophagitis in an immunocompetent host is a rare entity. It represents either primary infection or reactivation and is usually characterized by acute onset and extensive ulcerative involvement of the upper and middle third of the esophagus. CASE PRESENTATION: A case of Epstein-Barr virus esophagitis in a 27-year-old woman with no immunosuppressive factors, and having gastrointestinal symptoms is reported here. Using real-time polymerase chain reaction, biopsy and blood specimens were tested for candida and herpes viruses. Epstein-Barr virus DNA was detected in tissue samples. The patient was treated with acyclovir with resolution of the symptomatology. CONCLUSIONS: The prevalence of esophagitis remains undefined in both immunodeficient and immunocompetent individuals and should be taken into consideration in a patient presenting with esophageal symptoms. This case report stresses the role of Epstein-Barr virus infection in the pathogenesis of esophagitis, a rare condition in an immunocompetent host. In this setting, active infection may represent a primary infection or reactivation. Histopathological examination alone may miss the diagnosis, while polymerase chain reaction techniques optimize the diagnostic sensitivity, establish a diagnosis, and lead to an appropriate therapy.

3.
J Clin Virol ; 40(1): 77-9, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17662647

RESUMO

BACKGROUND: Enteroviruses are the most common cause of aseptic meningitis, presenting in epidemic or endemic form. OBJECTIVES: To determine the causative agent of an aseptic meningitis outbreak in autumn, 2005 in Patras, Greece. STUDY DESIGN: Cerebrospinal fluid (CSF) samples taken during May 2005-February 2006 from children admitted to the Children Hospital of Patras with signs of aseptic meningitis were tested for the presence of enteroviral RNA. Typing was performed by nucleotide analysis. RESULTS: Enteroviruses were detected in 11 (57.9%) of 19 tested CSF samples. In a 12-day period (27 October-7 November 2005) five aseptic meningitis cases were observed. Echovirus 15 was detected in all five cases, and differed from the prototype strain by 27.6%. Enteroviruses before and after this cluster of cases were of different serotypes (Echovirus 9, Echovirus 6). All patients with Echovirus 15 infection were male with a mean age of 7.7 years (2 months-13 years), all recovered successfully. CONCLUSIONS: This is the first report of a cluster of aseptic meningitis cases caused by Echovirus 15. The causative agent was a new variant of Echovirus 15.


Assuntos
Surtos de Doenças , Infecções por Echovirus/epidemiologia , Enterovirus Humano B/isolamento & purificação , Meningite Asséptica/epidemiologia , Adolescente , Criança , Pré-Escolar , Infecções por Echovirus/líquido cefalorraquidiano , Infecções por Echovirus/virologia , Grécia/epidemiologia , Humanos , Lactente , Masculino , Meningite Asséptica/líquido cefalorraquidiano , Meningite Asséptica/virologia
4.
J Clin Lab Anal ; 20(5): 177-83, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16960900

RESUMO

Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated for EVs using viral culture and/or molecular methods directly in the cerebrospinal fluid (CSF). EVs were detected in 21 out of 52 (40.4%) patients using reverse transcription-PCR (RT-PCR) and/or tissue culture. EVs were isolated from six out of 37 (16.2%) cultured specimens, while 20 out of 52 (38.4%) specimens yielded positive results when 5'non-coding region (5'NCR) RT-PCR assay was used. One specimen that was culture-positive was RT-PCR-negative. Using the VP1-2A RT-PCR and sequence analysis, 14 of the 21 positive EVs were identified as: four strains of Coxsackie virus B5, five echovirus 11, two echovirus 9, one echovirus 5, one echovirus 14, and one Coxsackie virus A9. Fever, headache, vomiting, and stiff neck were the most pronounced symptoms. Pleocytosis with the predominance of lymphocytes and mild elevated protein levels characterized the CSF specimens. Coxsackie virus B5 and echovirus 11 were the predominant serotypes during the study period. Although there was seasonal enteroviral activity (April-November), cases also occurred in the cold months. The 5'NCR and VP1-2A RT-PCR with sequence analysis were found to be superior to conventional methods for direct diagnosis and the typing of EVs.


