Moving Toward Standardized Toxicity Testing Procedures with Particulates by Dietary Exposure of Gammarids.

Ecotoxicological effect assessment of particulate materials and sparingly soluble substances is an emerging field. Current standard toxicity tests of aquatic organisms are based on soluble substances which are added to the aqueous phase. Although soluble substances distribute homogeneously, particles can form aggregates, resulting in inhomogeneous distribution and unpredictable exposure. Therefore, test scenarios need to be adapted to overcome these uncertainties. We present a dietary particle exposure tool for the toxicity testing of sparingly soluble substances or particles in combination with a standardizable food source for gammarids based on decomposition and consumption tablets (DECOTABs). Four food supplements in the DEOCOTAB formulation were compared to test their influence on the energy reserves of gammarids. Although feeding rate was constant for most supplements, mortality and energy reserves revealed clear differences. Tabs supplemented with algae-based phyll or animal protein-based trout food best met all of the requirements. Fluorescent plastic microparticles (10-65 µm) were homogenously distributed and stable in the DECOTABs. Constant feeding was observed, and the number of ingested microparticles by Gammarus roeseli was quantified in relation to the consumed food. The developed method provides a realistic and methodologically reliable uptake from the oral pathway and allows the quantification of inner exposition via feeding rate, providing a promising tool for standardized dietary exposure scenarios with particles. Environ Toxicol Chem 2021;40:1463-1476. © 2021 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Long-term effects of a catastrophic insecticide spill on stream invertebrates.

Accidental spills or illegal discharges of pesticides in aquatic ecosystems can lead to exposure levels that strongly exceed authorized pesticide concentrations, causing major impacts on aquatic ecosystems. Such short-term events often remain undetected in regular monitoring programs with infrequent sampling. In early spring 2015, we identified a catastrophic pesticide spill with the insecticide cypermethrin in the Holtemme River, Germany. Based on existing pre-event macroinvertebrate community data, we monitored the effects and recovery of the macroinvertebrate community for more than two years after the spill. Strong short-term effects were apparent for all taxa with the exception of Chironomidae and Tubificidae. Effects could also be observed on the community level as total abundance, taxa number and biomass strongly decreased. Total abundance and taxa number showed a fast recovery. Regarding long-term effects, the total biomass remained substantially below the pre-contamination level (76%) until the end of the study. Also the abundances of three taxa (Gammarus, Leuctra, Limnius Ad.) did not return to levels prior to the spill even after 26 months. This lack of the taxon-specific recovery was likely due to their long generation time and a low migration ability due to a restricted connectivity between the contaminated site and uncontaminated stream sections. These factors proved to be stronger predictors for the recovery than the pesticide tolerance. We revealed that the biological indicators SPEARpesticides and share of Ephemeroptera, Plecoptera and Trichoptera (EPT) are not suitable for the identification of such extreme events, when nearly all taxa are eradicated. Both indicators are functioning only when repeated stressors initiate long-term competitive replacement of sensitive by insensitive taxa. We conclude that pesticide spills can have significant long-term effects on stream macroinvertebrate communities. Regular ecological monitoring is imperative to identify such ecosystem impairments, combined with analytical chemistry methods to identify the potential sources of spills.