RESUMO
An H-120 strain of infectious bronchitis virus was prepared in a form suitable to serve as an international standard for Infectious Bronchitis Vaccine (live). Accelerated degradation studies carried out on this vaccine showed it to be of suitable stability to serve as a standard. Results from a collaborative assay involving ten laboratories demonstrated that the proposed standard reduced the variation in virus titrations both within and between laboratories. This assay also showed that firstly the proposed standard could be used as a reference vaccine for the different safety tests described by the collaborating laboratories, and secondly as a reference vaccine in the different potency tests.
Assuntos
Bronquite/veterinária , Galinhas , Coronaviridae/imunologia , Vírus da Bronquite Infecciosa/imunologia , Doenças das Aves Domésticas/prevenção & controle , Vacinas Virais/normas , Animais , Bronquite/prevenção & controle , Cooperação InternacionalAssuntos
Formação de Anticorpos , Galinhas/imunologia , Cobaias/imunologia , Orthomyxoviridae/imunologia , Coelhos/imunologia , Vacinas Virais , Adjuvantes Imunológicos/administração & dosagem , Animais , Antígenos Virais/administração & dosagem , Bronquiolite Viral/veterinária , Feminino , Testes de Inibição da Hemaglutinação , Doenças dos Cavalos , Cavalos , Injeções Intramusculares , Masculino , Infecções por Orthomyxoviridae/veterinária , Fatores de Tempo , Vacinas Virais/administração & dosagemRESUMO
The WHO Expert Committee on Biological Standardization requested the Central Veterinary Laboratory, Weybridge, England, to arrange a collaborative assay of material they had prepared to determine its suitability to serve as an international reference preparation of anti-Mycoplasma gallisepticum serum. Fourteen laboratories in eight countries performed comparative assays of the proposed reference preparation together with 3 immune sera from fowls. On the basis of the results obtained, the Committee established the preparation as the International Reference Preparation of Anti-Mycoplasma gallisepticum Serum and defined the International Unit as the activity contained in 0.0556 mg of the International Reference Preparation.