Gonadotropin priming before OPU: What are the benefits in cows and calves?

Oocyte pick up (OPU) coupled with IVP produce over 1 million cattle embryos per year and has been most successful in Bos indicus derived breeds that contain large numbers of antral follicles on their ovaries. More recently, this technology has been applied on a large scale to Bos taurus cattle, where hormone manipulation is generally employed to improve the developmental competence of the COCs. Hormone manipulation, and specifically the use of FSH priming before OPU, has been strategically used in the intensively managed dairy cow, where genomic evaluation and juvenile IVP can produce additional significant genetic gains.

Targeting epigenetic nuclear reprogramming in aggregated cloned equine embryos.

Epigenetic perturbations during the reprogramming process have been described as the primary cause of the low efficiency of somatic cell nuclear transfer (SCNT). In this study, we tested three strategies targeting nuclear reprogramming to investigate effects on equine SCNT. First, we evaluated the effect of treating somatic cells with chetomin, a fungal secondary metabolite reported to inhibit the trimethylation on histone 3 lysine 9 (H3K9 me3). Second, caffeine was added to the culture medium during the enucleation of oocytes and before activation of reconstructed embryos as a protein phosphatase inhibitor to improve nuclear reprogramming. Third, we tested the effects of the histone deacetylase inhibitor trichostatin A (TSA) added during both activation and early embryo culture. Although none of these treatments significantly improved the developmental rates of the invitro aggregated cloned equine embryos, the first equine cloned foal born in Australia was produced with somatic cells treated with chetomin. The present study describes the use of chetomin, caffeine and TSA for the first time in horses, serving as a starting point for the establishment of future protocols to target epigenetic reprogramming for improving the efficiency of equine cloning. Cloning is an expensive and inefficient process, but has gained particular interest in the equine industry. In this study we explored different strategies to improve cloning efficiency and produced the first cloned foal born in Australia. Our data serve as a starting point for the establishment of future protocols for improving equine cloning efficiency.