1.
Rev Clin Esp
; 164(6): 395-7, 1982 Mar 31.
Artigo
em Espanhol
| MEDLINE
| ID: mdl-7111805
Assuntos
Lipase/metabolismo , Microssomos Hepáticos/enzimologia , Animais , Cinética , Ratos , Ratos Endogâmicos
2.
Enzyme
; 27(3): 215-9, 1982.
Artigo
em Inglês
| MEDLINE
| ID: mdl-7075586
RESUMO
An assay is described for the determination of lipase activity, using as substrate an emulsion of radioactive triolein with cholic acid as emulsifier. Lipase activity is given by determining radioactive triolein disappearance after incubation with the artificial emulsion. The catalytic activity of this enzyme has been studied with respect to time, protein concentration and substrate concentration. Under the experimental conditions, Michaelis constant value was 14.54 mmol/l and maximal hydrolysis rate 0.31 mumol h-1 mg-1.
Assuntos
Lipase/metabolismo , Fígado/enzimologia , Animais , Cinética , Ratos , Ratos Endogâmicos , Solubilidade , Fatores de Tempo , Trioleína/metabolismo
3.
Rev Clin Esp
; 160(5): 287-91, 1981 Mar 15.
Artigo
em Espanhol
| MEDLINE
| ID: mdl-7255787