1.
Biol Mass Spectrom
; 23(12): 719-26, 1994 Dec.
Artigo
em Inglês
| MEDLINE
| ID: mdl-7841206
RESUMO
A new method was developed for subjecting a peptide to a specified number of Edman degradation cycles on an automated polypeptide sequencer and desorbing the residual peptide for further investigations. The procedure was applied in combination with electrospray ionization mass spectrometry to identify the four disulfide bridges present in a small tightly bound peptide. The task was accomplished using only a few nanomoles of the intact peptide.