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1.
Heliyon ; 10(7): e28734, 2024 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-38617959

RESUMO

Rationale and objectives: Imaging plays a key role in Slipped Capital Femoral Epiphysis diagnosis and severity assessment. In the last two decades, signs and measurements emerged in literature showed potential to help in SCFE diagnosis and tailoring treatment. The purpose of this review is to collect and discuss new imaging signs, measurements, and techniques according to investigations published after 2000 to improve SCFE diagnosis. Material and methods: The PubMed, Scopus, and Science Direct databases were used to search for relevant articles related to imaging in SCFE diagnosis from January 2000 to March 2023. Article selection and review was performed by two board-certified radiologists). Article quality assessment were conducted by authors using QUADAS-2 and SANRA evaluation tools. Results: The research resulted in a total of 2577 articles. After duplicates removal and abstract analysis, 28 articles were finally selected for full-text analysis. Seventeen articles were focused on Radiographs, 6 on CT, 1 on both Radiographs and CT, 4 on MRI. No study focused on ultrasound was selected. Conclusions: Use of modified Klein's line and S-sign may improve radiographs accuracy in daily routine. Lucency sign may help in early diagnosis on radiographs. Preoperative CT may be useful in planning a tailored treatment predicting SCFE severity and instability. MRI is the most accurate modality to diagnose SCFE at early stage. Nevertheless, it cannot be used to predict the risk of contralateral SCFE. Risk prediction can be assessed with radiographs, using a new rapid mOBS. Further investigation and validation of these sign is needed.

2.
Eur J Med Genet ; 68: 104926, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38369057

RESUMO

Osteogenesis imperfecta (OI) is a rare phenotypically and genetically heterogeneous group of inherited skeletal dysplasias. The hallmark features of OI include bone fragility and susceptibility to fractures, bone deformity, and diminished growth, along with a plethora of associated secondary features (both skeletal and extraskeletal). The diagnosis of OI is currently made on clinical grounds and may be confirmed by genetic testing. However, imaging remains pivotal in the evaluation of this disease. The aim of this article is to review the current role played by the various radiologic techniques in the diagnosis and monitoring of OI in the postnatal setting as well as to discuss recent advances and future perspectives in OI imaging. Conventional Radiography and Dual-energy X-ray Absorptiometry (DXA) are currently the two most used imaging modalities in OI. The cardinal radiographic features of OI include generalized osteopenia/osteoporosis, bone deformities, and fractures. DXA is currently the most available technique to assess Bone Mineral Density (BMD), specifically areal BMD (aBMD). However, DXA has important limitations and cannot fully characterize bone fragility in OI based on aBMD. Novel DXA-derived parameters, such as Trabecular Bone Score (TBS), may provide further insight into skeletal changes induced by OI, but evidence is still limited. Techniques like Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) can be useful as problem-solvers or in specific settings, including the evaluation of cranio-cervical abnormalities. Recent evidence supports the use of High-Resolution peripheral Quantitative Computed Tomography (HR-pQCT) as a promising tool to improve the characterization of bone fragility in OI. However, HR-pQCT remains a primarily research technique at present. Quantitative Computed Tomography (QCT) is an alternative to DXA for the determination of BMD at central sites, with distinct advantages but considerably higher radiation exposure. Quantitative Ultrasound (QUS) is a portable, inexpensive, and radiation-free modality that may complement DXA evaluation, providing information on bone quality. However, evidence of usefulness of QUS in OI is poor. Radiofrequency Echographic Multi Spectrometry (REMS) is an emerging non-ionizing imaging method that holds promise for the diagnosis of low BMD and for the prediction of fracture risk, but so far only one published study has investigated its role in OI. To conclude, several different radiologic techniques have proven to be effective in the diagnosis and monitoring of OI, each with their own specificities and peculiarities. Clinicians should be aware of the strategic role of the various modalities in the different phases of the patient care process. In this scenario, the development of international guidelines including recommendations on the role of imaging in the diagnosis and monitoring of OI, accompanied by continuous active research in the field, could significantly improve the standardization of patient care.


