[Effect of synthetic surfactants on some biological properties of non-pathogenic species of the genus Corynebacterium].

The influence of cationic, anionic and non-ionic synthetic surfactants on some biological properties of different representatives of the following species Corynebacterium glutamicum, Corynebacterium ammoniagenes, Corynebacterium vitaeruminis, Corynebacterium variabile and strain Corynebacterium sp. (Brevibacterium stationis) UKM As-719 has been investigated. It has been shown that the action of surfactants is accompanied by the change of cell morphology and loss of ability for Gram-staining, as well as by significant changes of antigenic properties and antibiotic-sensitivity of selected strains of corynebacteria. Nevertheless, no relations were established between the class of surfactants and changes in biological properties of the investigated strains. There was also no correlation between Gram-staining ability after the action of surfactants and change of corynebacteria antigenisity, as well as their antibiotic sensitivity.

[Serological affinity of some species of nonpathogenic corynebacteria]. / Serologichna sporidnenist' deiakikh vydiv nepatohennykh korynebakterii.

Serological peculiarities of the species strains Corynebacterium glutamicum, C. ammoniagenes, C. vitaeruminis, C. variabilis and strain of Corynebacterium sp. (Brevibacterium stationis) UCM Ac-719 have been investigated with the help of immunoenzyme analysis ELISA with the use of mice immune serum, specific to C. ammoniagenes UCM Ac-732T, C. vitaeruminis UCM Ac-718T, C. variabilis UCM Ac-717T, C. glutamicum UCM Ac-733 and Corynebacterium sp. UCM Ac-719. It has been established that the species of nonpathogenic corynebacteria differ between themselves as to the degree of serological affinity. C. variabilis, C. ammoniagenes and C. glutamicum are the least similar as to this indication. Weak antigenic relations have been revealed in C. vitaeruminis and C. ammoniagenes. The latter displayed the higher, as compared with other strains, affinity for Corynebacterium sp. UCM Ac-719. The highest degree of serological affinity within the species was registered in strains C. glutamicum and C. variabilis. Data obtained evidence that the ELISA method permits conducting the high-reliability species diagnosis of nonpathogenic corynebacteria on the basis of their antigenic characteristics.

[Electrophoretic patterns of cell wall protein as a criterion for the identification and classification of Corynebacteria]. / Elektroforetychni spektry bilkiv klitynnoï stinky iak odyn iz kryteriïv identyfikatsiï ta klasyfikatsiï korynebakterii.

Electrophoretic patterns of cell wall protein of three industrial strains, that were used for production of lysin, and eight collection strains from the genus Corynevacterium were studied to analyze their similarity as well as to estimate an opportunity of using this parameter as an additional criterion for identification and classification of corynebacteria. Similarity coefficient of cell wall overall and main protein electrophoretic patterns were determined by a specially created computer program. Electrophoretic analysis showed that every specie had an individual protein profile. There were determined biopolymers common for the specie, genus and individual among the overall majors and minors. The obtained results showed, that the patterns of main proteins were more conservative and informative in comparison with those ones of overall proteins. The definition of similarity coefficient by the main protein patterns has correlated with the protein profile characteristics of every analyzed strain, and it managed to distribute them into the separate groups. The similarity coefficient of preparations by the main protein patterns allows to separate one specie or a strain from another, and that gives us a chance to claim that this parameter could be used as an additional criterion for differentiation and referring the corynebacteria to a certain taxonomic group.

[The serological properties of saprophytic corynebacteria studied by immunoenzyme analysis]. / Issledovanie serologicheskikh svoistv saprofitnykh korinebakterii s pomoshch'iu immunofermentnogo analiza.

