RESUMO
Differentiation of marine luminous bacteria using Biology GN plate combined with API 20e or the BBL Crystal ID plate inoculated with cell suspensions in artificial seawater was accomplished by comparison to type species using cluster analysis. Inoculum density affected the results from Biolog GN plates, but had less of an effect on the reactions obtained from API 20e strip or BBL Crystal ID plate. In a few cases, combination of the Biolog GN traits along with either the API 20e or Crystal ID traits was necessary to differentiate some marine luminous bacteria.
Assuntos
Bactérias/classificação , Água do Mar/microbiologia , Bactérias/isolamento & purificação , Escherichia coli/classificação , Escherichia coli/isolamento & purificação , Bactérias Gram-Negativas/classificação , Bactérias Gram-Negativas/isolamento & purificação , Medições Luminescentes , Photobacterium/classificação , Photobacterium/isolamento & purificação , Filogenia , Vibrio/classificação , Vibrio/isolamento & purificaçãoRESUMO
Thirty-four strains of nonfermentative, respiratory, luminous bacteria were isolated from samples of squid ink and seawater from depths of 200 to 300 m in the Alboran Sea. Although these strains had a few properties similar to properties of Shewanella (Alteromonas) hanedai, they did not cluster phenotypically with any previously described bacterium. The nucleotide sequence of a 740-bp segment of luxA was not homologous with other known luxA sequences but clustered with the luxA sequences of Shewanella hanedai, Vibrio logei, Vibrio fischeri, and Photobacterium species. The 16S RNA gene from two strains was sequenced and was found to be most closely related to the S. hanedai 16S RNA gene. Based on the differences observed, we describe the new isolates as members of new species, Shewanella woodyi sp. nov. Strain ATCC 51908 (= MS32) is the type strain of this new species.
Assuntos
DNA Bacteriano/análise , Bacilos Gram-Negativos Anaeróbios Facultativos/classificação , Bacilos Gram-Negativos Anaeróbios Facultativos/genética , RNA Ribossômico 16S/análise , Microbiologia da Água , Composição de Bases , Ácidos Graxos/análise , Bacilos Gram-Negativos Anaeróbios Facultativos/química , Bacilos Gram-Negativos Anaeróbios Facultativos/fisiologia , Bacilos Gram-Negativos Anaeróbios Facultativos/ultraestrutura , Humanos , Medições Luminescentes , Filogenia , Reação em Cadeia da PolimeraseRESUMO
Luminous bacteria in seawater around the islands of Bahrain are predominantly Vibrio harveyi and have the capability to adhere to artificial fibrous surfaces. Phytoplankton did not appear to have any specific relationship with luminous bacteria, but macroalgae were shown to possess an enhanced concentration of luminous bacteria.