Assuntos
Fraturas Ósseas , Osteogênese Imperfeita , Osteoporose , Humanos , Osteogênese Imperfeita/diagnóstico por imagem , Osteogênese Imperfeita/genética , Osteogênese Imperfeita/patologia , Densidade Óssea , Absorciometria de Fóton/métodos , Fraturas Ósseas/diagnóstico por imagem
3.
Sanid. mil ; 78(4): 273-278, Oct-Dic. 2022. ilus
Artigo em Espanhol | IBECS | ID: ibc-220570

RESUMO

La acumulación de aire y/o líquido en el espacio pleural conlleva un incremento de la morbimortalidad. El drenaje pleural permite la evacuación del contenido anormal de la cavidad pleural, con la consiguiente reexpansión pulmonar y estabilización cardiorrespiratoria. La complejidad cada vez mayor de los enfermos ingresados en el hospital hace frecuente en la práctica clínica la necesidad de colocación de un drenaje torácico. Tanto la colocación como los cuidados deben ser realizados por personal entrenado. La presente revisión no tiene como objetivo describir la patología pleural sino demostrar de una forma didáctica y práctica las pautas básicas para la colocación y el manejo adecuado de los sistemas de drenaje pleural al personal sanitario para contribuir a mejorar la seguridad de la práctica clínica. Entre los mayores avances destacan la introducción de la ecografía torácica y la utilización de nuevas técnicas de drenaje.(AU)


Accumulation of air and/or liquid in the pleural cavity leads to an increase in morbidity and mortality. Chest drainage allows the evacuation of the abnormal content of the pleural cavity, with the consequent lung expansion and cardiorespiratory stability. The increased patient-related complexity in hospitals often carries to place chest drainage in clinical practice. Both placement and care must be performed by a highly trained staff. The objective of this review is not to describe pleural pathology, but to demonstrate in a didactic and practical way the basic guidelines for placement and management of chest drainage systems for healthcare professionals to improve workplace safety. Among the greatest advances are the introduction of chest ultrasound and the use of new drainage techniques.(AU)


Assuntos
Humanos , Derrame Pleural , Drenagem , Pneumotórax , Tórax/diagnóstico por imagem
4.
Int J Tuberc Lung Dis ; 26(9): 862-868, 2022 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-35996291

RESUMO

OBJECTIVE: To evaluate the diagnostic accuracy of Xpert® MTB/RIF Ultra (Ultra) on fresh respiratory samples for the diagnosis of pulmonary TB (PTB) in children.METHODS: Between July 2017 and December 2019, children with presumed TB were prospectively enrolled at clinical sites in three African countries. Children were assessed using history, physical examination and chest X-ray. Sputum or gastric aspirate samples were analysed using Ultra and culture. The diagnostic accuracy of Ultra was calculated against culture as the reference standard.RESULTS: In total, 547children were included. The median age was 4.7 years, 77 (14.1%) were HIV infected and 77 (14.1%) had bacteriologically confirmed TB. Ultra detected an additional 20 cases in the group of children with negative culture results. The sensitivity of Ultra was 66.3% (95% CI 47-82), and the specificity was 95.4% (95% CI 89-99) when assessed against culture as the reference standard.CONCLUSION: Despite the improved performance of Ultra as compared to Xpert as was previously reported, its sensitivity remains sub-optimal for the detection of TB in children. Ultra detected additional 20 cases which otherwise could not have been detected by culture alone, suggesting that the latter is an imperfect reference standard.


Assuntos
Mycobacterium tuberculosis , Tuberculose Pulmonar , Criança , Pré-Escolar , Farmacorresistência Bacteriana , Humanos , Rifampina , Sensibilidade e Especificidade , Escarro , Tuberculose Pulmonar/diagnóstico
5.
Rev Esp Quimioter ; 35(5): 482-491, 2022 Oct.
Artigo em Espanhol | MEDLINE | ID: mdl-35841598

