Effects of social and environmental contexts on multi-male mating and mixed paternity in socially monogamous female prairie voles.

With whom and how often to mate are fundamental questions that impact individual reproductive success and the mating system. Relatively few studies have investigated female mating tactics compared with males. Here, we asked how differential access to mates influences the occurrence of mixed paternity and overall reproductive success in socially monogamous female prairie voles (Microtus ochrogaster). We created male- and female-biased sex ratios of prairie voles living in semi-natural outdoor enclosures. We ran paternity analyses to determine the identity and number of mating partners females had and the number of offspring produced. We found that 57.1% of females had litters fathered by two or more males when males outnumbered females, and 87.5% of females had litters with more than one father when females outnumbered males. However, the percentage of mixed paternity and the total number of embryos were not statistically different between social contexts. We determined that female fecundity (i.e. number of embryos) correlated with the number of male fathers in each litter across social contexts. Although our study did not support the hypothesis that social context directly influences female mating decisions, it did suggest that female multi-male mating might lead to increased fertilization success under semi-natural conditions.

Differential distribution of steroid hormone signaling networks in the human choroid-retinal pigment epithelial complex.

BACKGROUND: The retinal pigment epithelium (RPE), a layer of pigmented cells that lies between the neurosensory retina and the underlying choroid, plays a critical role in maintaining the functional integrity of photoreceptor cells and in mediating communication between the neurosensory retina and choroid. Prior studies have demonstrated neurotrophic effects of select steroids that mitigate the development and progression of retinal degenerative diseases via an array of distinct mechanisms of action. METHODS: Here, we identified major steroid hormone signaling pathways and their key functional protein constituents controlling steroid hormone signaling, which are potentially involved in the mitigation or propagation of retinal degenerative processes, from human proteome datasets with respect to their relative abundances in the retinal periphery, macula, and fovea. RESULTS: Androgen, glucocorticoid, and progesterone signaling networks were identified and displayed differential distribution patterns within these three anatomically distinct regions of the choroid-retinal pigment epithelial complex. Classical and non-classical estrogen and mineralocorticoid receptors were not identified. CONCLUSION: Identified differential distribution patterns suggest both selective susceptibility to chronic neurodegenerative disease processes, as well as potential substrates for drug target discovery and novel drug development focused on steroid signaling pathways in the choroid-RPE.