1.
Lab Chip
; 12(6): 1151-6, 2012 Mar 21.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22318451
RESUMO
The copper-catalyzed alkyne-azide cycloaddition (CuAAC) reaction was applied as the novel method of DNA immobilization on a modified solid support. The CuAAC click reaction enables the covalent binding of DNA modified with pentynyl groups at its 5'-end to azide-loaded slides. Click microarrays were produced using this approach and successfully employed in biological/model experiments.