Manure derived hydrochar reduced phosphorus loss risk via an alteration of phosphorus fractions and diversified microbial community in rice paddy soil.

Phosphorus (P) loss caused by the irrational use of manure organic fertilizer has become a worldwide environmental problem, which has caused a potential threat to water safety and intensified agricultural non-point source pollution. Hydrothermal carbonization is method with a low-energy consumption and high efficiency to deal with environmental problems. Application of pig manure-derived hydrochar (PMH) to soil exhibited potential of sustainable development compared with the pristine pig manure (PM). However, the effects of PMH on the distribution of P among the fractions/forms and the interaction between microorganisms and P forms and its relevance to the potential loss of P in paddy fields has not been clarified. Therefore, in this study, a soil column experiment was conducted using the untreated soil (control), and the PM, PMH1 (PMH derived at 180 °C), and PMH2 (PMH derived at 260 °C) treated soils (at the dose of 0.05 %) and rice was cultivated to investigate the effects of PM and PMH on the P fractions, mobilization, ad potential loss via the induced changes on soil microbial community after a complete growing season of rice. The trend of P utilization was evaluated by P speciation via continuous extraction and 31P NMR. The addition of PMH reduced the proportion of residual P in soil by 23.8-26.3 %, and increased the proportion of HCl-P and orthophosphate by 116.2-158.6 % and 6.1-6.8 % compared to PM. The abundance of gcd gene developed after the application of PMH2, which enhanced the mobile forms of soil P utilization via secreting gluconic acid. The network diagram analysis concluded that the changes in various P forms were mainly related to Proteobacteria, Bacteroides, Firmicutes and Acidobacteria. The results illustrated that PMH mitigate the potential risk of P loss more than PM by altering P fractions and affecting soil microbial community.

Manure-derived hydrochar superior to manure: Reducing non-point pollution risk by altering nitrogen and phosphorus fugacity in the soil-water system.

Hydrothermal carbonization (HTC) technology is an emerging technology for the disposal of manure-based wet wastes. However, the effects of manure-derived hydrochar inputs to agricultural soils on nitrogen (N) and phosphorus (P) morphology and conversion in soil-water systems remain largely unexplored. In this study, pig and cattle manure (PM and CM), and their derived hydrochar (PCs and CCs) were applied to agricultural soils, with changes in nutrient morphology and enzyme activities related to N and P transformation in the soil-water systems observed through flooded incubation experiments. The results showed that floodwater ammonia N concentrations were reduced by 12.9-29.6% for PCs relative to PM, and 21.6-36.9% for CCs relative to CM, respectively. Moreover, floodwater total P concentrations of PCs and CCs were reduced by 11.7-20.7% relative to PM and CM. Soil enzyme activities closely related to N and P transformations in the soil-water system responded differently to manure and manure-derived hydrochar application. Compared to manure, the application of manure-derived hydrochar inhibited soil urease and acid phosphatase activity by up to 59.4% and 20.3%, respectively, whereas it had significant promotion effects on soil nitrate reductase (∼69.7%) and soil nitrite reductase (∼64.0%). The products of manure after HTC treatments have the characteristics of organic fertilizers, and the fertilization effects of PCs are more prominent than CCs, which are subject to further verification in field trials. Our findings improve the current understanding of manure-derived organic matter affecting N and P conversions in soil-water systems and the risk for non-point source pollution.

Corrigendum: Coexpression of HHLA2 and PD-L1 on tumor cells independently predicts the survival of spinal chordoma patients.

[This corrects the article DOI: 10.3389/fimmu.2021.797407.].

Effect of sulfamethazine on anaerobic digestion of manure mediated by biochar.

