In vitro glucuronidation of the antibacterial triclocarban and its oxidative metabolites.

Triclocarban (3,4,4'-trichlorocarbanilide; TCC) is widely used as an antibacterial in bar soaps. During use of these soaps, a significant portion of TCC is absorbed by humans. For the elimination from the body, glucuronidation plays a key role in both biliary and renal clearance. To investigate this metabolic pathway, we performed microsomal incubations of TCC and its hydroxylated metabolites 2'-OH-TCC, 3'-OH-TCC, and 6-OH-TCC. Using a new liquid chromatography-UV-mass spectrometry method, we could show a rapid glucuronidation for all OH-TCCs by the uridine-5'-diphosphate-glucuronosyltransferases (UGT) present in liver microsomes of humans (HLM), cynomolgus monkeys (CLM), rats (RLM), and mice (MLM). Among the tested human UGT isoforms, UGT1A7, UGT1A8, and UGT1A9 showed the highest activity for the conjugation of hydroxylated TCC metabolites followed by UGT1A1, UGT1A3, and UGT1A10. Due to this broad pattern of active UGTs, OH-TCCs can be efficiently glucuronidated in various tissues, as shown for microsomes from human kidney (HKM) and intestine (HIM). The major renal metabolites in humans, TCC-N-glucuronide and TCC-N'-glucuronide, were formed at very low conversion rates (<1%) by microsomal incubations. Low amounts of N-glucuronides were generated by HLM, HIM, and HKM, as well as by MLM and CLM, but not by RLM, according to the observed species specificity of this metabolic pathway. Among the human UGT isoforms, only UGT1A9 had activity for the N-glucuronidation of TCC. These results present an anomaly where in vivo the predominant urinary metabolites of TCC are N and N'-glucuronides, but these compounds are slowly produced in vitro.

Speckle interferometry with temporal phase evaluation for measuring large-object deformation.

We propose a new method for measuring large-object deformations byusing temporal evolution of the speckles in speckleinterferometry. The principle of the method is that by deformingthe object continuously, one obtains fluctuations in the intensity ofthe speckle. A large number of frames of the object motion arecollected to be analyzed later. The phase data for whole-objectdeformation are then retrieved by inverse Fourier transformation of afiltered spectrum obtained by Fourier transformation of thesignal. With this method one is capable of measuring deformationsof more than 100 mum, which is not possible using conventionalelectronic speckle pattern interferometry. We discuss theunderlying principle of the method and the results of theexperiments. Some nondestructive testing results are alsopresented.

Shape Measurement by use of Temporal Fourier transformation in Dual-Beam Illumination Speckle Interferometry.

We outline a novel method for determining the shape of an object by use of temporal Fourier-transform analysis in dual-beam illumination speckle interferometry. The object whose shape is to be determined is rotated about an axis, and a number of frames of the image of the object motion are acquired. Temporal in-plane displacement that is due to the object rotation is related to the shape of the object and is retrieved from this large set of data by Fourier transformation. With this method one can determine the absolute height of the object with variable resolution, thereby allowing shapes of objects with large and small slopes to be determined. The theory of the method along with experimental results is presented.

Oblique incidence and observation electronic speckle-pattern interferometry.

In this paper we discuss an oblique incidence and observation electronic speckle-pattern interferometer, in which we use an anamorphic prism in front of the object. A collimated beam traveling through the prism is partly reflected at the base of the prism. The reflected light is the reference beam and the transmitted light illuminates the diffuse object, thereby generating the object beam. In this scheme the object and the reference beams are collinear. A new scheme that uses two prisms and permits phase stepping is also presented.