1.
J Nanosci Nanotechnol
; 17(4): 2861-864, 2017 04.
Artigo
em Inglês
| MEDLINE
| ID: mdl-29668218
RESUMO
The presented measurement and data analysis procedure reduces the read-out time for the volume-amplified magnetic nanobead detection assay from ~30 min to only 2 min, providing fast, sensitive detection of DNA molecules. The molecular detection and amplification protocol was verified using samples containing rolling circle-amplified DNA products formed from synthetic Vibrio cholerae target DNA, with a limit of detection of 5 pM. The developed read-out method could be used to rapidly identify pathogens in a variety of applications including target screening in hospitals with limited resources, in out-patient settings and in the field.
Assuntos
DNA Bacteriano/análise , Nanopartículas de Magnetita/química , Tipagem Molecular/métodos , Técnicas de Amplificação de Ácido Nucleico/métodos , DNA Bacteriano/química , Limite de Detecção , Vibrio cholerae/genética , Vibrio cholerae/isolamento & purificação
2.
Small
; 8(14): 2174-7, 2012 Jul 23.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22514097