RESUMO
Background: Since 1964, there has been a scarcity of reported cases of primary ameloblastoma (AM) or ameloblastic carcinoma (AMCa) of the skull. The clinical presentation and distinctive features of this uncommon condition at specific anatomical sites remain unclear. We report a case of malignant transformation of a primary AM of the skull situated in the frontal-temporal-parietal region and highlight its similarities to other cases reported in the literature. Clinical presentation: A 53-year-old female patient presented with a 20-day history of headaches and bilateral lower limb weakness for 10 days. Physical examination revealed slow and unsteady gait. An occupying lesion was observed in the right frontal-temporal-parietal region of the skull on the Cranial imaging. A right cranial bone tumor margin expansion resection was performed. The patient's motor functions recovered normally after surgery. Postoperative imaging examinations showed10 tumor resection. Follow-up imaging examinations showed tumor recurrence. The patient underwent resection of the recurrent tumor. Postoperative pathological analysis revealed malignant transformation of the AM.Follow-up imaging examinations showed tumor recurrence again. The patient was admitted for stereotactic radiotherapy. Follow-up imaging examinations demonstrated no evidence of tumor recurrence and subsequent chest CT revealed no signs of metastasis. Conclusion: Primary AM or AMCa of the skull is increasingly being described in the literature, but detailed reports on the malignant transformation of primary AM of the skull are lacking. The pathogenesis of this condition remains unclear. Aggressive treatment and close follow-up may be crucial for preventing disease recurrence and malignant transformation.
RESUMO
A dual-wavelength microarray scanner based on laser confocal principle is constructed to acquire the laser-induced two-color fluorescence from dyes Cy3 and Cy5. Unlike most commercially available micoarray scanners whose lasers are continuous wave, filters are bandpass and the channels are multiple photomultiplier tubes (PMTs), the system mainly consists of two diode lasers, four volume holographic band-stop filters, and one PMT. The central wavelengths of two kinds of volume holographic band-stop filters are equal to those of the two laser, respectively. The fluorescence beams can pass through the filters, while laser beams are blocked by them because the filters are cut off. The two fluorescences can pass through a common optical path. Thus, only one pinhole and one PMT are required. The two fluorescences are acquired by the PMT with time sharing. The two respective fluorescences can be gathered when two diode lasers work asynchronously. If lasers work synchronously, the two wavelength fluorescences could be acquired simultaneously by the same PMT. A synthesis of a two-color fluorescence image can be realized straightforwardly in this manner. An experiment is conducted and Cy3 and Cy5 fluorescences are captured. Gridding of the fluorescent images based on basic morphological erosion appropriately locates the boundary between adjacent spots.