RESUMO

OBJECTIVE: Congenital cytomegalovirus infection (cCMV) has been considered more prevalent among HIV-exposed children during pregnancy. Spanish national guidelines recommend the cCMV screening in these newborns. Nowadays, pregnant women have a better control of HIV infection compared to previous decades. We aim to analyze the prevalence and associated risk factors to cCMV in these children. METHODS: A retrospective cross-sectorial study was performed. All newborns exposed to HIV were assisted in a third-level hospital (2014-2020). Epidemiological and clinical data of the mother and newborn were recorded. Shell vial urine culture and/or CRP were performed along the two first weeks of life for the neonatal screening of cCMV. RESULTS: Overall 69 newborns were enrolled. A high proportion (82.4%) of the mothers had been diagnosed with HIV before getting pregnant. All women received ART during the pregnancy. Median T-CD4 lymphocytes before delivery was 641/mm3 (IQR: 480-865) and the viral load was undetectable in 83.6%. Serological test for CMV along the first trimester of pregnancy was performed in 73.5% (positive IgG in 96%). There were no congenital cases of HIV neither cCMV (CI 95%:0-5.3%). CONCLUSIONS: The cCMV prevalence in newborns exposed to HIV was 0%, lower than reported before, probably related to a better and earlier ART during pregnancy, leading to a better immunological status.


Assuntos
Infecções por Citomegalovirus , Infecções por HIV , Criança , Citomegalovirus/genética , Infecções por Citomegalovirus/complicações , Infecções por Citomegalovirus/diagnóstico , Infecções por Citomegalovirus/epidemiologia , DNA Viral , Feminino , Infecções por HIV/complicações , Infecções por HIV/epidemiologia , Humanos , Imunoglobulina G , Recém-Nascido , Gravidez , Estudos Retrospectivos
6.
Rev Esp Quimioter ; 35(3): 284-287, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35355046

RESUMO

OBJECTIVE: Bloodstream infections (BSI) caused by extended-spectrum beta-lactamases Enterobacteriaceae (ESBL-E) are associated with high rates of treatment failure and increased mortality, especially when appropriate antimicrobial therapy is delayed. Our aim was to evaluate the anticipation of ESBLs detection and the potential improvement of the time response of the Vitek 2 System (BioMérieux; France). METHODS: We compared this lateral flow immunoassay when used directly on fluid from positive blood cultures to the VITEK2 AST system. We evaluated 80 isolates, 61 tested directly on fluid from positive blood cultures and 19 tested on fluid from blood cultures spiked with known ESBL positive and negative organisms. RESULTS: The concordance between the CTX-LFIA and the reference method (Vitek 2) had a Cohen´s Kappa coefficient of 0.97, indicating a particularly good correlation between both compared methods. CONCLUSIONS: This lateral flow immunoassay can be more rapid than the Vitek 2 for earlier presumptive identification of CTX-M ESBLs and can be useful to anticipate results and the adjustment of antimicrobial therapy.


Assuntos
Gestão de Antimicrobianos , Infecções por Enterobacteriaceae , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Hemocultura , Infecções por Enterobacteriaceae/tratamento farmacológico , Humanos , Imunoensaio , Testes de Sensibilidade Microbiana , beta-Lactamases
7.
J Antimicrob Chemother ; 76(1): 220-225, 2021 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-33038895

RESUMO

BACKGROUND: Carbapenem-resistant Enterobacteriaceae (CRE) are an emerging problem in the paediatric population worldwide with high mortality rates in bloodstream infection (BSI). OBJECTIVES: To evaluate predictors of 30 day mortality in CRE BSI in a paediatric cohort. METHODS: A retrospective observational single-centre study (December 2005-August 2018) was conducted. Cases of CRE BSI in children 0 to 16 years were included. Microbiological identification (MALDI Biotyper) and antimicrobial susceptibility testing (Vitek2® and MicroScan panel NBC44) according to EUCAST breakpoints were performed. PCR OXVIKP® was used to confirm carbapenemase genes (OXA-48, VIM, KPC, NDM). Demographic characteristics, underlying diseases, source of bacteraemia, antimicrobial therapy and outcomes were collected from medical records. Survival analysis to establish predictors of 30 day mortality was performed. RESULTS: Thirty-eight cases were included; 76.3% were hospital-acquired infections and 23.7% related to healthcare. All patients had at least one underlying comorbidity and 52.6% were recipients of an organ transplant. VIM carbapenemase was the predominant mechanism (92.1%). Previous CRE colonization or infection rate was 52.6%. Intestinal tract (26.3%) and vascular catheter (21.1%) were the most common sources of infection. Crude mortality within 30 days was 18.4% (7/38); directly related 30 day mortality was 10.5%. Conditions associated with an increment in 30 day mortality were intensive care admission and inadequate empirical therapy (P < 0.05). Combination-antibiotic targeted treatment and a low meropenem MIC were not related to improved survival. CONCLUSIONS: CRE BSI mortality rate is high. The most important factor related to 30 day survival in our CRE BSI cohort in children was empirical treatment that included at least one active antibiotic.


