A relatively small 5' regulatory region of esterase S gene of Drosophila virilis determines the specific expression as revealed in transgenic experiments.

Expression of the esterase S gene of Drosophila virilis was studied in transgenic experiments. Truncated genomic copy of this gene including 400 bp of 5' regulatory region was integrated into the genome of Drosophila melanogaster. The products of the transferred gene were detected. It was found that strict temporal and tissue specificity of the esterase S gene expression is conserved in transformed flies. The results suggest that this specificity is evidently determined by the regulatory region of the esterase S gene and controlled by cis mechanism.

[Induction of unstable mutations in Drosophila melanogaster by microinjection of oncogenic virus DNA into polar embryonic plasm. Malignant effect of oncoviral DNA]. / Induktsiia nestabil'nykh mutatsii u Drosophila melanogaster mikroin''ektsei DNK onkogennykh virusov v poliarnuiu plazmu émbrionov. Maligniziruiushchii éffekt onkovirusnykh DNK.

We have demonstrated that the ability to induce benign neoplasms We have dominant mode of inheritance in Drosophila melanogaster is the specific feature of oncoviral DNAs. It is supposed that development of this type of neoplasms in Drosophila is connected with the changes in expression of protooncogenes in mutant genome: firstly, the genetic factors directing the development of neoplasms and Drosophila protooncogenes which shared the homology with v-src are localised in the same regions; secondly, there are structural rearrangements in c-src/fps (29A) protooncogene in mutant stocks which display the ability for neoplastic growth.

[Induction of unstable mutations in Drosophila melanogaster by microinjections of oncogenic virus DNA into the embryo polar plasma. Prolonged genetic instability of mutations at the lobe locus]. / Induktsiia nestabil'nykh mutatsii u Drosophila melanogaster mikroin"ektsiei DNK onkogennykh virusov v poliarnuiu plazmu émbrionov. Prodlennaia genet'icheskaia nestabil'nost' mutatsitii v lokuse lobe.

Mutations Lobe induced by the microinjections of RSV cDNA into Drosophila melanogaster early embryos are characterized by permanent genetic instability; the level of this instability is being changed in time. Based on the results of genetic analyses of Lobe mutations and molecular analysis of white and ADH mutations induced at high frequency in this system of gene instability, we supposed that unstable mutations which arose under the influence of retroviral cDNA are of the insertional nature.

INDUCTION OF UNSTABLE MUTATIONS IN DROSOPHILA MELANOGASTER BY THE MICROINJECTION OF ONCOGENIC VIRAL DNA INTO THE POLAR PLASM OF EMBRYOS: PROLONGED GENETIC INSTABILITY OF MUTATIONS AT THE LOBE LOCUS.

MUTATIONS IN THE LOBE LOCUS WERE PRODUCED BY MICROINJECTION OF RSV CDNA INTO EARLY EMBRYOS OF D. MELANOGASTER, AND SHOWED PROLONGED GENETIC INSTABILITY, THE EXTENT OF WHICH CHANGED SIGNIFICANTLY WITH TIME. GENETIC ANALYSIS OF LOBE-RSV MUTATIONS AND A MOLECULAR-BIOLOGICAL ANALYSIS OF STRUCTURAL REARRANGEMENTS IN THE W AND ADH LOCI, WHICH ARE FREQUENTLY MUTATED BY RETROVIRAL CDNA, SHOWED THAT THE MUTATIONS RESULTED FROM INSERTIONS ARISING IN THIS GENETIC INSTABILITY SYSTEM.