The degree of serological similarity of saprophytic corynebacteria have been studied using immunoassay ELISA analysis, that is seven collection strains, belonging to Corynebacterium glutamicum (3 strains), C. ammoniagenes (1 strain), C. vitarumen (1 strain), C. variabilis (2 strains) and three industrial strains-lysine producers. Intact and heated bacteria cells have been used as antigens. It has been shown that industrial strain C. glutamicum 22L and collection strains C. glutamicum IMV AC-715, IMV AC-714, IMV AC-733 have the highest degree of serological relationship. C. vitarumen IMV AC-718, C variabilis IMV AC-716 as well as Corynebacterium sp. E531 and VNIIgenetics 90 are close to them according to their serological properties. C. ammoniagenes IMV AC-732 and C. variabilis IMV AC-717 strains have the lowest degree of similarity with other saprophytic corynebacteria which have been studied.

[The serological properties of a lysine producer developing in a batch culture]. / Serologicheskie svoistva produtsenta lizina, razvivaiushchegosia v periodicheskoi kul'ture.

Immune sera specific to bacterial cells at various development stages of periodic culture have been obtained with the purpose to study serological properties of lysin producer. Using the method of immunoenzyme analysis ELISA it is shown that there is certain dependence between the producer properties and the culture age. Different strains-producers used in production to-day are serologically close between each other. When using 49 strains-contaminants of lysin production (representatives of 10 genera) the absence of serological affinity between the producer and foreign microflora has been demonstrated. Preliminary data prove the prevalence of cells producers of thermostable antigens on the cell surface.

[Gram-negative bacteria contaminating the process of producing lysine]. / Gramotritsatel'nye bakterii, kontaminiruiushchie protsess proizvodstva lizina.

The authors have isolated Gram-negative bacteria of the Enterobacteriaceae family from the culture liquid of industrial fermenters with low yield of lysine. Most of them possessed the characters typical of Klebsiella pneumoniae and Escherichia coli, the rest were identified as the representatives of genera Proteus, Providencia, Enterobacter, Hafnia. Strains of Klebsiella pneumoniae, E.coli, Proteus rettgeri manifested lysine-decarboxylase activity. The capacity of some strains to destruct lysine synthesized by the target culture in the process of fermentation with formation of cadaverin was experimentally proved and confirmed under production conditions. Technological water is the source of distribution of gram-negative bacteria (first of all Klebsiella) in lysine production.

[The immunosuppressive activity of Streptococcus aureus peptidoglycan]. / Immunosupressivnaia aktivnost' peptidoglikana Staphylococcus aureus.

The immunosuppressive activity of peptidoglycan, obtained from S. aureus was studied in mouse experiments on the model of delayed hypersensitivity (DH) to guinea pig antigens. The study revealed that PG suppressed DH, this action being linked with its insoluble fraction. The soluble part of PG did not suppress DH. Experiments with the use of immunosorbent demonstrated the existence of serological similarity between the active factor of the soluble part of PG and its insoluble part.

[The inhibition of the cellular immune response by Pseudomonas aeruginosa extracts]. / Ingibirovanie kletochnogo immunnogo otveta ékstraktami sinegnoinoi palochki.

Aquatic-phenol trichloroacetic and salt extracts of Pseudomonas aeruginosa have been studied for their effect on hypersensitivity of the delayed type to guinea pig antigen in mice. It is found that salt extracts of wild strains contain thermostable immunosuppressive factors which are of the lipid nature, have high molecular weight and can be inactivated by trichloroacetic acid and phenol. The salt extracts of museum strains as well as aquatic-phenol and trichloroacetic extracts obtained from wild and museum strains had no immunosuppressive activity.

[The diagnostic importance of antibodies to the cell wall peptidoglycan of Staphylococcus in the blood serum of children with a staphylococcal infection]. / Diagnosticheskoe znachenie antitel k peptidoglikanu kletochnoi stenki stafilokokka v syvorotke krovi detei so stafilokokkovoi infektsiei.

High titers of antibodies to peptidoglycan of staphylococcus aureus were found in children with purulent-inflammatory diseases of staphylococcal etiology. This may be used for the diagnosis of staphylococcal infection.