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6.
Vopr Med Khim ; 22(3): 410-3, 1976.
Artigo em Russo | MEDLINE | ID: mdl-1025898

RESUMO

A modified method is developed for estimation of citric acid in bone tissue. Citrate was isolated from acid bone hydrolysate without a preliminary deproteinization; then it was oxidized and converted into pentabromaceton by treatment of citric acid with bromine. The content of pentabromaceton was estimated by a photometric method. The method was highly sensitive and reproductible.


Assuntos
Osso e Ossos/análise , Citratos/análise , Acetona/análogos & derivados , Acetona/análise , Animais , Osso e Ossos/metabolismo , Citratos/metabolismo , Hidrólise , Técnicas In Vitro , Masculino , Ratos , Espectrofotometria/métodos
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