Antibiotic contamination from animal production and wastewater treatment process will release antibiotic resistant genes to the environment and potentially threaten human health. Meanwhile, the residual antibiotic in manure could have inactive impacts on anaerobic digestion (AD). This study explores the effect of sulfamethazine on manure AD mediated by biochar. The results show that biochar weakens the adverse effects of sulfamethazine on AD by adsorption sulfamethazine during the initial stage (0-3 days) of AD and promoting the growth of hydrolytic bacteria (especially Firmicutes and Bacteroidetes) and methanogens (especially Methanothrix and Methanosarcina). Besides, the presence of biochar improves the biogas production capacity of AD and promotes microbial diversity and community richness. Thus, the addition of biochar greatly reduces sulfamethazine and is testified to be a desirable strategy to mitigate the inhibition of sulfamethazine on AD.

Effect of the near-infrared activated photocatalyst Cu2(OH)PO4 nanoparticles on the growth of harmful algal blooms causing Microcystis aeruginosa.

The wide range existence of M. aeruginosa FACHB 905 strains in the aquatic environment becomes a great threat for the health of humans and animals; it also poses a great obstacle in the ecological ecosystem. Therefore, an effective, efficient, and environmentally friendly method of treatment is needed. In this work Cu2(OH)PO4 nanoparticles were successively synthesized from a mixture of Cu (NO3)2 and Na2HPO4 according to the results from Fourier-transform infrared (FT-IR), X-ray diffraction (XRD), ultraviolet/visible/near-infrared in diffuse reflectance spectroscopy (UV/Vis/NIR DRS), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS) tests. Furthermore, Cu2(OH)PO4 was used to mitigate the growth of M. aeruginosa FACHB 905 strains on a lab-scale, and the investigation on the growth of the harmful algal bloom (HAB) causing M. aeruginosa FACHB 905 strains was worked on. The Cu2(OH)PO4 is effective in inhibiting the growth of the strain by more than 97% at a concentration of 0.032 mg mL-1. Furthermore, analysis of the chlorophyll a content and polysaccharide asserted that a remarkable decrease from 9.40 mg L-1 and 37.66 mg L-1 for the control to 0.07 mg L-1 and 10.21 mg L-1 for the treatment media with 0.032 mg mL-1 Cu2(OH)PO4 has been achieved. The results affirm the effectiveness of the Cu2(OH)PO4 as suitable candidates for preventing HABs caused by the M. aeruginosa FACHB 905 cyanobacterium and other similar strains.

Anaerobic fermentation treatment improved Cd2+ adsorption of different feedstocks based hydrochars.

Hydrothermal carbonization technology has attracted wide attention in recent years owing to its advantages, e.g., high yield and clean production, compared with traditional pyrolysis. Anaerobic fermentation (AF) is a new method to modify carbon materials, which may improve the surface properties of hydrochar (HC). To explore whether AF has effects on different feedstocks based HCs, two kinds of HCs derived from wheat straw and poplar sawdust were treated with AF for different time in this study. By comparing the changes in physicochemical properties of anaerobic fermentative hydrochars (AFHCs), adsorption behaviors of Cadmium (Cd2+) on AFHCs were evaluated. The results showed that the surface electrical characteristics, specific surface area, and oxygen-containing functional groups of HCs improved significantly after AF treatment, which confirmed our hypothesis that AF is suitable for improving the adsorption of different feedstocks based HCs. The adsorption capacity of Cd2+ on AFHCs was significantly enhanced by a 3.1-3.4 times increase after AF treatment. The effect of AF treatment on wheat straw hydrochar (WHC) was more evident than poplar sawdust hydrochar (SHC). WHCs treated with AF own higher adsorption capacity of Cd2+, which was attributed to the higher negative charge, more exchangeable cations, and more oxygen-containing functional groups. The adsorption process was found to be a spontaneous endothermic reaction dominated by chemisorption and controlled by electrostatic attraction, ion exchange, functional groups complexation, and π-bonding coordination. These results were contributed to understanding the modification of HC by AF and its application in heavy metal pollution remediation.

Coexpression of HHLA2 and PD-L1 on Tumor Cells Independently Predicts the Survival of Spinal Chordoma Patients.

