RESUMO
OBJECTIVE: To analyze the results of unqualified blood screening among blood donors in Shaoxing region. METHODS: 257 145 blood samples from volunteer blood donors from 2017 to 2021 were tested for HBsAg, HCV, HIV, TP, ALT, HTLV, and NAT. All blood test results were analyzed retrospectively. RESULTS: Over the 5-year period, the average failure rate of the tests for volunteer blood donors was 1.22%. In the descending order from high to low, the positive rates of ALT, HBsAg, NAT, TP, HCV, HIV, HTLV were 0.32%, 0.25%, 0.20%, 0.19%, 0.17%, 0.07%, and 0.01% respecitively. Of these, HBsAg (χ2=65.23), ALT (χ2=47.32), and HCV (χ2=12.73) were significantly different between different years (P <0.05), but TP (χ2=4.19), HIV (χ2=7.58), NAT (χ2=7.62), and HTLV (χ2=6.75) were no significant differences (P >0.05). And the positivity rates of HBsAg and HCV was a trend of decreasing. By comparing nucleic acid and enzyme immunoassay positive tests, the two methods were found to complement each other. The age and gender distribution difference of the positive population could help to better ensure safe blood recruitment. CONCLUSION: It is necessary to strengthen blood testing efforts and continuously pay attention to the changing patterns of positive blood-borne diseases in order to improve blood quality and safety.
Assuntos
Infecções por HIV , Hepatite C , Humanos , Antígenos de Superfície da Hepatite B , Estudos Retrospectivos , Doadores de Sangue , Testes Hematológicos , Infecções por HIV/diagnóstico , Infecções por HIV/epidemiologia , Voluntários , Hepatite C/diagnósticoRESUMO
OBJECTIVE: To assess the molecular biological characteristics of occult hepatitis B virus (HBV) infected blood donors in Shaoxing. METHODS: 8692 blood donors were screened using ELISA. The occult HBV infection was determined by DNA analysis among the HBsAg negative subjects. DNA sequencing and mutational analysis were further performed in the HBV DNA positive samples. The overall situation of occult HBV infection was hereby evaluated and the possible underlying mechanisms discussed. RESULTS: Among the 8644 HBsAg negative subjects out of 8692 blood donors, 8 were HBV DNA positive. The occult HBV infection rate was 0.92 per thousand (8/8692). Among the 8 samples, 6 were genotype C (75%) and 2 genotype B (25%). In addition, a specific mutation in "a" epitope was observed in 7 OBI virus strains by amino acid analysis. CONCLUSION: There were occult HBV infected among blood donors in Shaoxing, which is probably associated with the gene mutation of HBV virus.
Assuntos
Doadores de Sangue , Vírus da Hepatite B/genética , Hepatite B/virologia , Adolescente , Adulto , Análise Mutacional de DNA , Feminino , Genótipo , Antígenos de Superfície da Hepatite B/sangue , Vírus da Hepatite B/classificação , Humanos , Masculino , Pessoa de Meia-Idade , FilogeniaRESUMO
OBJECTIVE: To investigate the incidence of HEV infection in population of non-remunerate blood donors in Shaoxing. METHODS: Blood specimens from 3701 non-remunerate blood donors were collected, ELISA were used to study anti-HEV IgG and IgM antibodies, RT-PCR were further used to study HEV RNA in samples from donors whose anti-HEV IgM was positive. RESULTS: Anti-HEV IgG positive rate was 29.19% (1107/3701), anti-HEV IgM positive rate was 1.35% (50/3701) among non-remunerate blood donors in Shaoxin. Six cases were positive for HEV RNA. The positive rate was 0.16% (6/3701), and all the 6 cases belonged to HEV genotype 1. Different seasons showed no interference on the positive rate of IgG and IgM. CONCLUSION: The detecting and studying of HEV infection among donors was important to ensure the safety of blood products and blood transfusion.
