Thyroid morphometry and intrathyroidal 131-I iodine transit kinetics in Kashmiri population.

Morphometric--kinetic parameters of the thyroid gland in the Kashmiri population, which is geographically, environmentally, socioculturally, anthropologically and ethnically different from that of the main country (Indian republic), have been analysed. Morphometrically, the gland in this population has been found to be significantly larger (p less than 0.001), while kinetically, the A max which is the ordinate-extrapolate of the best-fit RAIU(t) curve, has been found to be significantly lower (p less than 0.01). The majority of other structural and functional parameters were similar to those of the north Indian population.

Response of mice testis to gossypol acetic acid.

Male mice were administered gossypol (2.5, 5.0, 10.0 and 20.0 mg/kg body weight) by oral intubation, subcutaneous and intraperitoneal routes for 30 days. The drug treatment did not have any effect on body weight and organ weights. The motility of cauda epididymis and vas deferens spermatozoa was not affected by gossypol treatment nor there was any effect of gossypol on the histoarchitecture of the testis irrespective of the dose and route of administration. Maximum mortality was observed in mice when animals were given gossypol by intraperitoneal route.

Studies on the male antifertility agent gossypol acetic acid. VII. Effect of motility stimulated factors on the revival of human spermatozoal motility after gossypol treatment in vitro.

Human spermatozoa were incubated with gossypol acetic acid (100 micrograms/1 X 10(6) spermatozoa/ml) at 37 degrees C for 30 min. The drug treatment inhibited the spermatozoal motility significantly. Washing of the spermatozoa, after gossypol treatment, did not effect their motility. A partial revival in the motility of the spermatozoa was observed when gossypol treated spermatozoa were incubated, after washing, with motility stimulating factors, e.g. theophylline, dibutyryl-cAMP and Kallikrein.

PIP: This brief experiment asked the question whether the inhibition of sperm motility imposed by gossypol is reversible by washing away the polyphenol. Human spermatozoa were incubated in Bakers buffer, 1 million/ml at 37 degrees C. for 30 minutes in the presence of 100 mc gossypol. Gossypol was dissolved in ethanol and diluted to 0.01% with distilled water, then 0.01 ml was added to 1.0 ml spermatozoa. Controls contained 0.1 ml of 0.01% ethyl acetate. Gossypol reduced motility from a range of 66.1 to 79.4% to a range of 32.1 to 38.1% (p0.01). Washing the spermatozoa by centrifuging twice at 343 x g for 10 min. and reincubating in a buffer had no effect on motility. Addition of the sperm motility stimulating agents theophylline 10 mM, kallikrein 100 KIU or dibutyryl-caMP after washing away the gossypol restored motility to 55.1%, 58.7% and 50.0%, respectively (p0.01).