RESUMO
This study investigated the relationship between biological and psychological processes in the recovery phase of depression during treatment with cognitive therapy. In particular, we tested the hypothesis that biological and psychological processes are independent; the hypothesis that they are related in a linear or sequential process; and the hypothesis that there is a circular relationship between them. 35 depressed patients completed a 12 week program of cognitive therapy. Changes in measures of negative thinking and amine dysfunction over the course of therapy were compared for those who improved and those who did not. There was a significant relationship between improvement in symptoms and change for ATQ scores, DAS scores, and epinephrine levels. There was significant change in metanephrine levels during therapy which was not related to improvement. The changes were not consistent across indices. Although the results did not provide definite support for any of the four models of the relationship between biological and psychological variables during recovery from depression, the finding that there was some change in some of the indices of amine dysfunction during cognitive therapy indicates that the interface between biological and psychological processes in depression should continue to be studied.
Assuntos
Transtornos de Adaptação/terapia , Terapia Cognitivo-Comportamental , Transtorno Depressivo Maior/terapia , Transtorno Distímico/terapia , Epinefrina/urina , Metanefrina/urina , Adaptação Psicológica/fisiologia , Transtornos de Adaptação/fisiopatologia , Transtornos de Adaptação/psicologia , Adulto , Idoso , Encéfalo/fisiopatologia , Transtorno Depressivo Maior/fisiopatologia , Transtorno Depressivo Maior/psicologia , Transtorno Distímico/fisiopatologia , Transtorno Distímico/psicologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Norepinefrina/urina , Normetanefrina/urina , Pensamento/fisiologia , Resultado do TratamentoRESUMO
Psychrotrophic lipolytic bacteria represent a significant problem in the storage of refrigerated dairy products. A lipase-encoding gene has been cloned and characterized from a highly lipolytic strain of Pseudomonas. The nucleotide sequence of the gene predicts a polypeptide of M(r) 49,905, which was identified when the gene was expressed in Escherichia coli.
Assuntos
Escherichia coli/genética , Lipase/genética , Leite/microbiologia , Pseudomonas/metabolismo , Triglicerídeos/metabolismo , Sequência de Aminoácidos , Animais , Proteínas de Bactérias/genética , Proteínas de Bactérias/isolamento & purificação , Sequência de Bases , Biodegradação Ambiental , Expressão Gênica , Genes Bacterianos , Vetores Genéticos , Cabras , Lipase/metabolismo , Dados de Sequência Molecular , Pseudomonas/enzimologia , Pseudomonas/genética , Pseudomonas/isolamento & purificaçãoRESUMO
A 12-session group program of cognitive therapy, designed by the first author, was evaluated with 35 persons suffering from major or minor depressive disorders. Effect-size scores were generated using the method of Nietzel, Russel, Hemmings, and Gretter (1987) and compared with cut-off points calculated using the method of Jacobson and Revenstorf (1988) and the norms established by Nietzel et al. (1987) in their meta-analysis. The effects of the Group Cognitive Therapy Program were found to be clinically significant according to the criteria of Jacobson and colleagues (Jacobson and Revenstorf, 1988; Jacobson, Follette and Revenstorf, 1984) for 73% of patients. It is concluded that the Group Cognitive Therapy Program is a clinically efficacious and cost-effective treatment for persons suffering from nonpsychotic, primary, unipolar depression.
Assuntos
Terapia Cognitivo-Comportamental/métodos , Transtorno Depressivo/psicologia , Transtorno Depressivo/terapia , Psicoterapia de Grupo/métodos , Seguimentos , Humanos , Apego ao Objeto , Autoimagem , Transferência PsicológicaRESUMO
A plasmid, pRG1, has been constructed by incorporating the coding sequence of human hypoxanthine-guanine phosphoribosyltransferase (HPRT) into the expression vector pT7-7. Expression of human HPRT has been achieved in HPRT- Escherichia coli cells transformed with pRG1 and pGP1-2, as shown by: (1) exclusive labelling with [35S]methionine of a polypeptide with the same mobility as purified human HPRT on SDS-PAGE; and (2) measurement of HPRT activity after cell lysis. Although the majority of the recombinant HPRT was present in the particulate fraction after cell lysis and centrifugation, sufficient HPRT activity was present in the supernatant fraction to allow comparison with the HPRT purified from human erythrocytes and the activity in human haemolysates and lymphoblast lysates. Small differences in electrophoretic mobility on native gels were found between HPRT activity from these sources. The Km values of recombinant HPRT for the substrates 5-phospho-alpha-D-ribosyl-1-pyrophosphate and guanine were compared with those of lymphoblast and erythrocyte HPRT.
Assuntos
Escherichia coli/genética , Expressão Gênica , Hipoxantina Fosforribosiltransferase/genética , Cromatografia em Gel , Clonagem Molecular , DNA/genética , Eletroforese em Gel de Poliacrilamida , Éxons , Genes , Humanos , Hipoxantina Fosforribosiltransferase/química , Hipoxantina Fosforribosiltransferase/metabolismo , Cinética , Metionina/farmacologia , Plasmídeos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Transformação BacterianaRESUMO
Samples of 12 hospitalized Korsakoffs, 12 hospitalized non-Korsakoff alcoholics, and 12 controls, all groups being of comparable age range, were tested using a set of seven visual and olfactory measures which involved memory. By stepwise multiple discriminant analysis it was shown that strong separation could be effected between the three groups, solely on the basis of the olfactory recognition and recall tests, which included the Campbell-Gregson (1972) procedure. The use of of anosmia as a diagnostic indicator for Korsakoff's syndrome is discussed.
