RESUMO
Hepatitis A virus (HAV) was isolated directly from human faeces in PLC/PRF/5 cells. In the first passage cell-bound and supernatant viruses were found by immune electron microscopy and by enzymeimmunoassay. Serial passaging of HAV in PLC/PRF/5 cells resulted in its adaptation to the cell line and in reduction of the incubation time. HAV was still detectable after 10 cell passages. Cell-bound as well as supernatant HAV were employed as antigens in anti HAV IgM-enzymeimmunoassay.
Assuntos
Hepatovirus/crescimento & desenvolvimento , Adulto , Antígenos Virais/análise , Antígenos Virais/imunologia , Carcinoma Hepatocelular , Linhagem Celular , Fezes/microbiologia , Feminino , Hepatite A/microbiologia , Anticorpos Anti-Hepatite A , Antígenos da Hepatite A , Anticorpos Anti-Hepatite/análise , Hepatovirus/imunologia , Hepatovirus/isolamento & purificação , Humanos , Técnicas Imunoenzimáticas , Imunoglobulina M/análise , Neoplasias Hepáticas , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Cultura de VírusRESUMO
The authors analyse the behaviour of the circulating immune complexes in acute virus hepatitides with and without HBs antigenaemia in correlation with the bilirubin level and the transaminases. No essential differences were found depending on the severity of the lesion of the liver parenchyma and on the proof of the HBs-antigen. Constantly increased immune complexes were found during the whole period of observation. It seems that 4 weeks after the beginning of the disease a normalisation of the immunogenic reactivity and an end of the elimination of the causative organism did not yet appear.