Assuntos
Enterobacteriáceas Resistentes a Carbapenêmicos , Infecções por Enterobacteriaceae , Sepse , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Proteínas de Bactérias/genética , Carbapenêmicos/farmacologia , Criança , Infecções por Enterobacteriaceae/tratamento farmacológico , Infecções por Enterobacteriaceae/epidemiologia , Humanos , Estudos Retrospectivos , Sepse/tratamento farmacológico , beta-Lactamases/genética
8.
Eur J Clin Microbiol Infect Dis ; 40(4): 779-785, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33083918

RESUMO

Herbaspirillum species are Gram-negative bacteria belonging to the class Betaproteobacteria, order Burkholderiales. The phylogenetic and phenotypic similarities among these groups easily lead to species misidentification. Herbaspirillum bacteraemia is an uncommon clinical entity. The objective of this review is to collect information to contribute to the management of this infection. We describe our own case series and review the cases reported in the literature. Cancer appears as the major underlying disease. The main source of bacteraemia was respiratory. Phenotypic identification methods often misidentify this species. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and molecular methods identify at genus level, but species assignment is not reliable. Herbaspirillum spp. showed a highly susceptible antimicrobial profile. ß-Lactams showed good activity with low MIC values, except ampicillin. All isolates were resistant to colistin, suggesting an intrinsic resistance mechanism. Herbaspirillum spp. is an uncommon pathogen. MALDI-TOF MS or molecular methods are necessary to achieve a reliable genus identification. These species are not multidrug resistant. Piperacillin/tazobactam or ceftazidime might be a good treatment for this microorganism.


Assuntos
Bacteriemia/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Herbaspirillum/isolamento & purificação , Adulto , Idoso , Infecções por Bactérias Gram-Negativas/sangue , Humanos , Lactente , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade
9.
Dis Aquat Organ ; 141: 127-138, 2020 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-32969345

RESUMO

Caligus rogercresseyi is the dominant sea louse parasite affecting the salmon and trout industry in southern Chile. This parasite has a wide range of native and endemic fish hosts. The Patagonian blenny Eleginops maclovinus, which is parasitized mostly by the caligid species Lepeophtheirus spp. and C. rogercresseyi, is presumably responsible for the transmission of C. rogercresseyi to salmonids. The aim of this study was to characterize the transmission of parasites between different fish species and parasite cohort development under laboratory conditions. Parasite abundances and intensities were quantified. Transmission of parasites from Patagonian blenny to Atlantic salmon Salmo salar was lower (~9%, mainly corresponding to C. rogercresseyi) than from salmon to Patagonian blenny (14.7-26.9%, where only C. rogercresseyi were observed). This suggests that the transmission of C. rogercresseyi from salmon individuals is higher than the transmission from a native fish. Parasite cohorts developed successfully on both fish species, but apparently under different developmental rates. Water temperature, oxygen, and juvenile abundances were the variables that better explained cohort development success and variation in C. rogercresseyi adult abundances over time.


