RESUMO
No disponible
Assuntos
Humanos , Enterococcus faecium/patogenicidade , Infecções por Bactérias Gram-Positivas/complicações , Peritonite/microbiologia , Transplante de Fígado , Resistência a Vancomicina , Resistência Microbiana a Medicamentos , Enterococcus faecium , Hospedeiro ImunocomprometidoAssuntos
Infecção Hospitalar/tratamento farmacológico , Farmacorresistência Bacteriana Múltipla , Enterococcus faecium/efeitos dos fármacos , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Linezolida/farmacologia , Transplante de Fígado , Peritonite/tratamento farmacológico , Complicações Pós-Operatórias/tratamento farmacológico , Vancomicina/farmacologia , Atresia Biliar/cirurgia , Pré-Escolar , Coinfecção , Infecção Hospitalar/microbiologia , Farmacorresistência Bacteriana Múltipla/genética , Enterococcus faecium/genética , Infecções por Bactérias Gram-Positivas/microbiologia , Humanos , Linezolida/uso terapêutico , Masculino , Peritonite/microbiologia , Portoenterostomia Hepática , Complicações Pós-Operatórias/microbiologia , Infecções por Pseudomonas/tratamento farmacológico , RNA Bacteriano/genética , RNA Ribossômico 23S/genética , Vancomicina/uso terapêuticoRESUMO
We present a case of bacteraemia due to meticillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene. The susceptibility to meticillin of Staphylococcus aureus was investigated directly from one blood culture bottle using GenomEra MRSA/SA (Abacus Diagnostica Oy) test. This test identified S. aureus but the presence of the mecA gene result was negative, and the isolate was reported as meticillin-sensitive Staphylococcus aureus (MSSA). Susceptibility studies were done using VITEK 2 AST-P588 susceptibility cards (bioMérieux). The strain was identified as MRSA by the VITEK 2 system, although oxacillin MIC was low (0.5 µg ml(-1)). In view of these results, the isolate was tested for the presence of the mecC gene by a specific PCR and was verified as MRSA carrying mecC. The emergence of this new mecA homologue could have important consequences for the detection of MRSA when routine PCR methods are used as an identification method or provisional detection of MRSA, as in the case reported in this article, because S. aureus carrying the mecC gene will be wrongly diagnosed as meticillin susceptible. Negative results must be interpreted with caution and should be followed by conventional culture, and antimicrobial susceptibility testing or detection of mecC gene by a specific PCR.
Assuntos
Bacteriemia/diagnóstico , Carcinoma/microbiologia , DNA Bacteriano/genética , Staphylococcus aureus Resistente à Meticilina/genética , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Infecções Estafilocócicas/diagnóstico , Neoplasias da Bexiga Urinária/microbiologia , Idoso , Bacteriemia/microbiologia , Humanos , MasculinoRESUMO
We have developed an MLST-based scheme for typing Escherichia coli isolates using pyrosequencing of single nucleotide polymorphic positions (SNP). The SNP sequences are converted into allelic patterns and analyzed using the same approach used for MLST analyses. We have tested the method in two unselected collections of clinical isolates of E. coli obtained from blood and urine cultures. The two collections had a similar structure, 25% of the profiles (representing 68% of the isolates) were common to both, and 62% of the profiles (nearly 20% of the isolates) were unique. The four major profiles accounted for 44% of the isolates, and among these the most frequent one was related to the pandemic ST131 clone. The method is easy to implement and might be useful for typing large microbial collections.
