Impacts of salinity stress on crop plants: improving salt tolerance through genetic and molecular dissection.

Improper use of water resources in irrigation that contain a significant amount of salts, faulty agronomic practices such as improper fertilization, climate change etc. are gradually increasing soil salinity of arable lands across the globe. It is one of the major abiotic factors that inhibits overall plant growth through ionic imbalance, osmotic stress, oxidative stress, and reduced nutrient uptake. Plants have evolved with several adaptation strategies at morphological and molecular levels to withstand salinity stress. Among various approaches, harnessing the crop genetic variability across different genepools and developing salinity tolerant crop plants offer the most sustainable way of salt stress mitigation. Some important major genetic determinants controlling salinity tolerance have been uncovered using classical genetic approaches. However, its complex inheritance pattern makes breeding for salinity tolerance challenging. Subsequently, advances in sequence based breeding approaches and functional genomics have greatly assisted in underpinning novel genetic variants controlling salinity tolerance in plants at the whole genome level. This current review aims to shed light on physiological, biochemical, and molecular responses under salt stress, defense mechanisms of plants, underlying genetics of salt tolerance through bi-parental QTL mapping and Genome Wide Association Studies, and implication of Genomic Selection to breed salt tolerant lines.

Unveiling Nilaparvata lugens Stål Genes Defining Compatible and Incompatible Interactions with Rice through Transcriptome Analysis and Gene Silencing.

The brown planthopper (Nilaparvata lugens Stål, BPH) is a major pest of rice (Oryza sativa L.), causing severe crop loss. Multiple biotypes and emerging populations of BPH pose a bigger challenge for the infestations control. Although several studies have been conducted to understand the molecular mechanisms of rice-BPH interactions, there are few studies dedicated to the Indian sub-continent BPH biotype (biotype 4). Here, we analyzed the transcriptomic, physiological, and gene-silencing responses of the BPH biotype 4 during the compatible (fed on susceptible Taichung Native 1, TN1 rice) and incompatible (fed on resistant PTB33 rice) rice-BPH interactions. In the incompatible interaction, a significant reduction in the honeydew production and negative weight gain were observed in the BPH. Similarly, the trehalose and glucose contents were found to be significantly high and low, respectively, during the incompatible rice-BPH interaction. The comparative BPH transcriptome analysis identified 1875 differentially expressive genes (DEGs) between the compatible and incompatible interactions from which many were annotated to be involved in vital BPH physiological processes, including cuticle development, sugar metabolism, detoxification, molting, and xenobiotics metabolism. The RNA interference-mediated independent silencing of three selected genes, including NlCP1, NlCYP320a1, and NlTret1, revealed that these genes are important for BPH physiology and survival. Moreover, the results of this study provide valuable insights into the rice-BPH interactions involving the BPH biotype 4.