Polymorphism of IFN-γ +874T/A associated with production of IFN-γ affects human papillomavirus susceptibility in rural women from Luohe, Henan, China.

PURPOSE: In this paper, the association between polymorphisms of IFN-γ +874T/A (rs2430561), IFN-γR1 -56 T/C (rs2234711), IFN-γR1 +95 C/T (rs7749390), and IFN-γR1 -611A/G (rs 1327474) and human papillomavirus (HPV) susceptibility was investigated in rural women from Luohe, Henan, China. PATIENTS AND METHODS: A total of 520 rural women were enrolled from Luohe, including 260 with HPV infection and mild dysplasia or less and 260 without HPV infection. Single-nucleotide polymorphisms (SNPs) of IFN-γ +874T/A, IFN-γR1 -56 T/C, IFN-γR1 +95 C/T and IFN-γR1 -611A/G were genotyped using TaqMan Pre-Designed SNP Genotyping Assays. Serum IFN-γ levels were measured using Human IFN-γ Quantikine ELISA Kit. Multivariate logistic regression analysis was performed to identify the SNPs associated with HPV susceptibility. Serum IFN-γ levels were compared between different genotypes. RESULTS: The polymorphism of IFN-γ +874T/A was associated with HPV susceptibility and +874A carriers had an increased risk. Moreover, the odds ratio was higher in +874 AA carriers than in +874 AT carriers (1.672 vs 2.874). Serum IFN-γ levels were highest in IFN-γ +874 TT carriers, intermediate in AT carriers, and lowest in AA carriers (2.86±1.14 vs 1.57±0.79 vs 0.41±0.22 pg/mL, all P<0.05). CONCLUSION: The polymorphism of IFN-γ +874T/A was associated with HPV susceptibility in rural women from Luohe, Henan, China, and +874A carriers had an increased risk. The possible mechanism was that +874A carriers had a low production of IFN-γ.

[Incidence and risk factors of stroke in patients with nonvalvular atrial fibrillation in Haikou].

OBJECTIVE: To investigate the incidence of ischemic stroke and associated risk factors in patients with nonvalvular atrial fibrillation (NVAF) in Haikou. METHODS: Area through follow-up approach and evaluate the effect of antithrombotic therapy as well as the current status of treatment in patients with NVAF. Clinical data of 329 NVAF patients admitted to Hainan hospital from January 1999 to December 2001 were collected. The patients were followed up to identify the incidence of ischemic stroke and associated risk factors. RESULTS: The main cause of NVAF was coronary heart diseases, which happened in 207 cases (62.9%). The logistic regression analysis revealed that age, type of atrial fibrillation, and hypertension were significantly associated with the occurrence of ischemic stroke in patients with NVAF (P=0.0001, 0.006, and 0.04 respectively). Antithrombotic treatments significantly decreased the incidence of stroke (P<0.05). But Warfarin was not applied consistently in Haikou. CONCLUSIONS: Age, type of atrial fibrillation and hypertension are independent risk factors for ischemic stroke in patients with NVAF. Uninterrupted use of aspirin can be beneficial to patients with NVAF.