RESUMO
Hydrogen peroxide has been found to have a distorting effect on the quality of determination of the serological markers of hepatitis B and C, transglutaminase antibodies: an increase in the percent of false higher (anti-HBsAg, HBeAg, anti-HCV) and false lower (anti-HBeAg) values, and on the results of PCR-based diagnosis (PCR inhibition that was more pronounced especially in low viremia). A possibility of interference of measurement results in the blood metabolite pool should be taken into account in the use of high-technology methods of laboratory analysis. In particular, there may be changes in the detection of immunological and molecular biological methods in hyperpyruvatemia and hyperoxaloacetatemia, with elevated peroxide concentrations during pathological processes.
Assuntos
Hepatite B Crônica/sangue , Hepatite C Crônica/sangue , Biomarcadores/sangue , Técnicas Eletroquímicas , Etanol/sangue , Reações Falso-Negativas , Reações Falso-Positivas , Anticorpos Anti-Hepatite B/sangue , Antígenos da Hepatite B/sangue , Hepatite B Crônica/virologia , Anticorpos Anti-Hepatite C/sangue , Hepatite C Crônica/virologia , Humanos , Peróxido de Hidrogênio/sangue , Técnicas Imunoenzimáticas , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Medições Luminescentes , Oxaloacetatos/sangue , Reação em Cadeia da Polimerase , Ácido Pirúvico/sangue , Transglutaminases/imunologiaRESUMO
The performed studies provided new evidence for biological variability in clinically important analytes, estimated a possible association of the metabolic features and the conservative genetic attribute--the ABO blood group. Certain features in the levels of protein, carbohydrate, lipid, and mineral metabolic parameters were revealed in relation to various blood groups.
