Cinnamaldehyde and eugenol protect against LPS-stimulated oxidative stress and inflammation in Raw 264.7 cells.

Macrophages are leukocytes that play a strategic role in immune response and can be associated with various diseases due to their effects on the inflammation process and oxidative events. The current study was evaluated the anti-inflammatory and antioxidant properties of cinnamaldehyde and eugenol, which are phyto-compounds with numerous bioactive properties, on lipopolysaccharide (LPS)-induced macrophage cells. For this purpose, Raw 264.7 cells were incubated with cinnamaldehyde or eugenol (15, 25, and 50 µM) then stimulated with LPS. After 24 hr, tumor necrosis factor-alpha (TNF-α), interleukin-1ß (IL-1ß), and IL-6 levels (as inflammatory mediators), and malondialdehyde (MDA) and nitric oxide (NOx) levels as well as superoxide dismutase (SOD) and catalase (CAT) activities (as oxidative status markers) were determined in cell cultures. Cinnamaldehyde and eugenol pre-treatments decreased TNF-α, IL-1ß, and IL-6 levels as compared to LPS group at all concentrations. Furthermore, these pre-treatments increased SOD activity while decreased MDA and NOx levels as well as CAT activity at different concentrations. Our results demonstrated that these phyto-compounds have potential for the treatment of various diseases as protective agents against chronic inflammation and oxidative stress. PRACTICAL APPLICATIONS: Chronic inflammation and oxidative stress are complications that play a detrimental role in the pathophysiology of many diseases. Alternative treatment methods have been investigated to prevent them. Cinnamaldehyde and eugenol are phyto-compounds with high bioactivity that can be obtained from foods and spices. In this study, the protective effects of cinnamaldehyde and eugenol on lipopolysaccharide-induced oxidative stress and inflammation in macrophage cells were investigated. According to the obtained results, cinnamaldehyde and eugenol pre-treatments decreased inflammation and also reduced oxidative stress. Cinnamaldehyde and eugenol may be a better natural alternative protective agent for the chronic inflammation- and oxidative stress-related diseases.

Investigation of the effects of local glutathione and chitosan administration on incisional oral mucosal wound healing in rabbits.

The aim of the present study was to investigate the effects of local glutathione (GSH) and chitosan applications on the oxidant events and histological changes that occur, during healing processes in rabbits with incisional intraoral mucosal wounds. For this purpose, discs containing glutathione and chitosan (1:1) were prepared and their physicochemical characteristics were evaluated. New Zealand white rabbits were used in in vivo studies. A standard incision was applied to the oral mucosa of rabbits. The rabbits were divided into four groups, being: an untreated incisional group (n=6), a group treated with discs containing GSH+chitosan (n=6), a group treated with discs containing solely chitosan (n=5) and a group treated with discs containing solely GSH (n=5). The levels of malondialdehyde (MDA), glutathione and nitric oxide (NOx) in the oral wound tissues were measured on the fifth day after the injury. Histological changes in the wound tissues were also investigated. The tissue MDA levels in the group treated with the disc containing GSH+chitosan were found to be lower than those in the other groups. There were no statistically significant differences in terms of tissue GSH and NOx levels between the group treated with the disc comprising GSH+chitosan and the control group that had untreated incision wounds. According to the histological findings, wound healing in the group treated with the disc containing solely chitosan was found to be better than in the other groups. The results of the experiments showed that the local application to the intraoral incision wounds of chitosan+GSH, and chitosan alone, can be effective in the wound healing processes of soft tissues and dental implants.