RESUMO
Seven children who presented during the influenza A(H1N1) epidemic of 1988 are described. After a typical influenzal illness, they developed haematemesis of varying severity. Endoscopy revealed haemorrhagic gastritis. Laboratory evidence of influenza A(H1N1) virus infection was present. Two children died as a result of their illness. The association of virus and gastrointestinal haemorrhage is explored.
Assuntos
Surtos de Doenças , Hematemese/etiologia , Vírus da Influenza A/isolamento & purificação , Influenza Humana/complicações , Criança , Pré-Escolar , Doenças em Gêmeos , Feminino , Gastrite/etiologia , Hematemese/epidemiologia , Hematemese/microbiologia , Humanos , Vírus da Influenza A/classificação , Influenza Humana/epidemiologia , Influenza Humana/microbiologia , Masculino , Nasofaringe/microbiologia , Queensland/epidemiologia , Conglomerados Espaço-TemporaisRESUMO
An infant with a deficiency of the enzyme uridine diphosphate galactose-4-epimerase was detected during galactosaemia screening of the Queensland newborn population. No case of epimerase deficiency has been reported previously in Australia and the incidence in our population is unknown. A deficiency of this enzyme is usually quite benign although two cases with a galactosaemia-like syndrome have been reported. This infant is developing normally, both intellectually and physically, in spite of extremely high levels of red blood cell galactose-1-phosphate. The introduction of newer methods of galactosaemia screening in Australia will probably result in the detection of other cases of this enzyme deficiency.
Assuntos
Carboidratos Epimerases/deficiência , Galactosemias/prevenção & controle , Programas de Rastreamento , UDPglucose 4-Epimerase/deficiência , Austrália , Serviços de Saúde da Criança , Galactosemias/epidemiologia , Humanos , Recém-Nascido , MasculinoAssuntos
Bacillus cereus , Genética Microbiana , Heterozigoto , Bacillus cereus/crescimento & desenvolvimento , Bacillus cereus/metabolismo , Mapeamento Cromossômico , Cruzamentos Genéticos , Meios de Cultura , Mutagênicos , Nitrosoguanidinas , Recombinação Genética , Espectrofotometria , Esporos Bacterianos/crescimento & desenvolvimentoAssuntos
Amidoidrolases , Glicerofosfatos , Pentosefosfatos , Acrilatos , Amidoidrolases/análise , Amidoidrolases/isolamento & purificação , Bacillus subtilis/enzimologia , Isótopos de Carbono , Parede Celular/enzimologia , Fenômenos Químicos , Precipitação Química , Química , Cromatografia em Gel , Cromatografia por Troca Iônica , Eletroforese , Etanol , Géis , Temperatura Alta , Métodos , Microquímica , Polissacarídeos , Especificidade da Espécie , EspectrofotometriaAssuntos
Bacillus cereus , Bacteriófagos , DNA Viral/metabolismo , Streptococcus , Transformação Genética , Bacteriófagos/crescimento & desenvolvimento , Centrifugação com Gradiente de Concentração , DNA Bacteriano/metabolismo , Genética Microbiana , Isótopos de Fósforo , Timina/metabolismo , TrítioRESUMO
Cells of Escherichia coli were labeled with precursors of ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and protein, lysed with detergent, and examined by starch-block electrophoresis and CsCl density gradient centrifugation. A large amount of the DNA was seen to remain at positions of low electrophoretic mobility and light density along with tryptophan and arginine-containing proteins and some RNA. Addition of labeled, phenol-extracted DNA to unlabeled cells prior to lysis and electrophoresis showed that only a small amount of the DNA became associated during or after lysis. Sonic treatment of a lysate removed most of the DNA to a position of electrophoretic mobility and density similar to that of free DNA, whereas pronase and ribonuclease released only a part of the DNA. We concluded that binding of DNA to cell membranes or other cell components occurs in the cell prior to lysis and involves protein and probably a specific type of RNA.
