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1.
J Exp Bot ; 69(9): 2379-2390, 2018 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-29190381

RESUMO

Branching has a major influence on the overall shape and productivity of a plant. Strigolactones (SLs) have been identified as plant hormones that have a key role in suppressing the outgrowth of axillary meristems. CAROTENOID CLEAVAGE DIOXYGENASE (CCD) genes are integral to the biosynthesis of SLs and are well characterized in annual plants, but their role in woody perennials is relatively unknown. We identified CCD7 and CCD8 orthologues from apple and demonstrated that MdCCD7 and MdCCD8 are able to complement the Arabidopsis branching mutants max3 and max4 respectively, indicating conserved function. RNAi lines of MdCCD7 show reduced gene expression and increased branching in apple. We performed reciprocal grafting experiments with combinations of MdCCD7 RNAi and wild-type 'Royal Gala' as rootstocks and scion. Unexpectedly, wild-type roots were unable to suppress branching in MdCCD7 RNAi scions. Another key finding was that MdCCD7 RNAi scions initiated phytomers at an increased rate relative to the wild type, resulting in a greater node number and primary shoot length. We suggest that localized SL biosynthesis in the shoot, rather than roots, controls axillary bud outgrowth and shoot growth rate in apple.


Assuntos
Dioxigenases/genética , Lactonas/metabolismo , Malus/genética , Reguladores de Crescimento de Plantas/metabolismo , Proteínas de Plantas/genética , Brotos de Planta/crescimento & desenvolvimento , Dioxigenases/metabolismo , Regulação da Expressão Gênica de Plantas , Malus/crescimento & desenvolvimento , Malus/metabolismo , Proteínas de Plantas/metabolismo , Brotos de Planta/genética
2.
Physiol Plant ; 154(1): 128-41, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25243478

RESUMO

The mature pigmented spathe of Zantedeschia is characterized by a developmental process, wherein the spathe regreens after anthesis and prior to senescence of the inflorescence. Previous research has shown that spathe regreening involves redifferentiation of chloroplasts and re-accumulation of chlorophyll, but the detailed physiological changes associated with regreening are still largely unknown. Using Zantedeschia aethiopica and the Zantedeschia pentlandii variety 'Best Gold' as models, this study explores the physiological mechanism and possible roles of fructification, 6-benzylaminopurine (BAP) and gibberellin (GA3 ) in induction or progression of spathe regreening. Application of BAP stimulated regreening in spathe tissue of 'Best Gold' by enhancing accumulation of carotenoid and chlorophyll, and also increasing stacking of grana. In contrast, GA3 retarded formation of double-membrane lamella during chloroplast redifferentiation, thus delaying the onset of regreening. We suggest that these actions of BAP and GA3 have a synergistic effect in delaying the onset of regreening in 'Best Gold' so that when applied together retardation of chlorophyll accumulation, chloroplast redifferentiation and accumulation of carotenoids were enhanced. The elimination of fructification did not prevent the occurrence of regreening in either Zantedeschia model plants, indicating that fructification was not a prerequisite for the induction of regreening. It is still unclear how regreening in Zantedeschia is triggered. We propose that the onset of regreening in Zantedeschia is likely to be a genetically programmed event.


Assuntos
Carotenoides/metabolismo , Clorofila/metabolismo , Topos Floridos/fisiologia , Zantedeschia/fisiologia , Compostos de Benzil , Topos Floridos/ultraestrutura , Giberelinas , Cinetina , Purinas , Zantedeschia/ultraestrutura
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