RESUMO
1. Common carp (Cyprinus carpio L.) liver Cu,Zn-superoxide dismutase (Cu,Zn-SOD) was purified and characterized. 2. Its molecular weight, isoelectric point, electrophoretic mobility, amino acid pattern and some other characteristics were determined.
Assuntos
Carpas/metabolismo , Cyprinidae/metabolismo , Fígado/enzimologia , Superóxido Dismutase/metabolismo , Aminoácidos/análise , Animais , Feminino , Ponto Isoelétrico , Isoenzimas/metabolismo , Masculino , Peso Molecular , Superóxido Dismutase/isolamento & purificaçãoRESUMO
The effects of paraquat on the superoxide dismutase, catalase, glutathionine peroxidase activities and lipid peroxidation at different times of paraquat exposure of Cyprinus carpio morph L. erythrocytes were studied. Typical characteristics were observed in the changes of the enzyme activities of the erythrocytes after exposure to paraquat. The haemoglobin concentration of common carp haemolysates was decreased by exposure to paraquat.
Assuntos
Carpas/sangue , Catalase/sangue , Cyprinidae/sangue , Eritrócitos/enzimologia , Glutationa Peroxidase/sangue , Peróxidos Lipídicos/sangue , Paraquat/farmacologia , Superóxido Dismutase/sangue , Animais , Eritrócitos/efeitos dos fármacos , CinéticaRESUMO
The activities of Cu,Zn-superoxide dismutase, Mn-superoxide dismutase, catalase and glutathione peroxidase were comparatively studied in organ homogenates from two herbivorous fishes, viz., the grass carp and the silver carp. 2. The protein contents and lipid peroxidation of organ homogenates were also compared. The comparative measurements primarily provide control values for subsequent toxicological examinations. 3. The highest total superoxide dismutase activities were found in the kidney, spleen and liver in the grass carp, and in the kidney and liver in the silver carp. 4. The antioxidant enzyme activities and other parameters of the organ homogenates appear to be independent of the feeding mode, but are rather characteristic of the fish variety.
Assuntos
Carpas/metabolismo , Cyprinidae/metabolismo , Peróxidos/metabolismo , Superóxido Dismutase/metabolismo , Animais , Catalase/metabolismo , Glutationa Peroxidase/metabolismo , Peróxidos Lipídicos/metabolismo , Especificidade da Espécie , Distribuição TecidualRESUMO
Comparative studies were made of the activities of Cu,Zn-superoxide dismutase, Mn-superoxide dismutase, catalase and glutathione peroxidase in organ homogenates from two herbivorous (grass carp and silver carp) and three omnivorous fish (barbel, crucian carp and common carp). The protein contents and lipid peroxidation of the organ homogenates were also compared. These comparative measurements provided control values for subsequent toxicological examinations. For the herbivorous fish, the highest total superoxide dismutase activity was observed in the kidney, followed in turn by the liver or spleen. In contrast, the highest total superoxide dismutase activity in the barbel was found in the roe, in the crucian carp in the liver, and in the common carp in the liver too. The quantitative distributions of the two peroxide metabolism enzymes glutathione peroxidase and catalase in the organ homogenates present very varied pictures. The results of the lipid peroxidation are similarly difficult to fit into one framework, even for fish following the same diet. It appears that the enzyme activities mentioned in points 4 and 5, together with the lipid peroxidation, depend not on the species, but on the variety.