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Sci Rep ; 10(1): 12608, 2020 07 28.
Artigo em Inglês | MEDLINE | ID: mdl-32724125

RESUMO

Capsular polysaccharides are important virulence factors in pathogenic bacteria. Characterizing the structural components and biosynthetic pathways for these polysaccharides is key to our ability to design vaccines and other preventative therapies that target encapsulated pathogens. Many gram-negative pathogens such as Neisseria meningitidis and Escherichia coli express acidic capsules. The E. coli K15 serotype has been identified as both an enterotoxigenic and uropathogenic pathogen. Despite its relevance as a disease-causing serotype, the associated capsular polysaccharide remains poorly characterized. We describe in this report the chemical structure of the K15 polysaccharide, based on chemical analysis and nuclear magnetic resonance (NMR) data. The repeating structure of the K15 polysaccharide consists of 4)-α-GlcpNAc-(1 → 5)-α-KDOp-(2 → partially O-acetylated at 3-hydroxyl of GlcNAc. We also report, the organization of the gene cluster responsible for capsule biosynthesis. We identify genes in this cluster that potentially encode an O-acetyltransferase, an N-acetylglucosamine transferase, and a KDO transferase consistent with the structure we report.


Assuntos
Cápsulas Bacterianas/química , Cápsulas Bacterianas/genética , Escherichia coli/genética , Polissacarídeos Bacterianos/química , Polissacarídeos Bacterianos/genética , Espectroscopia de Ressonância Magnética Nuclear de Carbono-13 , Dissacarídeos/química , Família Multigênica , Espectroscopia de Prótons por Ressonância Magnética
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