RESUMO
Considering that Ankistrodesmus falcatus is very sensitive to different pollutants, in this work the effect of lead, carbaryl and a mixture of both pollutants on protein, chlorophyll and phenols concentration in this microalga have been studied. At different lots of Ankistrodesmus falcatus in the middle of the log growth phase, different concentrations of lead, carbaryl and a mixture of both pollutants were added, during 24, 48 and 72 h. Chlorophyll, proteins and phenols concentration was measured. The results show that the mixture of lead-carbaryl produces a major toxic effect than the xenobiotics by themselves, so it has been suggested that to establish permissible limits it is necessary to consider the synergism presented in simultaneous exposure to both xenobiotics. It is suggested to use phenols determination as a primary indicator of environmental impact in an aquatic ecosystem.