Satisfacción de usuarios de consultorios urbanos del Policlínico Norte, municipio Placetas, Villa Clara / User Satisfaction with Urban Family Medical Offices Belonging to the Norte Policlinic from Placetas Municipality, Villa Clara Province

Introducción: La satisfacción del usuario juega un rol protagónico, pues se convierte en el censor activo para evaluar la calidad del servicio. Objetivo: Determinar el grado de satisfacción de los usuarios de consultorios urbanos. Métodos: Se realizó un estudio descriptivo transversal que pertenece a las investigaciones en organización y eficiencia en los sistemas de salud. La población estuvo conformada por los 8371 pacientes mayores de 19 años del Policlínico Docente Norte del municipio Placetas en el año 2020. La muestra, seleccionada por muestreo estratificado aleatorio, quedó conformada por 124 sujetos previo consentimiento informado. Resultados: En la muestra predominó el grupo etario mayor de 60 años (42,7 por ciento); el sexo femenino (78,2 por ciento); el nivel escolar preuniversitario (52,4 por ciento); la presencia de enfermedad crónica (65,3 por ciento). El coeficiente de contingencia V de Cramer mostró que la satisfacción no guarda relación con la presencia de enfermedad crónica, que está parcialmente correlacionada con las condiciones estructurales y las informaciones para los pacientes. La correlación de Pearson mostró correlación parcial con los aspectos de la atención médica, excepto el tiempo de espera prolongado y el respeto a las creencias religiosas. Conclusiones: Los usuarios de consultorios urbanos de forma general se encuentran satisfechos, dependiendo dicha satisfacción de las condiciones estructurales, las informaciones adecuadas para los pacientes y los aspectos relacionados a la atención médica(AU)

Introduction: User satisfaction plays a leading role, since it becomes the active censor to evaluate the quality of service. Objective: To determine the degree of satisfaction of users with urban family medical offices. Methods: A cross-sectional and descriptive study was carried out as part of the research on organization and efficiency in health systems. The population consisted of the 8371 patients over 19 years of age from Norte Teaching Policlinic of Placetas Municipality in 2020. The sample, selected by stratified random sampling, consisted of 124 subjects with prior informed consent. Results: The sample was dominated by the age group older than sixty years (42.7 percent), the female sex (78.2 percent), the senior high school level (52.4 percent), as well as the presence of chronic disease (65.3 percent). The Cramer's V contingency coefficient showed that satisfaction is not related to the presence of any chronic disease, which is partially correlated with structural conditions and information for patients. Pearson's correlation showed partial correlation with aspects of medical care, except long waiting time and respect for religious beliefs. Conclusions: Users of urban family medical offices are generally satisfied, such satisfaction depending on structural conditions, adequate information for patients, and aspects related to medical care(AU)

Complete Genome Sequence of Mycobacteriophage Joy99.

Joy99 is a siphoviral mycobacteriophage with a 59,837-base pair double-stranded DNA genome and is predicted to contain 97 protein-coding genes and a single tRNA gene. Joy99 was isolated in Saint Louis, MO, and annotated by students at Bluff Dale High School in community engagement with Tarleton State University.

Satisfacción laboral en médicos de familia de un policlínico del municipio Placetas / Occupational Satisfaction in Family Doctors from a Polyclinic in Placetas Municipalities

Introducción: La satisfacción es mucho más que una aspiración humana, es un instrumento y una función para lograr la excelencia de la calidad en la atención en salud. Objetivo: Determinar el nivel de satisfacción laboral de médicos de la familia. Métodos: Se realizó un estudio descriptivo de corte transversal en el Policlínico Docente Norte de Placetas en el año 2019. El universo estuvo constituido por los 18 médicos de la familia del policlínico y con un muestreo no probabilístico, deliberado con carácter casuístico a partir de no haber contactado a algunos por difícil acceso quedó conformada la muestra por 15, teniendo como criterio de inclusión la voluntariedad de participar. Resultados: Predominaron como condiciones estructurales no adecuadas los servicios sanitarios (93,3 por ciento) y la privacidad de la consulta (73,3 por ciento); de las condiciones de trabajo propiamente dichas no adecuadas, los muebles de la consulta y sala de espera (86,6 por ciento); de los elementos relacionados con el diagnóstico y tratamiento, todos fueron no adecuados; de los factores subjetivos, el más referido fue el estrés (93,3 por ciento) y como grado de satisfacción, el poco satisfecho (80 por ciento). Conclusiones: Existe poca satisfacción laboral de los médicos del Policlínico Docente Norte de Placetas, determinado esto por un conjunto de factores interrelacionados entre sí(AU)

Introduction: Satisfaction is much more than a human aspiration; it is an instrument and a function to achieve excellence in quality of health care. Objective: To determine the level of occupational satisfaction of family doctors. Methods: A descriptive and cross-sectional study was carried out at Norte [North] Teaching Polyclinic of Placetas Municipality in 2019. The study population was made up of the 18 family doctors of the polyclinic, by nonprobabilistic sampling, casuistically deliberate based on the fact that there is no contact due to difficult access, the sample was made up of 15, with the willingness to participate as inclusion criterion. Results: Health services (93.3 percent) and privacy of the consultation (73.3 percent) predominated as unsuitable structural conditions; together with the office furniture and the waiting room (86.6 percent) as the so-called unsuitable occupational conditions; of the elements related to diagnosis and treatment, all were not adequate; of the subjective factors, the most referred to was stress (93.3 percent); and regarding degree of satisfaction, the category least satisfied (80 percent) predominated. Conclusions: There is little occupational satisfaction of the doctors of the Norte [North] Teaching Polyclinic of Placetas Municipality, determined by a set of interrelated factors(AU)

Complete Genome Sequence of Cluster A1 Mycobacterium smegmatis Bacteriophage Arlo.

Mycobacteriophage Arlo is a newly isolated Siphoviridae bacteriophage isolated from soil samples collected in Bluff Dale, Texas. Mycobacteriophage Arlo has a 52,960 base-pair double-stranded DNA genome that is predicted to contain 96 protein-coding genes. Mycobacteriophage Arlo is related to mycobacteriophage DD5 and other cluster A1 bacteriophages.

Dengue virus serotype-2 impairs proliferation of healthy donors' T lymphocytes.

OBJECTIVES: T lymphocytes are not infected by dengue virus (DENV), nevertheless it is possible that exposure to DENV may affect their function. T lymphocytes from DENV-infected individuals are impaired in their proliferative capacity, although this effect has been attributed to altered function of antigen-presenting cells rather than to an intrinsic defect on T lymphocytes. Here we analyzed whether T lymphocytes from healthy donors became impaired in their proliferative capacity following in vitro exposure to DENV serotype-2 (DENV-2), as well as the possible mechanisms for this. METHODS: Isolated CD4+ and CD8+ T lymphocytes from healthy donors were in vitro exposed to DENV-2, before polyclonal activation, cell proliferation, IL-2 synthesis. IL-2Rα expression, nuclear translocation of NF-AT and NF-κB, and intracellular calcium flux were assessed. RESULTS: In vitro exposure of both CD4+ and CD8+ T lymphocytes from healthy donors to DENV-2 impairs cell proliferation, IL-2 synthesis, and IL-2Rα (CD25) cell membrane expression. Signalling wise, exposure to DENV-2 impairs the nuclear translocation of NF-AT, downstream of intracellular calcium mobilization, as well as that of NF-κB. CONCLUSION: In the course of a dengue infection, direct exposure of T lymphocytes to DENV could affect cell-mediated immune responses.