Clinical research on minimally invasive rotary resection in the treatment of lactational breast abscess.

BACKGROUND: This study sought to compare the efficacy of minimally invasive rotatory resection plus catheter drainage and puncture abscess aspiration in the treatment of lactational breast abscess. METHODS: The data of 80 patients with lactational breast abscess at the Department of Mammary Gland, Tianjin Central Hospital of Gynecology Obstetrics, from January 2019 to January 2020 were retrospectively collected. The patients were divided into the minimally invasive group (n=30) and the abscess aspiration group (n=50). Clinical data, including age, parity, preoperative body temperature, postpartum time, time of abscess cavity generation, diameter and position of abscess cavity, intraoperative drainage, bleeding, recovery time, and satisfaction score, were collected and compared between the 2 groups of patients. RESULTS: The baseline data between the 2 groups were not significantly different. The minimally invasive group had a higher single drainage volume and single bleeding volume (P<0.05), and lower cumulative pain score (P<0.05); the cumulative bleeding volume did not differ significantly between the 2 groups (P>0.05). For the abscess cavities with a diameter ≤5 cm, the single drainage volume, single bleeding volume, and single pain score in the minimally invasive group were significantly higher than those in the abscess aspiration group (P<0.05); the total drainage volume was similar between the 2 groups (P>0.05). For the abscess cavities with a diameter >5 cm, the single drainage volume and single bleeding volume in the minimally invasive group were significantly higher than those in the abscess aspiration group (P<0.05), while the cumulative bleeding volume, single pain score, and cumulative pain score were lower in the minimally invasive group (P<0.05). There was no significant difference in total drainage between the 2 groups (P>0.05). CONCLUSIONS: When the abscess cavity is ≤5 cm in diameter and the abscess is superficial, puncture and abscess aspiration may be more advantageous than the minimally invasive rotatory resection plus catheter drainage. When the abscess cavity is >5 cm in diameter and there are multiple abscesses or deep large abscess, the efficacy of minimally invasive rotary resection plus catheter drainage may be better than the puncture and abscess aspiration.

Molecular Identification Based on Chloroplast Sequences and Anti-complementary Activity Comparison of Juniperus Samples from the Qinghai-Tibet Plateau.

Juniperus (Cupressaceae, Pinales) plants are widely distributed in the Qinghai-Tibet Plateau of China. The leaves and twigs of at least 8 Juniperus species (J. pingii, J. pingii var. wilsonii, J. squamata, J. recurva var. coxii, J. saltuaria, J. indica, J. tibetica and J. convallium var. microsperma) have been used as the Tibetan medicine Xuba. At present, it is difficult to distinguish among the original species of Xuba based only on their similar morphological characteristics. However, in our previous studies, 4 Xuba samples from different Juniperus species exhibited significant differences in both anticomplementary activity in vitro and anti-inflammatory effects on acute lung injury in vivo. To identify the effective original species of Xuba reliably, in this study, we developed a sequencing-based DNA molecular technology to distinguish 14 populations of 8 Juniperus species collected from Tibet region, using trnS-G, trnD - T, and petN-psbM genomic regions to build phylogenetic trees. In addition, their anticomplementary activities were evaluated. The results showed that combined sequence of these 3 genomic regions could identify 8 Juniperus species clearly and clustered individuals of one species but from different locations, whichever phylogenetic tree was constructed. Moreover, the anticomplementary activities of the 8 species were clustered into 2 groups. Among them, J. saltuaria and J. recurva var. coxii, which formed an independent branch apart from the other 6 species in phylogenetic trees, were the most potent (CH50: 0.029 - 0.032 mg/mL). Consequently, DNA identification of Juniperus using the combined sequence could provide beneficial guidance for further efficacy evaluation and quality control of Xuba.

Anti-complementary constituents of Anchusa italica.

Activity-guided fractionation for complement inhibitors led to the isolation of 24 known compounds from Anchusa italica. Chemical types include eight megastigmane compounds, five triterpenoid compounds, five lignan compounds, three flavonoid compounds, two alkaloid compounds and one phenthyl alcohol compound. Among which, a lignan (medioresinol), an alkaloid (5-hydroxypyrrolidin-2-one) and a flavonoid (5-hydroxyl-3', 4', 6, 7-tetramethoxy flavone) exhibited better anticomplementary effects with CH50 values ranging from 0.07 to 0.82 mM, which are plausible candidates for developing potent anticomplementary agents.

Anti-complementary constituents of Viola kunawarensis.

Activity-guided fractionation for complement inhibitors led to the isolation of 22 known compounds from Viola kunawarensis. Chemical types include six sterol compounds, three coumarin compounds, five megastigmane compounds, two triterpenoid compounds, two phenylpropanoid compounds, one chlorophyll, one amide, and two lipid compounds. Among which, two sterols (stigmasta-4-ene-3ß,6ß-diol and saringosterone), one amide (aurantiamide acetate) and a norsesquiterpenoid (solalyratin B) exhibited better anti-complementary effects with CH50 values ranging from 0.02 to 0.08 mM, which are plausible candidates for developing potent anti-complementary agents.