[Determination of diffusion coefficients in a solution by the transport of a solute through porous membranes]. / Opredelenie koéffitsientov diffuzii v rastvore po perenosu veshchestva cherez poristuiu membranu.

The possibility of determining the coefficients of diffusion in solution by the transport of solutes through porous polymeric membrane was studied. Reliable and reproducible results can be obtained by using nucleoporous filters with cylindrical pores. The method enables the selective determining of the diffusion coefficients of solutes being in complex mixtures, which is of special interest for biochemical research. The possibilities of the method are illustrated on the pattern of some globular proteins, polyethylene glycols and proteolytic enzymes.

[Sedimentation-biochemical method of determination of sedimentation constants and molecular masses of enzymes]. / Sedimentatsionno-biokhimicheskii metod opredeleniia konstant sedimentatsii i molekuliarnykh mass fermentov.

The sedimentation-biochemical method of determination of enzymes' sedimentation coefficients and estimation of their molecular masses based on the Tiselius' transport technique is discussed. The method permits to determine the sedimentation coefficients of enzyme in the starting mixtures without their preliminary purification. The use of different substrats permits to carry out the studying of several enzymes simultaneously. The frameworks of the method are discussed.