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1.
J Pept Sci ; 24(7): e3089, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29808604

RESUMO

Cathelicidins are an important family of antimicrobial peptide effectors of innate immunity in vertebrates. Two members of this group, CATH-1 and CATH-2, have been identified and characterized in teleosts (ray-finned fish). In this study, we investigated the expression of these genes in different tissues of rainbow trout challenged with 4 different inactivated pathogens. By using qPCR, we detected a strong induction of both cath-1 and cath-2 genes within 24 hours after intraperitoneal inoculation with Lactococcus garvieae, Yersinia ruckeri, Aeromonas salmonicida, or Flavobacterium psychrophilum cells. Up to 700-fold induction of cath-2 was observed in the spleen of animals challenged with Y. ruckeri. Moreover, we found differences in the intensity and timing of gene up-regulation in the analyzed tissues. The overall results highlight the importance of cathelicidins in the immune response mechanisms of salmonids.


Assuntos
Aeromonas salmonicida/imunologia , Catelicidinas/imunologia , Flavobacterium/imunologia , Lactococcus/imunologia , Oncorhynchus mykiss/microbiologia , Yersinia ruckeri/imunologia , Aeromonas salmonicida/citologia , Animais , Antibacterianos/química , Antibacterianos/metabolismo , Catelicidinas/biossíntese , Catelicidinas/genética , Relação Dose-Resposta a Droga , Flavobacterium/citologia , Perfilação da Expressão Gênica , Lactococcus/citologia , Testes de Sensibilidade Microbiana , Oncorhynchus mykiss/genética , Oncorhynchus mykiss/imunologia , Baço/imunologia , Baço/microbiologia , Relação Estrutura-Atividade , Yersinia ruckeri/citologia
2.
Fish Shellfish Immunol ; 59: 456-468, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27818338

RESUMO

Cathelicidins, a major family of vertebrate antimicrobial peptides (AMPs), have a recognized role in the first line of defense against infections. They have been identified in several salmonid species, where the putative mature peptides are unusually long and rich in serine and glycine residues, often arranged in short multiple repeats (RLGGGS/RPGGGS) intercalated by hydrophobic motifs. Fragments of 24-40 residues, spanning specific motifs and conserved sequences in grayling or brown, rainbow and brook trout, were chemically synthesized and examined for antimicrobial activity against relevant Gram-positive and Gram-negative salmonid pathogens, as well as laboratory reference strains. They were not active in complete medium, but showed varying potency and activity spectra in diluted media. Bacterial membrane permeabilization also occurred only under these conditions and was indicated by rapid propidium iodide uptake in peptide-treated bacteria. However, circular dichroism analyses indicated that they did not significantly adopt ordered conformations in membrane-like environments. The peptides were not hemolytic or cytotoxic to trout cells, including freshly purified head kidney leukocytes (HKL) and the fibroblastic RTG-2 cell line. Notably, when exposed to them, HKL showed increased metabolic activity, while a growth-promoting effect was observed on RTG-2 cells, suggesting a functional interaction of salmonid cathelicidins with host cells similar to that shown by mammalian ones. The three most active peptides produced a dose-dependent increase in phagocytic uptake by HKL simultaneously stimulated with bacterial particles. The peptide STF(1-37), selected for further analyses, also enhanced phagocytic uptake in the presence of autologous serum, and increased intracellular killing of live E. coli. Furthermore, when tested on HKL in combination with the immunostimulant ß-glucan, it synergistically potentiated both phagocytic uptake and the respiratory burst response, activities that play a key role in fish immunity. Collectively, these data point to a role of salmonid cathelicidins as modulators of fish microbicidal mechanisms beyond a salt-sensitive antimicrobial activity, and encourage further studies also in view of potential applications in aquaculture.


Assuntos
Catelicidinas/genética , Catelicidinas/farmacologia , Salmonidae/imunologia , Sequência de Aminoácidos , Animais , Anti-Infecciosos/química , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Catelicidinas/química , Catelicidinas/isolamento & purificação , Proteínas de Peixes/química , Proteínas de Peixes/genética , Proteínas de Peixes/farmacologia , Domínios Proteicos , Salmonidae/genética , Salmonidae/microbiologia , Alinhamento de Sequência/veterinária
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