The influence on behavior and physiology of white-feathered end-of-cycle hens during simulated transport.

Transportation is a stressful procedure that can alter end-of-cycle hen (EOCH) behavior and physiology. This study (5 × 3 × 2 factorial arrangement) aimed to assess the effects of temperature (T)/relative humidity (RH) (-10°C uncontrolled RH (-10), +21°C 30%RH (21/30), +21°C 80%RH (21/80), +30°C 30%RH (30/30), +30°C 80%RH (30/80)), duration (4, 8, 12 h), and feather cover [well (WF) and poorly-feathered (PF)] on white-feathered EOCH (65-70 wk) behavior and physiology. EOCH (n = 630) from 3 commercial farms were housed for adaptation (3-5 d), fasted (6 h), crated (53 kg/m2), and placed in a climate-controlled chamber. Data collected included chamber and crate conditions, feather condition score, mortality, core body temperature (CBT), behavior, and delta (∆) blood physiology. Analyses were conducted via ANOVA in a randomized complete block design (farm of origin) with significance declared at P ≤ 0.05. PF EOCH had higher mortality than WF hens during cold exposure (-10). EOCH ∆CBT demonstrated a greater (positive) change at 12 h for all T/RH compared to 4 h at 21/30, 21/80, and -10 (negative). Cold exposure (-10) resulted in a higher percentage of time spent shivering and motionless, while heat exposure resulted in a higher percentage of time spent panting for WF EOCH exposed to 30/30 and WF and PF hens exposed to 30/80. Hen ∆glucose had a greater (negative) change at 4 and 12 h for -10 compared to 4 h at 21/30, and all durations for 21/80, 30/30, and 30/80. PF hens exposed to -10 had a greater (positive) change in ∆sodium, ∆hemoglobin, and ∆hematocrit compared to WF birds (negative). The development of metabolic alkalosis was supported by the increase in ∆blood pH over time and the increase in ∆partial pressure of carbon dioxide, ∆bicarbonate, and ∆base excess extracellular fluid during cold exposure (-10). These results indicated that EOCH exposed to heat endured thermal stress while PF hens exposed to cold were unable to cope with cold stress.

The effects of simulated transport on the muscle characteristics of white-feathered end-of-cycle hens.

Transportation of end-of-cycle hens (EOCH) may result in birds' experiencing metabolic stress, which changes muscle characteristics. This study evaluated the impacts of simulated transport on muscle characteristics of white-feathered EOCH (65-70 wk). The factorial arrangement included treatments of T/RH (-10°C uncontrolled RH [-10], 21°C with 30 [21/30] or 80% RH [21/80], 30°C with 30 [30/30] or 80% RH [30/80]), duration (4, 8, 12 h), and feather cover (105 well-feathered [WF], 105 poorly-feathered [PF]). A total of 210 hens/replicate/farm (farm=block; 3 total) were tested during the simulated transport. Crates (one/duration/replicate), divided in half for each feather cover (seven hens/side), were placed in a climate-controlled chamber. Prior to exposure, hens were fasted (6 h). BW was taken pre- and post-exposure, and the difference was calculated as live shrink. Post-exposure to the test conditions, birds were slaughtered and carcasses were analyzed for muscle characteristics. Data were analyzed as a randomized complete block design (farm of origin as block) with ANOVA (Proc Mixed, SAS 9.4; significance declared at P ≤ 0.05). Duration resulted in more weight loss for the birds (P < 0.01). Final pH measures (30 h post-mortem) were higher in hens exposed to -10 than 21/80, 30/30, and 30/80 and this difference was exacerbated with time (breast P < 0.01 and thigh P = 0.01). For muscle color, breast and thigh (both feather covers; P = 0.01) were darker in the -10 treatment while redness values were higher in EOCH exposed to this treatment (breast and thigh P < 0.01). Additionally, thigh muscle redness was higher in PF hens (P < 0.01). Thaw and cooking losses were impacted by T/RH and duration (thaw loss P = 0.03 and cook loss P = 0.04). Cook loss was also influenced by T/RH and feather cover with PF hen muscles losing less water during cooking in the -10 treatment (P = 0.01). Overall, the largest impact from transport was found in hens exposed for a longer duration to -10 antemortem compared to other treatments, demonstrating a significant impact on muscle characteristics from ante-mortem stress.

The proposed international standard for infectious bronchitis vaccine (live).

An H-120 strain of infectious bronchitis virus was prepared in a form suitable to serve as an international standard for Infectious Bronchitis Vaccine (live). Accelerated degradation studies carried out on this vaccine showed it to be of suitable stability to serve as a standard. Results from a collaborative assay involving ten laboratories demonstrated that the proposed standard reduced the variation in virus titrations both within and between laboratories. This assay also showed that firstly the proposed standard could be used as a reference vaccine for the different safety tests described by the collaborating laboratories, and secondly as a reference vaccine in the different potency tests.

Comments on frontonasal dysplasia, ocular hypertelorism and dystopia canthorum.

The mean canthal index values of patients with frontonasal dysplasia are tested for differences in facies A, B, C, and D. A general discussion of ocular hypertelorism and dystopia canthorum is presented. Ocular hypertelorism is considered a sign which may occur in a variety of disorders. Quantitative methods for determining ocular hypertelorism are critically reviewed.

International reference preparation of anti-Mycoplasma gallisepticum serum.

The WHO Expert Committee on Biological Standardization requested the Central Veterinary Laboratory, Weybridge, England, to arrange a collaborative assay of material they had prepared to determine its suitability to serve as an international reference preparation of anti-Mycoplasma gallisepticum serum. Fourteen laboratories in eight countries performed comparative assays of the proposed reference preparation together with 3 immune sera from fowls. On the basis of the results obtained, the Committee established the preparation as the International Reference Preparation of Anti-Mycoplasma gallisepticum Serum and defined the International Unit as the activity contained in 0.0556 mg of the International Reference Preparation.