Background: Immunotherapy only achieves efficacy in some cancer patients, and less is known about other immune checkpoint molecules in chordoma. Here, we aimed to determine the expression of PD-L1, HHLA2, B7H3, IDO-1 and Galectin-9 in spinal chordoma and evaluated their association with tumor infiltrating lymphocytes (TILs), clinicopathological characteristics and survival of patients. Methods: Using multiplexed quantitative immunofluorescence (QIF), we simultaneously measured the levels of five different immune checkpoint molecules and major TIL subsets in 92 human spinal chordoma samples. Results: Tumor HHLA2 and PD-L1 were positive in 80.0% and 86.0% of cases, respectively. However, B7H3, IDO-1 and Galectin-9 positivity on tumor cells were only seen in 21.0% of cases, despite all showing predominantly stromal expression. Coexpression of these QIF markers in the tumor compartment was scarcely detected except for PD-L1 and HHLA2, which was observed in 69.6% of cases. While tumoral HHLA2 and stromal B7H3 expressions were associated with an aggressive tumor phenotype, suppressive immune response (specifically including elevated PD-1+ TILs level and decreased CD8+ TIL density) and poor prognosis, stromal levels of PD-L1 and Galectin-9 predicted the opposite outcomes. Importantly, HHLA2 and PD-L1 coexpression on tumor cells independently predicted both worse local recurrence-free survival and overall survival. Conclusion: These data provide a better understanding of the immunosuppressive mechanism in chordoma and may be useful for the development of combination or novel immunotherapy approaches aiming to improve therapeutic efficacy and survival.

Physicochemical properties of aged hydrochar in a rice-wheat rotation system: A 16-month observation.

The importance of hydrochar properties for soil application is well known, but the effects of natural aging on hydrochar properties remain ambiguous. The present study aimed to determine the shift patterns in the physicochemical properties of hydrochar through a 16-month soil column aging experiment conducted in a rice-wheat rotation system with hydrochars derived from a wheat straw at 220 °C and 260 °C. Obvious decreasing hydrophilic/polarity indices and increasing porosity, ash content, and stability occurred in aged hyrdrochar, which were due to the dissolved organic matter (DOM) leaching and the interaction with mineral content and fertilizer during the 16-month aging process. Besides, fewer C-OH, slightly more CO, and higher aromaticity (C-C/CC) in aged hydrochar were observed. Meanwhile, the relative abundance of the compounds containing only C, H, and O atoms in water extract of aged hydrochar decreased, while that of the compounds containing C, H, O, and N atoms increased during aging; these findings were attributed to the less labile DOM and microbial degradation and the retention of some plant-derived dissolved organic carbon, respectively. This study provided 16-month aging characterization data regarding alteration in hydrochar physicochemical properties, which was conducive to make a better understanding of the use of hydrochars as sustainable soil amendments from agroecosystems and environmental perspective.

Maternal and neonatal outcomes after exposure to ADHD medication during pregnancy: A systematic review and meta-analysis.

PURPOSE: Attention-deficit/hyperactivity disorder (ADHD) medications are used by increasing numbers of reproductive-age women. The safety of these medications during pregnancy has not been well described. METHODS: A systematic review and meta-analysis was performed to evaluate the adverse maternal and neonatal outcomes associated with exposure to ADHD medication during pregnancy. The PubMed and Embase databases were searched to identify potential studies for inclusion. RESULTS: Eight cohort studies that estimated adverse maternal or neonatal outcomes associated with exposure to ADHD medication during pregnancy were included. Exposure to ADHD medication was associated with an increased risk of neonatal intensive care unit (NICU) admission compared with no exposure at any time (risk ratio (RR) 1.88; 95% confidence interval (CI), 1.7-2.08) and compared with women with exposure either before or after pregnancy (RR 1.38; 95% CI, 1.23-1.54; P < 0.001). Exposure to methylphenidate (MPH) was marginally associated with an increased risk for cardiac malformation (RR 1.27; 95% CI, 0.99-1.63; P = 0.065) compared with no exposure. However, exposure to ADHD medication was not associated with an increased risk for other adverse maternal or neonatal outcomes. This analysis was limited by the small number of studies included and the limited adjustments for the possible confounders in the studies. CONCLUSIONS: Exposure to ADHD medication during pregnancy does not appear to be associated with adverse maternal or neonatal outcomes. Given the few studies included, further larger, prospective studies that control for important confounders are needed to verify our findings.

