Does Ambroxol alleviate kidney ischemia-reperfusion injury in rats?

Objectives: Ischemia-reperfusion injury is a life-threatening clinical problem that can occur after transplantation or a number of clinical procedures. The purpose of the study was to investigate the effects of Ambroxol on kidney damage caused by experimentally induced ischemia-reperfusion injury in rats. Materials and Methods: Wistar albino rats were divided into 3 groups: Control (CTR, n=6), Kidney ischemia-reperfusion (K-IR, n=6), And kidney ischemia reperfusion+Ambroxol (K-IR+AMB, n=6). In K-IR+AMB group, Ambroxol (30 mg/kg) was administered orally 30 min before the ischemia period. K-IR and K-IR+AMB groups underwent 45 min of kidney ischemia followed by a 6-hour reperfusion period. At the end of the reperfusion period, blood and kidney tissue samples were collected after euthanasia. From the blood samples, BUN and creatinine levels were determined to assess kidney function, and TNF-α and IL-1ß concentrations were evaluated to determine inflammatory response. Results: While serum BUN, creatinine activities, and TNF-α and IL-1ß concentrations were higher in both IR groups compared with the CTR group, these values were found to be lower in the K-IR+AMB group compared with the K-IR group. Histopathological examination revealed that interstitial edema and desquamation of tubular cells in the K-IR group were more severe than in the K-IR+AMB group. Conclusion: Ambroxol treatment alleviated the production of pro-inflammatory cytokines and the harmful cellular effects in the tubular cells.

Could Ambroxol reduce cytokines in hepatic ischemia-reperfusion injury in rats?

OBJECTIVES: The aim of the study is to examine the effect of Ambroxol on TNF-α and IL-1ß released after liver ischemia-reperfusion injury. BACKGROUND: Many drugs are being tried to reduce ischemia-reperfusion injury, which is life threating problem after many liver surgeries. In this study, it was investigated whether Ambroxol reduces the release of pro-inflammatory cytokines released after liver ischemia-reperfusion injury. METHODS: Twenty-four Wistar albino rats were divided into 3 groups as Control (CTR; n=8), hepatic ischemia reperfusion (H-IR; n=8) and hepatic ischemia reperfusion+Ambroxol (H-IR+AMB; n=8). In H-IR+AMB group, Ambroxol (30 mg/kg) was administered orally 30 minutes before ischemia period. In H-IR and H-IR+AMB groups underwent 45 minutes of hepatic ischemia followed by a 60-minute reperfusion period. After reperfusion period, tissue and blood samples were collected from euthanised animals. ALT, AST, ALP, LDH, TNF-α, IL-1ß concentrations and liver tissues were evaluated. RESULTS: Serum ALT, ALP, AST, LDH, TNF-α and IL-1ß values were lower in the H-IR+AMB group compared to the H-IR group. In the histopathological examination, hepatocyte degeneration and congestion in the H-IR group were higher than in the H-IR+AMB group. CONCLUSION: It was determined that Ambroxol treatment suppressed the production of pro-inflammatory cytokines TNF-α and IL-1ß in rats undergoing hepatic ischemia reperfusion (Tab. 1, Fig. 2, Ref. 28).

An unusual case of traumatic dorsolateral sacral hernia with retroflexion of the urinary bladder in a cat.

Background: Perineal hernias are rarely seen in cats and can be caused by congenital or trauma. The urinary bladder is the most herniated organ and was being observed in prepubic, ventral perineal, umbilical, or caudoventral abdominal regions. Case Description: A 1-year-old female domestic short-haired cat was presented to the Animal Hospital of the Near East University Faculty of Veterinary Medicine with complaints of urinary retention as well as the inability to defecate. The patient owner reported that the cat had been struck by a car around 4 months previously, which resulted in a pelvic fracture. On clinical examination, a fluctuating mass was detected in the dorso-lateral region of the pelvis. After radiographic and ultrasonographic examination, the mass was diagnosed as the urinary bladder. A cystopexy operation was performed on the urinary bladder, which herniated through a muscular tear to the right dorsolateral pelvic region. Conclusion: Unusual case report of a sacral hernia resulting from a traumatic rupture of the sacral muscles together with urinary bladder retroflexion was reported in a cat.