Assuntos
Copépodes , Doenças dos Peixes , Salmo salar , Salmonidae , Animais , Chile/epidemiologia , Doenças dos Peixes/epidemiologia , Salmão
11.
BMC Infect Dis ; 20(1): 469, 2020 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-32615981

RESUMO

BACKGROUND: Interferon-γ release assays (IGRA) with Resuscitation promoting factor (Rpf) proteins enhanced tuberculosis (TB) screening and diagnosis in adults but have not been evaluated in children. Children often develop paucibacillary TB and their immune response differs from that of adults, which together affect TB disease diagnostics and immunodiagnostics. We assessed the ability of Rpf to identify infection among household TB-exposed children in The Gambia and investigated their ability to discriminate Mycobacterium tuberculosis complex (MTBC) infection from active TB disease in children. METHODS: Detailed clinical investigations were done on 93 household TB-exposed Gambian children and a tuberculin skin test (TST) was administered to asymptomatic children. Venous blood was collected for overnight stimulation with ESAT-6/CFP-10-fusion protein (EC), purified protein derivative and RpfA, B, C, D and E. Interferon gamma (IFN-γ) production was measured by ELISA in supernatants and corrected for the background level. Infection status was defined by IGRA with EC and TB disease by mycobacterial confirmation and/or clinical diagnosis. We compared IFN-γ levels between infected and uninfected children and between infected and TB diseased children using a binomial logistic regression model while correcting for age and sex. A Receiver Operating Characteristics analysis was done to find the best cut-off for IFN-γ level and calculate sensitivity and specificity. RESULTS: Interferon gamma production was significantly higher in infected (IGRA+, n = 45) than in uninfected (IGRA-, n = 20) children after stimulation with RpfA, B, C, and D (P = 0.03; 0.007; 0.03 and 0.003, respectively). Using RpfB and D-specific IFN-γ cut-offs (33.9 pg/mL and 67.0 pg/mL), infection was classified with a sensitivity-specificity combination of 73-92% and 77-72% respectively, which was similar to and better than 65-75% for TST. Moreover, IFN-γ production was higher in infected than in TB diseased children (n = 28, 5 bacteriologically confirmed, 23 clinically diagnosed), following RpfB and D stimulation (P = 0.02 and 0.03, respectively). CONCLUSION: RpfB and RpfD show promising results for childhood MTBC infection screening, and both performed similar to and better than the TST in our study population. Additionally, both antigens appear to discriminate between infection and disease in children and thus warrant further investigation as screening and diagnostic antigens for childhood TB.


Assuntos
Antígenos de Bactérias/imunologia , Proteínas de Bactérias/imunologia , Citocinas/imunologia , Testes de Liberação de Interferon-gama/métodos , Tuberculose Latente/diagnóstico , Tuberculose Latente/epidemiologia , Programas de Rastreamento/métodos , Mycobacterium tuberculosis/imunologia , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Características da Família , Feminino , Gâmbia/epidemiologia , Humanos , Interferon gama/imunologia , Interferon gama/metabolismo , Tuberculose Latente/microbiologia , Masculino , Sensibilidade e Especificidade , Teste Tuberculínico
13.
Transplant Proc ; 50(2): 374-381, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29579807

RESUMO

BACKGROUND: In 2011, the European Directorate for the Quality of Medicines & Healthcare of the Council of Europe launched a 3-year collaborative project to address the organ shortage and improve access to transplant health services in Council of Europe member states in the Black Sea area (Armenia, Azerbaijan, Bulgaria, Georgia, Moldova, Romania, Turkey, Ukraine, and the Russian Federation) through the development of safe and ethical donation and transplantation programs. OBJECTIVE: Support the development of donation and transplantation programs through close interstate cooperation between national health organizations and relevant stakeholders. METHODOLOGY: Several work packages (WP) were established: WP1, project coordination (European Directorate for the Quality of Medicines & Healthcare); WP2, development and implementation of an effective legislative and financial framework (Czech Republic and France); WP3, establishment of National Transplant Authorities (Italy and Portugal); and WP4, clinical practices (DTI Foundation). Data collection, surveys, and expert visits allowed for the collection of first-hand information from each participant country at national, regional, and hospital levels. RESULTS: Data analysis showed the positive impact of the project represented by a tendency to increase the total donation rates (per million people) in the participant countries (2011 vs 2013): Azerbaijan, +7.3; Armenia, -0.7; Georgia, +3.3; Bulgaria, +0.9; Moldova, +2.5; Ukraine:, +0.8; Romania, +2.3; and Turkey, +2.7. CONCLUSIONS: Increases in total donation rates are the result of a number of initiatives in the Black Sea area, including the stepwise implementation of legislative, organizational and institutional country-specific recommendations tailored by the CoE, efforts of the respective Ministries of Health in each country and synergism with other European projects in the region. These countries should invest further in implementing the recommendations that emerged from this project to improve their organ donation and transplantation programs and progress toward self-sufficiency.


