Thermodynamic characterization of a non-commercial emulsifying agent for asphalt emulsion.

The objective of the present work was to characterize a non-commercial emulsifying agent: dodecylpyridinium thiocyanate (C12PCNS). This study was carried out by conductimetry technique in the temperature range 25-50 °C. Assuming the system conforms to the pseudo-phase separation model, change of standard Gibbs free energy, ΔGm0, enthalpy, ΔHm0, and entropy of micellization, ΔSm0, were estimated. Values for critical micelle concentration (cmc) and the ionization degree, ß, were determined directly from the experimental data. The results show that the influence of the counter ion is very relevant, because the cmc for this compound is appreciably greater than the cmc of other pyridinium homologs of the same chain length. Finally, the compensation rule for this system has been found and the compensation temperature and the intercept ΔH*-it is an indication of the stability of the micelle-were estimated.

Multicenter laboratory evaluation of the MB/BacT Mycobacterium detection system and the BACTEC MGIT 960 system in comparison with the BACTEC 460TB system for susceptibility testing of Mycobacterium tuberculosis.

In this multicenter study, the reliability of two nonradiometric, fully automated systems, the MB/BacT and BACTEC MGIT 960 systems, for testing the susceptibilities of 82 Mycobacterium tuberculosis strains to isoniazid, rifampin, ethambutol, and streptomycin was evaluated in comparison with the radiometric BACTEC 460TB system. The arbitration of discrepant results was done by the reanalysis of the strain, the determination of the MIC, and the molecular characterization of some resistance determinants. The overall level of agreement with BACTEC 460TB results was 96% with the MB/BacT test and 97.2% with the BACTEC MGIT 960 system. With both methods, the level of agreement with BACTEC 460TB results was 96.3% for isoniazid, 98.8% for rifampin, and 98.8% for ethambutol. The level of agreement for streptomycin was 90.2% with MB/BacT and 97.5% with BACTEC MGIT 960. Overall, there were 11 very major errors and 2 major errors with the MB/BacT method and 5 very major errors and 2 major errors with the BACTEC MGIT 960 system. In general, the MB/BacT and BACTEC MGIT 960 systems showed good performance for susceptibility testing with first-line antituberculosis drugs.