RESUMO
The influence of magnitolaser radiation on the striated acetylcholinergic neurons was tested on the tissue culture derived from a newborn rat's brain. After 5 days of cultivation of brain cells, the authors carried out 10 everyday sessions using a magnitolaser "KMILTA" with infrared radiation. It was established on the bases of obtained results that AchE activity reliably increases in 1,3-fold compared with the control group. Thus magnitolaser radiation stimulates activation of regenerative processes in the striatal acetylcholinergic neurons.
Assuntos
Acetilcolina/metabolismo , Acetilcolinesterase/metabolismo , Raios Infravermelhos , Lasers , Magnetismo , Neurônios/efeitos da radiação , Animais , Animais Recém-Nascidos , Células Cultivadas , Corpo Estriado/citologia , Neurônios/enzimologia , Neurônios/metabolismo , RatosRESUMO
Active anti-stroke fraction of Cerebral preparation (extract of water-soluble molecules from brain tissue of animals with hemorrhagic stroke) decreased caspase-3 expression and improved survival of experimental animals in the acute period after hemorrhagic stroke.
Assuntos
Precursor de Proteína beta-Amiloide/antagonistas & inibidores , Inibidores de Caspase , Hemorragia Cerebral/tratamento farmacológico , Acidente Vascular Cerebral/tratamento farmacológico , Extratos de Tecidos/uso terapêutico , Doença Aguda , Precursor de Proteína beta-Amiloide/genética , Precursor de Proteína beta-Amiloide/metabolismo , Animais , Caspase 3 , Caspases/genética , Caspases/metabolismo , Hemorragia Cerebral/enzimologia , Hemorragia Cerebral/metabolismo , Masculino , RNA Mensageiro/análise , RNA Mensageiro/metabolismo , Ratos , Acidente Vascular Cerebral/enzimologia , Acidente Vascular Cerebral/metabolismo , Extratos de Tecidos/farmacologiaRESUMO
A homogeneous low-molecular-weight fraction (MW, 350-500 Da) separated from a ready-to-use commercial form of the new neurotropic drug cerebral (for amino acid composition see Arkh. Psikh. 3(18), 138-143 (1998)) increases the survival of experimental rats upon experimental hemorrhagic stroke (HS). The distribution and accumulation of tritium-labeled preparation was studied in various tissues (tractus olfactorius, bulbus olfactorius, damaged neocortex region, hippocampus, hypothalamus, midbrain, pons, medulla oblongata, cerebrum, liver) and in the blood of intact, shame-operated (SO), and HS rats 20 and 120 min after intranasal administration. A significant amount of the labeled drug is accumulated for 20 min in all structures of the brain, liver, and blood. After 120 min, the level of accumulated drug proportionally increases. The amount of labeled preparation accumulated in SO and HS rats is greater than that in intact rats. The character of cerebral accumulation in tractus olfactorius and bulbus olfactorius upon intranasal administration is indicative of the effective drug transport to CNS via these pathways. Therefore, the intranasal administration can offer a promising means of clinical treatment of HS patients.