Comparison of the analgesic effects of morphine and tramadol after tumor surgery in dogs.

BACKGROUND: Pain thresholds may differ between animal species. It may even vary among individuals of the same species. Therefore, assessing pain in animals is challenging. AIMS: The objective of the present study was to compare the analgesic effects of tramadol with morphine. METHODS: The study was carried out on randomly selected 20 dogs with tumors in different breeds and gender. After induction of anesthesia with propofol, dogs were intubated, and anesthesia was maintained with sevoflurane. Intravenous fentanyl citrate was used for intraoperative analgesia after stabilization of the anesthesia. When the tumors were surgically removed and the operation was completed, dogs were divided into two groups to give the postoperative analgesic agent. The first 10 dogs to be investigated were identified as the morphine group and the second 10 dogs as the tramadol group. Postoperative pain scores, heart rates, respiratory rates, and body temperatures were recorded at 0, 4, 8, 12, 16, 20, and 24 hours after the operation. RESULTS: Pain scores were lower in the morphine group than in the tramadol group during all postoperative processes. CONCLUSION: As a result, it was determined that tramadol has immediate analgesic effects than morphine; however, morphine provides better analgesia than tramadol.

Pleuroperitoneal true diaphragmatic hernia of the liver in a cat.

A 2-year old female domestic shorthair cat was referred with intermittent vomiting. Laboratory analyses revealed only an elevated alanine aminotransferase activity. Plain thoracic radiographs showed a right-sided transdiaphragm protrusion of a liver lobe into the thoracic cavity. On abdominal ultrasound the diaphragm and liver parenchyma also presented a bulge towards the thoracic cavity. By cranial median laparotomy herniation of the quadrate liver lobe through the right aspect of the diaphragm into the thoracic cavity was confirmed. The protruding quadrate liver lobe was covered by intact parietal peritoneum. Following relocation of the liver lobe the defect was closed. Postoperatively the intermittent vomiting stopped. The purpose of this case report was to present plain radiography and abdominal ultrasonography as effective tools in the diagnosis of this rare and usually asymptomatic true herniation case along with its operative treatment.

The role of transcavitary ultrasonography in diagnosis and staging of nonmuscle-invasive bladder cancer: a prospective non-randomized clinical study.

To evaluate the efficacy of cystoscopy, computed tomography (CT), transcavitary ultrasound (TCUS) and cytology, separately and in combination, for the diagnosis and evaluation of superficial bladder cancer. Initial cystoscopy and wash-out cytology were performed for 1548 patients. Of these, 206 with proven bladder tumors were included in this prospective study. CT and TCUS were performed for patients with bladder tumors without knowledge of their cystoscopy results. The lesions were classified as low- (pTa) and high- (pT1) risk superficial tumors according to multiplicity and size. Patients were divided into three categories according to their cystoscopically evaluated tumor size: ≤1 cm (88 patients, 42.7%), 1-3 cm (51 patients, 24.8%) and ≥3 cm (67 patients, 32.5%). TCUS identified 46 (22.3%) high-risk patients with/without invasion and 160 (77.7%) low-risk patients with no invasion. Overall, the sensitivity, specificity, positive predictive value and negative predictive value of TCUS for tumor detection were 77.4%, 60%, 94.7% and 22.2%, respectively. Cystoscopy remains the most widely used technique for the diagnosis of bladder cancer. The combined use of CT, TCUS and cytology detected 72% of cystoscopically proven tumors. Among the three, TCUS findings exhibited the strongest correlation with cystoscopy findings.