Effects of different concentrations of isoflurane pretreatment on respiratory mechanics, oxygenation and hemodynamics in LPS-induced acute respiratory distress syndrome model of juvenile piglets.

PURPOSE: This study was prospectively designed to investigate the effects of different concentrations of isoflurane (ISO) pretreatment on respiratory mechanics, oxygenation, and hemodynamics in lipopolysaccharide (LPS)-induced acute respiratory distress syndrome (ARDS) model of juvenile piglets. METHODS: Twenty-four piglets (9-14 kg, 5-6 weeks old) were randomly assigned to four groups (n = 6): LPS group, which was injected with LPS (20 µg/kg) to induce ARDS; 0.5 ISO-LPS, 1.0 ISO-LPS, and 1.3 ISO-LPS groups, which were pretreated with 0.5, 1.0, and 1.3 minimum alveolar concentrations (MAC) ISO for 30 min before immediate LPS infusion, respectively. After establishment of ARDS, respiratory mechanism, oxygenation and hemodynamics parameters were measured at baseline, and 0, 1, 2, 3, and 4 hours after induction of ARDS. RESULTS: After induction of ARDS, there were increases in alveolar-arterial oxygen difference (A-aDO2), oxygenation index (OI), mean airway pressure (MAP), dead space-to-tidal volume ratio, heart rate (HR), dP/dtmax, extravascular lung water index, pulmonary vascular permeability index, and PaCO2, and decreases in PaO2/FIO2, respiratory rate (RR), dynamic lung compliance (Cdyn), mean arterial blood pressure (MABP) and systemic vascular resistance (SVR) compared with baseline (P(time) < 0.05). Pretreatment with 1.0 and 1.3 MAC ISO alleviated changes in dP/dtmax and PaCO2 at ARDS 0-2 hours, SVR at 0-3 hours, PaO2/FIO2, RR, and MABP at 1-2 hours, HR at 2-3 hours, A-aDO2 at 3-4 hours, and OI at 4 hours (P(group) < 0.05). CONCLUSION: Pretreatment with 1.0 and 1.3 MAC ISO had protective effects on respiratory mechanics, oxygenation, and hemodynamics in piglets with LPS-induced ARDS.

Unanticipated ventilation obstruction due to a defective reinforced endotracheal tube.

Wire-reinforced endotracheal tubes are used to prevent obstruction. Risk factors related to reinforced endotracheal tube obstruction were believed to be repeatedly used tube and in presence of N2O. In our case, even in free of these risk factors, a delayed tube obstruction occurred with the progress of surgical duration. This delay suggests that the obstruction was caused by diffusion of warm air/oxygen into an initially small defect, especially as the duration of surgery progresses.

Analysis of expressed sequence tags (ESTs) from a normalized cDNA library and isolation of EST simple sequence repeats from the invasive cotton mealybug Phenacoccus solenopsis.

The cotton mealybug, Phenacoccus solenopsis Tinsley, is a serious and invasive pest. At present, genetic resources for studying P. solenopsis are limited, and this negatively affects genetic research on the organism and, consequently, translational work to improve management of this pest. In the present study, expressed sequence tags (ESTs) were analyzed from a normalized complementary DNA library of P. solenopsis. In addition, EST-derived microsatellite loci (also known as simple sequence repeats or SSRs) were isolated and characterized. A total of 1107 high-quality ESTs were acquired from the library. Clustering and assembly analysis resulted in 785 unigenes, which were classified functionally into 23 categories according to the Gene Ontology database. Seven EST-based SSR markers were developed in this study and are expected to be useful in characterizing how this invasive species was introduced, as well as providing insights into its genetic microevolution.