Assuntos
Cooperação Internacional , Obtenção de Tecidos e Órgãos/organização & administração , Transplantes/provisão & distribuição , Mar Negro , França , Humanos , Itália , Moldávia , Portugal , Romênia , Turquia
14.
Eur J Clin Microbiol Infect Dis ; 36(12): 2469-2473, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28831593

RESUMO

We have performed a retrospective, before-after comparison of turnaround time and therapy adjustment parameters before and after the introduction of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) plus mecA polymerase chain reaction (PCR) for the identification of methicillin-resistant Staphylococcus aureus (MRSA) in positive blood cultures. There were 227 episodes of S. aureus bacteremia during the study periods. The pre-MALDI-TOF and post-MALDI-TOF groups included 133 and 94 patients, respectively. The two rapid methods performed sequentially decreased the turnaround time of MRSA identification by nearly 50% (2.06 ± 1.95 vs. 3.95 ± 1.70 days). There was no significant reduction in the length of hospitalization (28.27 ± 32.16 vs. 28.62 ± 28.75 days). In both groups, the adequacy of the empirical antibacterial therapy was similar (59.49% vs. 51.31%), but the optimization of the therapy was more frequent in the post-MALDI-TOF group. Routine implementation of these techniques provides results earlier than conventional methods and increases the proportion of episodes with adequate change of empirical to directed antimicrobial therapy.


Assuntos
Bacteriemia , Infecções Estafilocócicas/diagnóstico , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus , Adulto , Idoso , Idoso de 80 Anos ou mais , Técnicas Bacteriológicas , Hemocultura , Feminino , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase/métodos , Estudos Retrospectivos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Infecções Estafilocócicas/terapia , Staphylococcus aureus/classificação , Staphylococcus aureus/genética
15.
J Fish Dis ; 39(7): 853-65, 2016 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-26644318

RESUMO

This study looks at how low infestation loads of adult Caligus rogercresseyi and other stressors affect the physiology of Salmo salar. Experimental fish groups were with (infested) or without (control) exposure to the parasite. The parasite cohort was followed for 78 days post-infestation (dpi), and only adult lice were observed. Additional stressors were applied at 60 and 75 dpi. The analysis included measurements of fish physiology and weight. Low-level infestations by adult C. rogercresseyi for more than 50 dpi induced moderate stress in S. salar as well as a high energy demand and increased small skin mucous cells. Threshold lice loads were identified, and above those loads, a high stress response was observed. Additional stressors altered fish physiology, inducing downregulation of the cortisol response after the first stressor and upregulation after the second stressor, but infested fish responded more strongly. Parasitism by C. rogercresseyi is energetically demanding, affecting the primary and secondary responses (e.g. cortisol and glucose levels), as well as the tertiary response (fish weight).


Assuntos
Copépodes/fisiologia , Ectoparasitoses/veterinária , Doenças dos Peixes/fisiopatologia , Hidrocortisona/sangue , Salmo salar , Estresse Fisiológico , Animais , Peso Corporal , Regulação para Baixo , Ectoparasitoses/fisiopatologia , Feminino , Doenças dos Peixes/parasitologia , Hidrocortisona/genética , Masculino , Densidade Demográfica , Distribuição Aleatória
16.
Transplant Proc ; 47(8): 2328-31, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26518918

