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3.
Biochem J ; 195(2): 471-80, 1981 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-7316962

RESUMO

Preformed immune aggregates, containing antigen and either IgG (immunoglobulin G) or F(ab')2 rabbit antibody, were incubated with normal human serum under conditions allowing activation of only the alternative pathway of complement. Both the IgG and F(ab')2 immune aggregates bound C3b, the activated form of the complement component C3, in a similar manner, 2-3% of the C3 available in the serum being bound to the aggregates as C3b, and the rest remaining in the fluid phase as inactive C3b or uncleaved C3. It was found that the C3b was probably covalently bound to the IgG in the aggregates, since C3b-IgG complexes could be demonstrated on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, after repeated washing with buffers containing high salt or boiling under denaturing conditions. Incubation of the C3b-antibody-antigen aggregates in buffers known to destroy ester linkages had little effect on the C3b-IgG complexes, which suggested that C3b and IgG might be linked by an amide bond. Two main types of C3b-IgG complexes were found that had apparent mol.wts. of 360000 and 580000, corresponding to either one to two C3b molecules respectively bound to one molecule of antibody. On reduction of the C3b-IgG complexes it was found that the beta-chain, but not the alpha'-chain, of C3b was released along with all the light chain of IgG but only about half or less of the heavy chain of IgG. These results indicate that, during activation of the alternative pathway of complement by immune aggregates containing IgG antibody, the alpha'-chain of C3b may become covalently bound at one or two sites in the Fd portion of the heavy chain of IgG.


Assuntos
Complexo Antígeno-Anticorpo/metabolismo , Ativação do Complemento , Complemento C3b/metabolismo , Via Alternativa do Complemento , Autorradiografia , Sítios de Ligação , Proteínas Inativadoras do Complemento C3b/farmacologia , Eletroforese em Gel de Poliacrilamida , Humanos , Imunoglobulina G/metabolismo , Técnicas In Vitro , Peso Molecular , Ligação Proteica
4.
Immunology ; 42(1): 75-82, 1981 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-6780451

RESUMO

Immune aggregates formed from either rabbit IgG antibody or F(ab')2 antibody, and antigen, caused exactly the same extent of incubated with human serum under conditions when incubated with human serum under conditions allowing only alternative pathway activation. These observations confirm that the F(ab')2 region of the antibody molecule can cause alternative pathway activation and that this activation is not affected by the presence of the Fc region of the molecule when only alternative pathway activation is permitted. Under conditions allowing activation of both the classical and alternative pathways, increased alternative pathway activation was obtained with IgG antibody aggregates compared to that obtained with F(ab')2 antibody aggregates. On reduction and alkylation of principally he inter-heavy-chain disulphide bond in the IgG antibody, prior to aggregate formation, it was found that no activation of the alternative pathway by IgG aggregates took place under conditions allowing only alternative pathway activation. Treatment of the IgG antibody with reducing agent alone, or alkylating reagent alone, followed by dialysis and aggregate formation, yielded aggregates which caused alternative pathway activation values close to those obtained for untreated IgG aggregates. These results indicate that the integrity of the inter-heavy-chain disulphide bond of rabbit IgG antibody in immune aggregates is necessary to allow the F(ab')2 region of the IgG molecule to activate the alternative pathway of human complement.


Assuntos
Ativação do Complemento , Via Alternativa do Complemento , Fragmentos Fab das Imunoglobulinas/imunologia , Imunoglobulina G/imunologia , Animais , Complexo Antígeno-Anticorpo , Sítios de Ligação , Via Clássica do Complemento , Dissulfetos , Hemólise , Humanos , Cadeias Pesadas de Imunoglobulinas , Coelhos
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