Assuntos
Radioimunoensaio/métodos , Doenças da Glândula Tireoide/diagnóstico , Hormônio Liberador de Tireotropina/sangue , Animais , Formação de Anticorpos , Reações Cruzadas , Estudos de Avaliação como Assunto , Feminino , Bócio Nodular/diagnóstico , Doença de Graves/diagnóstico , Humanos , Hipotireoidismo/diagnóstico , Soros Imunes , Radioisótopos do Iodo , Masculino , Coelhos , Doenças da Glândula Tireoide/sangueRESUMO
Emission computerized axial tomography is achievable in any nuclear medicine department from multiple gamma camera views. Data are collected by rotating the patient in front of the camera. A simple fast algorithm is implemented, known as the convolution technique: first the projection data are Fourier transformed and then an original filter designed for optimizing resolution and noise suppression is applied; finally the inverse transform of the latter operation is back-projected. This program, which can also take into account the attenuation for single photon events, was executed with good results on phantoms and patients. We think that it can be easily implemented for specific diagnostic problems.
Assuntos
Tomografia Computadorizada de Emissão , Encéfalo/diagnóstico por imagem , Filtração , Análise de Fourier , Raios gama , Humanos , Fígado/diagnóstico por imagem , Radiografia , Tomografia Computadorizada de Emissão/instrumentaçãoRESUMO
We describe the preliminary steps for a radio-immunoassay of Thyrotropin Releasing Hormone (TRH). Rabbit antiserum at dilution 1 : 10 000 is used with radioiodinated TRH (125I). We are able to assay from 5 to 1 000 pg unlabeled TRH with an intraassay reporducibility varying from 7 to 4 % and the lowest detectable amount in this system is 10 pg TRH. TRH mean and standard deviation in normal subjects are 136,9 and 25,3 pg/ml.