Rapid spread of a recently introduced virus (tomato yellow leaf curl virus) and its vector Bemisia tabaci (Hemiptera: Aleyrodidae) in Liaoning Province, China.

In Liaoning Province, China, tomato yellow leaf curl virus (TYLCV) was first detected in 2009 and in only four counties. To quantify the spread of TYLCV and to identify potential factors influencing its spread in Liaoning Province, we assayed for TYLCV within 1,055 whiteflies (Bemisia tabaci (Gennadius) complex) from 74 populations and 29 counties in 2011. The B. tabaci species of these individuals was determined based on molecular markers. TYLCV was found in 13 counties (Donggang, Liaoyang, Kazuo, Lingyuan, Heishan, Liaozhong, Kaiyuan, Taian, Dawa, Dashiqiao, Beizhen, Linghai, and Xingcheng) and was most frequently detected in the central plain. In addition, the percentage of whiteflies with TYLCV was significantly higher in B. tabaci Q than in B. tabaci B but was unrelated to the hosts (pepper, eggplant, tomato, cucumber, and kidney bean) on which the whiteflies had been collected. These results demonstrate that TYLCV has spread rapidly in Liaoning Province since its first detection and suggest that its spread is more closely associated with the introduction of B. tabaci Q than with the species of host plant. These findings also indicate that controls are now needed to reduce the further spread of TYLCV and that these controls should include the management of B. tabaci Q populations.

Airway management in pediatric patients undergoing suspension laryngoscopic surgery for severe laryngeal obstruction caused by papillomatosis.

OBJECTIVES: To review perioperative airway management and ventilation strategy during the surgical removal of papilloma under suspension laryngoscopy in pediatric patients with severe airway obstruction. METHODS: Seventy pediatric patients with degree III and IV laryngeal obstruction who underwent suspension laryngoscopy to remove laryngeal papillomatosis, between July 2005 and March 2009, were included in the study. All patients were intubated initially to secure the airway. Controlled ventilation through an endotracheal (ET) tube was used during the papilloma debulking near the glottis vera. Spontaneous ventilation or apneic technique was adopted based on the stage of the surgical procedure and the location of the remaining tumor. Hemodynamic parameters, pulse oxygen saturation (SpO(2)), and CO(2) were closely monitored, and adverse events were recorded. RESULTS: The duration of the surgical operation and the duration of the extubation period were 5-35 min and 5-20 min, respectively. Thirty cases with degree III and twenty cases with degree IV laryngeal obstruction received inhalation induction. Sixteen cases with degree III laryngeal obstruction were given an intravenous induction. Four patients admitted with a comatose status were emergently intubated without any anesthetics. The ET tube size was determined by assessing the opening through the tumor mass or glottic aperture under direct laryngoscopy. SpO(2) was maintained above 97% after the airway was secured and sufficient ventilation established. Controlled ventilation was used in all children during the bulk removal of tumor. Spontaneous respiration and apneic technique were adopted for the removal of the remaining tumor in the hypolarynx or trachea in 16 and 28 cases, respectively. Three patients had to be re-intubated postoperatively because of persistent desaturation or laryngospasm. CONCLUSION: Key points of perioperative airway management in pediatric patients with papillomatosis-induced severe laryngeal obstruction include careful preoperative airway evaluation; the proper choice of induction methods, and ET tube size; maintenance of an adequate depth of anesthesia; and flexible ventilation strategy, continuous and close monitoring during the extubation and postextubation period; and prompt management of adverse events.

[Wolbachia endosymbionts and their effects on the fitness of the arthropod hosts].

Wolbachia are common and maternally inherited bacteria found in reproductive tissue of a wide range of arthropod species. A tremendous amount of progress on their manipulating reproduction of their host has been made over the past 30 years. Recent surveys have found that they could effect the fitness of their hosts. The recent advances on Wolbachia distribution, locality and their effects on the fitness of hosts are reviewed, and the significance and potential implications of the fields are discussed.