RESUMO
It has been observed that when Escherichia coli cells are treated simultaneously with phenanthroline and H2O2, there is a lethal interaction. In order to analyze the mechanism of this lethal interaction, wild-type and xthA mutant cells of E. coli were treated with 2.5 mM H2O2 and 1 mM phenanthroline. This treatment was preceded by treatments with different metal chelators (dipyridyl for Fe2+, desferal for Fe3+ and neocuproine for Cu2+) or conducted simultaneously to other treatments with chelators and radical scavengers (thiourea, ethanol and sodium benzoate). The lethal interaction was observed in both the E. coli wild-type strain and xthA mutant strain, which is deficient in the exonuclease III repair enzyme. Nevertheless, the mutant strain was much more sensitive than the wild-type one. Dipyridyl pretreatment protected the cells against the lethal interaction, while desferal pretreament was unable to do so. This suggests that the lethal interaction requires Fe2+ and not Fe3+ ions. Ethanol and sodium benzoate were incapable of protecting bacterial cells against the lethal interaction. Even a 20-min pretreatment with benzoate did not confer protection. On the other hand, thiourea protected the cells completely. Based on our results, we propose that the lethal interaction may be caused not only by the reaction kinetics of phenanthroline and Fe, but also by the ability of phenanthroline to intercalate in DNA. After forming the mono and bis complexes, phenanthroline would serve as a shuttle and take the Fe2+ ions to the DNA. So, the Fenton reaction would take its course with the consequent generation of OH. radicals near DNA. This proximity to the DNA would protect the OH. radicals against the scavengers' action, thus optimizing the Fenton reaction.
