RESUMO
Light and immunofluorescence microscopies were used to study the cytopathic effect of herpes simplex virus type 1 (HSV-1) grown on the Vero cell cultures in the absence or presence of supernatants of Enterococcus faecium L3, Lactobacillus plantarum 8A-P3, and Escherichia coil M17. The effect of the probiotic strains was evaluated estimating the proportion of changed cells and the infective dose of the virus. The supernatants of the cultures of Lactobacillus sp. and Enterococcus sp., unlike those of E. coil, have antiviral activity. Inhibited viral replication was more evident when the supernatants were added until the cultured HSV-1 cells were infective. An enterococcal supernatant and its obtained peptide extract showed the maximum antiviral activity. This strain may be associated with the production of bacteriocins and bacteriocin-like substances.
Assuntos
Herpesvirus Humano 1/fisiologia , Lactobacillus plantarum/fisiologia , Probióticos , Replicação Viral , Animais , Bacteriocinas/biossíntese , Chlorocebus aethiops , Meios de Cultivo Condicionados/farmacologia , Efeito Citopatogênico Viral , Enterococcus faecium/virologia , Escherichia coli/fisiologia , Herpesvirus Humano 1/efeitos dos fármacos , Lactobacillus plantarum/metabolismo , Células VeroRESUMO
The population of circulating serotype 3 parainfluenza virus strains isolated in different years proved to be sufficiently polymorphic concerning its antigenic and biological features as well as their virulence for newborn hamsters. The highly virulent strain population appeared to have an antigenic pattern different from that of the prototype strain. The epidemic caused by it in groups of school and preschool children was more intensive as compared to that induced by avirulent strains population.