RESUMO

BACKGROUND: The SEUSA program, the Donation and Transplantation Institute foundation consultancy program, was implemented in Trinidad and Tobago (T&T) in 2010 with the support of the National Organ Transplant Unit (NOTU) and the Ministry of Health of T&T. METHODS: The SEUSA program included (1) diagnosis of the current situation using the ODDS (Organ Donation Diagnostic Surveys); (2) creation of a human resources structure through Transplant Procurement Management (TPM); (3) detection of all brain and cardiac deaths in the hospitals implementing the DAS (Decease Alert System); (4) in-hospital awareness based on the EODS (Essentials in Organ Donation); and (5) external hospital audits. Additionally continued monitoring is performed. RESULTS: Thus far, thanks to implementation of the SEUSA program in Trinidad and Tobago 175, healthcare professionals have been exposed to training programs in the organ donation field. The Living Kidney Program was reinforced and the structure of the Deceased Donation (DD) network was defined. Since 2010, 485 potential organ donors have been detected, and 9 have become actual organ donors; 74 patients have received a kidney transplant (59 from living and 15 from deceased donors). CONCLUSIONS: This project results demonstrate that the application of the SEUSA program is an efficient methodology to develop DD programs that increase and consolidate transplant programs in the Caribbean region.


Assuntos
Desenvolvimento de Programas , Obtenção de Tecidos e Órgãos/organização & administração , Humanos , Transplante de Órgãos/estatística & dados numéricos , Inquéritos e Questionários , Doadores de Tecidos/estatística & dados numéricos , Trinidad e Tobago
17.
J Dairy Sci ; 97(11): 6719-28, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25200790

RESUMO

Although milk polar lipids such as phospholipids and sphingolipids located in the milk fat globule membrane constitute 0.1 to 1% of the total milk fat, those lipid fractions are gaining increasing interest because of their potential beneficial effects on human health and technological properties. In this context, the accurate quantification of the milk polar lipids is crucial for comparison of different milk species, products, or dairy treatments. Although the official International Organization for Standardization-International Dairy Federation method for milk lipid extraction gives satisfactory results for neutral lipids, it has important disadvantages in terms of polar lipid losses. Other methods using mixtures of solvents such as chloroform:methanol are highly efficient for extracting polar lipids but are also associated with low sample throughput, long time, and large solvent consumption. As an alternative, we have optimized the milk fat extraction yield by using a pressurized liquid extraction (PLE) method at different temperatures and times in comparison with those traditional lipid extraction procedures using 2:1 chloroform:methanol as a mixture of solvents. Comparison of classical extraction methods with the developed PLE procedure were carried out using raw whole milk from different species (cows, ewes, and goats) and considering fat yield, fatty acid methyl ester composition, triacylglyceride species, cholesterol content, and lipid class compositions, with special attention to polar lipids such as phospholipids and sphingolipids. The developed PLE procedure was validated for milk fat extraction and the results show that this method performs a complete or close to complete extraction of all lipid classes and in less time than the official and Folch methods. In conclusion, the PLE method optimized in this study could be an alternative to carry out milk fat extraction as a routine method.


Assuntos
Bovinos , Cromatografia Líquida de Alta Pressão/métodos , Cabras , Lipídeos/análise , Leite/química , Ovinos , Animais , Colesterol/análise , Ácidos Graxos/análise , Feminino , Glicolipídeos/química , Glicoproteínas/química , Humanos , Gotículas Lipídicas , Lipídeos/isolamento & purificação , Óvulo , Fosfolipídeos/análise , Esfingolipídeos/análise , Triglicerídeos/análise
18.
Transplant Proc ; 46(4): 1044-8, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24815123

RESUMO

OBJECTIVE: The International Registry in Organ Donation and Transplantation (IRODaT) presents final data on worldwide donation and transplantation activity to the community. The purpose of this report is not only to present raw data, but also to make organ donation effectiveness rates a useful tool to reveal similarities between countries on an international level. MATERIALS AND METHODS: The IRODaT Registry produced this report of 2013 statistics thanks to the collaboration of the official reporters, professionals in coordination and transplant services from national transplant organizations, and from the Ministries of Health of each reported country. RESULTS: The actual deceased donors and living donors rates, according to the total number of donors, from 72 countries can be analyzed using IRODaT tools, allowing the comparison and study of tendencies and rates of organ donation and transplantation activity at an international scale. IRODaT has been collecting and disseminating worldwide data since 1998. In this issue, it is shown that most of the countries that report the major activity in actual deceased donors are located in the European region. On the other hand, regarding living organ donation, the country location is more varied, including countries from Africa, Asia, and America. CONCLUSIONS: The results obtained through the IRODaT Registry provide a quick, clear, and illustrative view of worldwide organ donation activity.


