RESUMO
Irisin is mainly secreted by heart and skeletal muscle cells. It is an exercise-induced protein that converts white adipose tissue to brown. Increased irisin expression was lead to weight loss and improved glucose tolerance. We investigated irisin immunoreactivity in various tissues of the dwarf hamsters (Phodopus roborovskii). Tissues were processed, embedded in paraffin, sectioned at 5 µm and stained immunohistochemically for irisin. In the retina, irisin was found almost all layers, except outer nuclear layer. Also, irisin immunoreactivity was observed in the skin, cornea, striated muscle, parotid gland, tongue, oesophagus, stomach and small intestine. The findings from this study support the notion that skeletal muscle is not the primary source of irisin.
Assuntos
Fibronectinas/imunologia , Fibronectinas/metabolismo , Imuno-Histoquímica/veterinária , Animais , Córnea/metabolismo , Cricetinae , Esôfago/metabolismo , Mucosa Gástrica/metabolismo , Intestino Delgado/metabolismo , Músculo Esquelético/metabolismo , Glândula Parótida/metabolismo , Phodopus , Retina/metabolismo , Pele/metabolismo , Língua/metabolismoRESUMO
Irisin was first identified in skeletal muscle cells. It is an exercise protein that is secreted into the circulation; it causes conversion of white adipose tissue to brown adipose tissue. We investigated irisin immunoreactivity in mole rat (Spalax leucodon) tissues. We examined cerebellum, pituitary, heart, liver, pancreas, spleen, uterus, kidney and striated muscle in female adult mole rats. Tissues were processed, embedded in paraffin, sectioned at 5 µm and stained immunohistochemically for irisin. Irisin immunostaining was detected in the cytoplasm of stained cells; the cytoplasm of Purkinje cells was unstained. We found that irisin may be synthesized in many tissues. The function of locally synthesized irisin currently is unknown.
Assuntos
Estruturas Animais/metabolismo , Fibronectinas/metabolismo , Imuno-Histoquímica , Ratos-Toupeira , Animais , Feminino , Fibronectinas/análise , Músculo Esquelético/metabolismo , Células de Purkinje/metabolismo , Coloração e Rotulagem , Distribuição TecidualRESUMO
Androgens exert their effects through androgen receptors (AR) in tissues. We investigated the distribution of AR in female mole rat tissues. Tissues were excised, fixed with 10% formalin and embedded in paraffin. Sections were stained after microwave antigen retrieval for immunohistochemistry. Immunostaining of AR immunostaining was detected in the nucleus or cytoplasm of the cells in the cerebral cortex, cerebellum, anterior pituitary, lung, liver, uterus and skin. Granulosa and some thecal cells in the ovary, cardiac muscle cells and adipose cells exhibited a nuclear reaction for AR. In the kidney, labeling of AR was restricted to the cytoplasm of tubule cells. We found that AR could be detected using immunohistochemistry in the nucleus or cytoplasm or both in the presence of androgens.
Assuntos
Núcleo Celular/metabolismo , Imuno-Histoquímica , Ovário/metabolismo , Receptores Androgênicos/metabolismo , Animais , Feminino , Imuno-Histoquímica/métodos , Fígado/metabolismo , Masculino , Ratos-Toupeira , Útero/metabolismoRESUMO
Irisin was first identified in muscle cells. We detected irisin immunoreactivity in various organs of the crested porcupine (Hystrix cristata). In the epidermis, irisin immunoreactivity was localized mainly in stratum basale, stratum spinosum and stratum granulosum layers; immunoreactivity was not observed in the stratum corneum. In the dermis, irisin was found in the external and internal root sheath, cortex and medulla of hair follicles, and in sebaceous glands. Irisin immunoreactivity was found in the neural retina and skeletal muscle fibers associated with the eye. The pineal and thyroid glands also exhibited irisin immunoreactivity.