Assuntos
Fagocitose , Placenta/imunologia , Feminino , Ouro , Humanos , Técnicas In Vitro , Perfusão , Placenta/citologia , Gravidez , Trofoblastos/imunologiaRESUMO
The binding and internalization of colloidal gold-labelled insulin complexes into cultivated human arterial smooth muscle cells was studied. We could demonstrate that the colloidal gold-labelled insulin complex was bound to specific receptors on the cell surface of smooth muscle cells and internalized after 30 min at 20 degrees C. The gold-labelled insulin complexes entered the cell via vesicles and escaped from them in the cytoplasm. The biological activity of the complexes was determined by measuring their effect on DNA synthesis in smooth muscle cells and incorporation of (14C)-glucose into isolated fat cells. The binding characteristics of the complexes were examined by competitive inhibition of 125I-insulin binding to isolated fat cells in the presence of increasing concentrations of gold-labelled insulin complexes or unlabelled insulin. The gold-labelled insulin complex showed a growth promoting and metabolic effect and was able to compete with 125I-insulin for its binding sites.
Assuntos
Insulina/metabolismo , Músculo Liso Vascular/metabolismo , Tecido Adiposo/metabolismo , Animais , Células Cultivadas , Ouro Coloide Radioativo/metabolismo , Humanos , Masculino , Microscopia Eletrônica , Ratos , Ratos EndogâmicosRESUMO
The optic gland of the octupus responds to a variety of foreign proteins--1 to 2 days after the injection--by the mass production and release of a particulated material. In the electron microscope the antigen-induced substance shows as a non-membrane bound particle of size about 30 nm. When released into the blood it does not disintegrate instantly. The chemistry of this material, which is probably identical with the optic gland hormone, is not known. However, non-vesicular storage and release as well as the abundance of tubular mitochondria suggest that the hormone is a steroid. The optic gland hormone, which is known to control sexual maturation, feeding and death in Octopus, appears to be involved in a defence mechanism against non-octopus proteins.
Assuntos
Antígenos , Proteínas Sanguíneas/farmacologia , Glândulas Endócrinas/metabolismo , Olho/metabolismo , Ferritinas/farmacologia , Albumina Sérica/farmacologia , Animais , Glândulas Endócrinas/efeitos dos fármacos , Glândulas Endócrinas/ultraestrutura , Olho/efeitos dos fármacos , Feminino , Histocitoquímica , Humanos , Imunodifusão , Masculino , OctopodiformesRESUMO
Horse spleen ferritin, injected into the blood of the octopus, leaves the capillaries via pericyte junctions and windows. One hour after the administration, ferritin has entered the optic gland main cells; three hours later, it is accumulated in dense-bodies. The evidence for resorption supports earlier papers reporting that the ultrastructure of the organ is unusual for an endocrine gland.
Assuntos
Glândulas Endócrinas/ultraestrutura , Octopodiformes/ultraestrutura , Animais , Transporte Biológico , Glândulas Endócrinas/metabolismo , Feminino , Ferritinas/metabolismo , Gonadotropinas Hipofisárias , MasculinoRESUMO
The subpeduncualte lobe of the octopod brain produces a hormone that is released into the orbit. The hormone was extracted from Eledone cirrosa and assayed on the isolated perfused heart of the same species. It has a remarkable effect on amplitude, beat frequency and blood pressure; compared to equivalent extracts of posterior salivary gland it is about 70 times more effective. The substance is not a biogenic amine.