Assuntos
Saúde Global/estatística & dados numéricos , Transplante de Órgãos/estatística & dados numéricos , Sistema de Registros/estatística & dados numéricos , Doadores de Tecidos/estatística & dados numéricos , Obtenção de Tecidos e Órgãos/estatística & dados numéricos , Comportamento Cooperativo , Humanos , Disseminação de Informação , Cooperação Internacional , Doadores Vivos/estatística & dados numéricos , Doadores de Tecidos/provisão & distribuição
19.
Radiología (Madr., Ed. impr.) ; 56(1): 52-60, ene.-feb. 2014. ilus
Artigo em Espanhol | IBECS | ID: ibc-118520

RESUMO

A pesar de que el diagnóstico inicial de la enfermedad pleuro-pulmonar y el seguimiento habitual de los pacientes se hace con una radiografía simple de tórax, y que el estándar de referencia para estudiar la enfermedad torácica es la tomografía computarizada, diversos estudios han establecido la utilidad de la ecografía torácica para diagnosticar distintas enfermedades pleuro-pulmonares como el derrame pleural y la consolidación pulmonar, entre otras. En este artículo mostramos los diferentes patrones ecográficos de la enfermedad pleuro-pulmonar, técnica cuya accesibilidad adquiere gran importancia en pacientes críticos (UCI, reanimación), porque evita trasladar al enfermo, no es invasiva y es fácilmente repetible. Por otro lado, permite visualizar directamente la enfermedad pleuro-pulmonar, hecho necesario para los procedimientos intervencionistas torácicos (AU)


Although the initial diagnosis and follow-up of pleuropulmonary disease are normally done with plain chest films and the gold standard for chest disease is computed tomography, diverse studies have established the usefulness of chest ultrasonography in the diagnosis of different pleuropulmonary diseases like pleural effusion and lung consolidation, among others. In this article, we show the different ultrasonographic patterns for pleuropulmonary disease. The availability of ultrasonography in different areas (ICU, recovery areas) makes this technique especially important for critical patients because it obviates the need to transfer the patient. Moreover, ultrasonography is noninvasive and easy to repeat. On the other hand, it enables the direct visualization of pleuropulmonary disease that is necessary for interventional procedures (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Pneumopatias , Derrame Pleural , Tórax/patologia , Tórax , Pneumotórax , Hidropneumotórax , Radiografia Torácica/instrumentação , Radiografia Torácica/métodos , Radiografia Torácica , Pleura/patologia , Pleura , Parede Torácica/patologia , Parede Torácica
20.
Radiologia ; 56(1): 52-60, 2014.
Artigo em Espanhol | MEDLINE | ID: mdl-22819690

RESUMO

Although the initial diagnosis and follow-up of pleuropulmonary disease are normally done with plain chest films and the gold standard for chest disease is computed tomography, diverse studies have established the usefulness of chest ultrasonography in the diagnosis of different pleuropulmonary diseases like pleural effusion and lung consolidation, among others. In this article, we show the different ultrasonographic patterns for pleuropulmonary disease. The availability of ultrasonography in different areas (ICU, recovery areas) makes this technique especially important for critical patients because it obviates the need to transfer the patient. Moreover, ultrasonography is noninvasive and easy to repeat. On the other hand, it enables the direct visualization of pleuropulmonary disease that is necessary for interventional procedures.


Assuntos
Pneumopatias/diagnóstico por imagem , Doenças Pleurais/diagnóstico por imagem , Humanos , Ultrassonografia
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