RESUMO
A new modification was designed to determine the activity of N-acetyl-beta-D-hexosaminidase (NAH) in biological fluids. The properties of enzyme molecules to adsorb themselves on chitin at pH 4.5 were made use of within the case study. The mean NAH activity, in urine of virtually healthy children and adults, was shown to be equal, within a span time of 1 h, to 12.8 and 22.3 mumole of formed 4-nitrophenol/per 1 mmole of creatinine. The above mean NAH parameter was, in the amniotic fluid of pregnant women, 696 nmole of 4-nitrophenol/per 1 ml in 1 h. The NAH activity was studied in renal pathologies and in delayed intrauterine development of the fetus.
Assuntos
Nefropatias/diagnóstico , beta-N-Acetil-Hexosaminidases/urina , Adolescente , Adulto , Biomarcadores/urina , Criança , Pré-Escolar , Feminino , Glomerulonefrite/diagnóstico , Glomerulonefrite/urina , Humanos , Nefropatias/urina , Masculino , Pessoa de Meia-Idade , GravidezRESUMO
A new method was suggested that provides for a simultaneous determination of protein- and oligomer-bound fucose in blood serum. The level of such compounds in blood serum was shown to be increasing with age and in certain diseases.
Assuntos
Análise Química do Sangue/métodos , Proteínas Sanguíneas/análise , Carboidratos/sangue , Fucose/sangue , Adulto , Criança , Pré-Escolar , Feminino , Glicolipídeos/sangue , Glicopeptídeos/sangue , Humanos , Masculino , Pessoa de Meia-Idade , Oligossacarídeos/sangueRESUMO
A method for lysozyme measurement in the urine is developed, based on adsorption of lysozyme molecules on the surface of chitin. The urine of healthy children, men, and women contains 1.76, 1.51, and 1.69 mg/liter lysozyme, respectively. Urinary lysozyme values in some diseases are presented.
