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1.
J Med Chem ; 56(15): 6248-58, 2013 Aug 08.
Artigo em Inglês | MEDLINE | ID: mdl-23841482

RESUMO

Helicobacter pylori (Hp) infection affects one-half of the human population and produces a variety of diseases from peptic ulcer to cancer. Current eradication therapies achieve modest success rates (around 70%), resistance to the antibiotics of choice is on the rise, and vaccination has not proved to be successful yet. Using an essential Hp protein, flavodoxin, as target, we identified three low-molecular-weight flavodoxin inhibitors with bactericidal anti-Hp properties. To improve their therapeutic indexes, we have now identified and tested 123 related compounds. We have first tested similar compounds available. Then we have designed, synthesized, and tested novel variants for affinity to flavodoxin, MIC for Hp, cytotoxicity, and bactericidal effect. Some are novel bactericidal inhibitors with therapeutic indexes of 9, 38 and 12, significantly higher than those of their corresponding leads. Developing novel Hp-specific antibiotics will help fighting Hp resistance and may have the advantage of not generally perturbing the bacterial flora.


Assuntos
Antibacterianos/síntese química , Flavodoxina/antagonistas & inibidores , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori/efeitos dos fármacos , Antibacterianos/química , Antibacterianos/farmacologia , Benzopiranos/síntese química , Benzopiranos/química , Benzopiranos/farmacologia , Células HeLa , Humanos , Testes de Sensibilidade Microbiana , Modelos Moleculares , Oxidiazóis/síntese química , Oxidiazóis/química , Oxidiazóis/farmacologia , Ligação Proteica , Relação Estrutura-Atividade , Estirenos/síntese química , Estirenos/química , Estirenos/farmacologia
2.
Antimicrob Agents Chemother ; 57(3): 1231-7, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23263004

RESUMO

Helicobacter pylori is a globally important and genetically diverse gastric pathogen that infects most people in developing countries. Eradication efforts are complicated by antibiotic resistance, which varies in frequency geographically. There are very few data on resistance in African strains. Sixty-four Gambian H. pylori strains were tested for antibiotic susceptibility. The role of rdxA in metronidazole (Mtz) susceptibility was tested by DNA transformation and sequencing; RdxA protein variants were interpreted in terms of RdxA structure. Forty-four strains (69%) were resistant to at least 8 µg of Mtz/ml. All six strains from infants, but only 24% of strains from adults, were sensitive (P = 0.0031). Representative Mtz-resistant (Mtz(r)) strains were rendered Mtz susceptible (Mtz(s)) by transformation with a functional rdxA gene; conversely, Mtz(s) strains were rendered Mtz(r) by rdxA inactivation. Many mutations were found by Gambian H. pylori rdxA sequencing; mutations that probably inactivated rdxA in Mtz(r) strains were identified and explained using RdxA protein's structure. All of the strains were sensitive to clarithromycin and erythromycin. Amoxicillin and tetracycline resistance was rare. Sequence analysis indicated that most tetracycline resistance, when found, was not due to 16S rRNA gene mutations. These data suggest caution in the use of Mtz-based therapies in The Gambia. The increasing use of macrolides against respiratory infections in The Gambia calls for continued antibiotic susceptibility monitoring. The rich variety of rdxA mutations that we found will be useful in further structure-function studies of RdxA, the enzyme responsible for Mtz susceptibility in this important pathogen.


Assuntos
Antibacterianos/farmacologia , Proteínas de Bactérias/genética , Farmacorresistência Bacteriana/genética , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori/genética , Mutação , Nitrorredutases/genética , Adolescente , Adulto , Idoso , Amoxicilina/farmacologia , Criança , Pré-Escolar , Claritromicina/farmacologia , Análise Mutacional de DNA , Farmacorresistência Bacteriana/efeitos dos fármacos , Eritromicina/farmacologia , Feminino , Gâmbia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/efeitos dos fármacos , Helicobacter pylori/isolamento & purificação , Humanos , Lactente , Masculino , Metronidazol/farmacologia , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , RNA Ribossômico 16S/genética , Tetraciclina/farmacologia
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