Assuntos
Infecções por Enterovirus/líquido cefalorraquidiano , Infecções por Enterovirus/diagnóstico , Meningite Viral/líquido cefalorraquidiano , Meningite Viral/diagnóstico , Adolescente , Adulto , Idoso , Células Cultivadas , Criança , Pré-Escolar , Enterovirus/classificação , Enterovirus/genética , Enterovirus/isolamento & purificação , Infecções por Enterovirus/fisiopatologia , Grécia , Humanos , Lactente , Recém-Nascido , Meningite Viral/fisiopatologia , Pessoa de Meia-Idade , RNA Viral/análise , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sorotipagem
5.
J Med Virol ; 75(3): 440-6, 2005 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-15648067

RESUMO

Thirty strains of adenovirus (Ads) associated with ocular disease have been isolated over a period of 4 years in Thessaloniki, Northern Greece. Eleven strains were isolated from sporadic patients with conjunctivitis or keratoconjunctivitis in Thessaloniki city between 1998 and 2000. Nineteen strains were isolated from patients with keratoconjunctivitis during an outbreak of Ads in the area of Thessaloniki (Thessaloniki and Serres cities) in 2002. PCR-sequence method using primers targeted against the hypervariable regions (HVRs) of hexon gene, as well as the neutralization test were used for typing the Ad isolates and assessing a possible relation among these strains, and their genetic variability. Ad4 with very close homology to variant Z-G 95-873 was the most frequent genotype causing sporadic conjunctivitis over a period of 4 years. Two other strains, one Ad2, and one Ad3 were similar to the prototype ones, and a third one shows close homology to the variant of prototype Ad15, the Morrison strain. The genome typing of twenty two Ad8 isolates showed very close homology in their amino acid and nucleotide sequences to the variant of Ad8, strain 1127 (accession no. X74663). Four were isolated from patients with keratoconjunctivitis in 1998, 1999, 2000 and 18 during the outbreak in 2002. As far as strain 1127 is concerned, all the Ad8 isolates showed the same changes in the HVR 1 and HVR 2 except one isolate in 1998, which showed some changes outside the HVRs. During the outbreak of Ad8 keratoconjunctivitis, it was not possible to identify the exact source of infection (nosocomial or/and outpatients). Finally, Ad4 variant Z-G 95-873 and Ad8 which is closely related to the strain 1127, were found to be the predominant adenoviruses circulating in Northern Greece during 1998-2002.


Assuntos
Infecções por Adenoviridae/virologia , Adenoviridae/genética , Conjuntivite/virologia , Ceratoconjuntivite/virologia , Epidemiologia Molecular , Adenoviridae/classificação , Adenoviridae/imunologia , Adenoviridae/isolamento & purificação , Infecções por Adenoviridae/epidemiologia , Sequência de Aminoácidos , Proteínas do Capsídeo/genética , Regiões Determinantes de Complementaridade/genética , Conjuntivite/epidemiologia , DNA Viral/química , DNA Viral/isolamento & purificação , Genótipo , Grécia/epidemiologia , Humanos , Ceratoconjuntivite/epidemiologia , Dados de Sequência Molecular , Testes de Neutralização , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos
6.
J Clin Lab Anal ; 18(6): 296-8, 2004.
Artigo em Inglês | MEDLINE | ID: mdl-15543564

RESUMO

Here we report the results of a retrospective study on the epidemiological characteristics and genetic relationships of the virus isolates responsible for the last poliomyelitis cases in Greece. The last wild poliomyelitis case in Greece was detected in 1996, and the last vaccine-related strain was isolated in 1998. The whole of Europe, including Greece, is now considered to be polio-free.


Assuntos
Poliomielite/epidemiologia , Poliovirus/isolamento & purificação , Grécia , Humanos , Filogenia , Poliomielite/virologia , Poliovirus/classificação , Poliovirus/genética , Vacinas contra Poliovirus/genética , Estudos Retrospectivos
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