RESUMO
Objetivo: o presente trabalho objetivou identificar o conhecimento dos professores da rede municipal de educação sobre os riscos ocupacionais do seu ambiente de trabalho e as causas dos seus adoecimentos. Método: estudo transversal, quantitativo, realizado com 33 professores da rede municipal de ensino que foram afastados por doença relacionada ao trabalho em 2013. O estudo seguiu os preceitos ético-legais e recebeu sua aprovação por meio do protocolo CAAE: 30585114.5.0000.5083. Resultados: entre os entrevistados 90,9% são do sexo feminino, com média de 17,13± 4,99 anos de profissão, média de idade de 45,79±9,82, média de afastamento de 28,45 ± 38,05 dias/ano. A variável frequência do adoecimento mostrou significância quando comparada ao desenvolvimento de prevenção de doenças no trabalho (p=0,016); 66,7% reconhecem os riscos ocupacionais no ambiente laboral, ocorrendo adoecimentos por riscos ergonômicos e psicossociais (33,3%). Todas as categorias cruzadas entre atestados e relatos dos trabalhadores mostraram bom entendimento para as causas de afastamentos, sendo respiratório (p=0,02), psicossocial (p=0,001), osteomuscular (p=0,001), ginecológico (p=0,001). Conclusão: professores reconhecem a existência dos riscos ocupacionais no ambiente de trabalho com ênfase no risco ergonômico e
Objective: this study aimed to identify the knowledge of teachers of municipal education on occupational hazards of their work environment and the causes of their illnesses. Method: cross-sectional, quantitative study conducted with 33 teachers of public schools that have been removed by occupational disease in 2013. The study followed the ethical and legal precepts and received approval by the CAAE protocol: 30585114.5.0000.5083 Results: of respondents, 90.9% are female, with a mean of 17.13 ± 4.99 years profession, a mean age of 45.79 ± 9.82, departure from average 28.45 ± 38.05 days /year. The variable frequency of illness showed significance when compared to the development of disease prevention at work (p = 0.016); 66.7% recognize the occupational hazards in the work environment, occurring illnesses for ergonomic and psychosocial risks (33.3%). All categories cross between certificates and workers' reports showed good understanding of the causes of sick leave, and respiratory (p = 0.02), psychosocial (p = 0.001), musculoskeletal (p = 0.001), gynecological (p = 0.001). Conclusion: teachers recognize the existence of occupational hazards in the workplace with an emphasis on ergonomic and psychosocial risk conduct contingency measures and do not wish to change their area of operation.
Objetivo: este estudio pretende identificar los conocimientos de los profesores de la educación municipal en la occupational hazards de su ambiente de trabajo y las causas de su adoecimentos. Método: estudio transversal, cuantitativo, realizado con 33 profesores de la enseñanza municipal que estaban destrozadas por enfermedades relacionadas con el trabajo en 2013. El estudio siguió los preceptos ético-legal y recibió la aprobación a través del Protocolo de CAAE: 30585114.5.0000.5083. Resultados: de los encuestados, 90.9% son mujeres, con promedio de 17.13 ± 4,99 años de profesión, edad media de 45.79 ± 9.82, eliminación de 28.45 promedio ± 38,05 días al año. La variable de la frecuencia de la enfermedad mostró significación en comparación con el desarrollo de la prevención de enfermedades en el trabajo (p = 0,016); 66.7% reconoce los peligros ocupacionales en el ambiente de trabajo, que se producen adoecimentos los riesgos ergonómicos y psicosociales (33.3%). Todas cruzadas de categorías entre certificados y cuentas de los trabajadores demostraron la comprensión de las causas de las salidas y vías respiratorias (p = 0,02), psicosocial (p = 0,001), musculoesqueléticos (p = 0,001), ginecológicas (p = 0,001). Conclusión: profesores reconocen la existencia de los riesgos profesionales en el ambiente de trabajo con énfasis en lo psicosocial y ergonómico riesgo medidas de prevención y no desea cambiar su área de especialización.
Assuntos
Riscos Ocupacionais , Doença , DocentesRESUMO
Epigenetic silencing of transgene expression represents a major obstacle for the efficient genetic modification of multipotent and pluripotent stem cells. We and others have demonstrated that a 1.5 kb methylation-free CpG island from the human HNRPA2B1-CBX3 housekeeping genes (A2UCOE) effectively prevents transgene silencing and variegation in cell lines, multipotent and pluripotent stem cells, and their differentiated progeny. However, the bidirectional promoter activity of this element may disturb expression of neighboring genes. Furthermore, the epigenetic basis underlying the anti-silencing effect of the UCOE on juxtaposed promoters has been only partially explored. In this study we removed the HNRPA2B1 moiety from the A2UCOE and demonstrate efficient anti-silencing properties also for a minimal 0.7 kb element containing merely the CBX3 promoter. This DNA element largely prevents silencing of viral and tissue-specific promoters in multipotent and pluripotent stem cells. The protective activity of CBX3 was associated with reduced promoter CpG-methylation, decreased levels of repressive and increased levels of active histone marks. Moreover, the anti-silencing effect of CBX3 was locally restricted and when linked to tissue-specific promoters did not activate transcription in off target cells. Thus, CBX3 is a highly attractive element for sustained, tissue-specific and copy-number dependent transgene expression in vitro and in vivo.
