Can soil phosphorus availability in tropical forest systems be increased by nitrogen-fixing leguminous trees?

Understanding the role of N-fixing leguminous trees for phosphorus (P) cycling in highly weathered tropical soils is relevant for the conservation of natural forests as well as the sustainable management of agroforests and forest plantations with low P input in the Brazilian Atlantic Forest region. We hypothesized that N-fixing leguminous trees can increase the availability of soil P by exploiting different P sources without causing a depletion of soil organic P due to efficient biogeochemical cycling, but empirical evidence remains scarce. For this purpose, 31P nuclear magnetic resonance spectroscopy (31P NMR) was used for quantifying soil P forms and the Hedley sequential extraction to determine soil P fractions. The studied sites were forestry systems with leguminous trees: mixed forest plantations with different proportions of fast-growing N-fixing leguminous trees; pure plantations, and agroforestry systems with leguminous trees. The results show that all N-fixing leguminous trees and N mineral fertilization positively affected the concentrations of available soil P in relation to the control treatments. There were increases of all P fractions through cycling in all forest sites. 31P NMR spectra clearly identified and quantified that a large amount of phosphomonoesters followed by phosphodiesters in the form of DNA, as well as high reserves of Pi species (ortho-P and pyrophosphate) in the first eleven years of growth at pure plantations, mixed plantations or agroforests. The relations between both ortho-P and DNA with the resin-Pi, NaHCO3-Pi and NaOH-Pi fractions suggest that both analysis methods provide complementary information about the soil P transformations. Thus, the paper highlights the importance of the use of different N-fixing leguminous tree species under different environmental conditions, production systems and management practices for recovering heavily degraded areas, which may be a suitable strategy through efficient management of P in highly weathered tropical soils in the Brazilian Atlantic Forest biome.

The relationships between microbiological attributes and soil and litter quality in pure and mixed stands of native tree species in southeastern Bahia, Brazil.

This study was conducted to link soil and litter microbial biomass and activity with soil and litter quality in the surface layer for different pure and mixed stands of native tree species in southeastern Bahia, Brazil. The purpose of the study was to see how strongly the differences among species and stands affect the microbiological attributes of the soil and to identify how microbial processes can be influenced by soil and litter quality. Soil and litter samples were collected from six pure and mixed stands of six hardwood species (Peltogyne angustifolia, Centrolobium robustum, Arapatiella psilophylla, Sclerolobium chrysophyllum, Cordia trichotoma, Macrolobium latifolium) native to the southeastern region of Bahia, Brazil. In plantations of native tree species in humid tropical regions, the immobilization efficiency of C and N by soil microbial biomass was strongly related to the chemical quality of the litter and to the organic matter quality of the soil. According to the variables analyzed, the mixed stand was similar to the natural forest and dissimilar to the pure stands. Litter microbial biomass represented a greater sink of C and N than soil microbial biomass and is an important contributor of resources to tropical soils having low C and N availability.

C and N content in density fractions of whole soil and soil size fraction under cacao agroforestry systems and natural forest in Bahia, Brazil.

Agroforestry systems (AFSs) have an important role in capturing above and below ground soil carbon and play a dominant role in mitigation of atmospheric CO(2). Attempts has been made here to identify soil organic matter fractions in the cacao-AFSs that have different susceptibility to microbial decomposition and further represent the basis of understanding soil C dynamics. The objective of this study was to characterize the organic matter density fractions and soil size fractions in soils of two types of cacao agroforestry systems and to compare with an adjacent natural forest in Bahia, Brazil. The land-use systems studied were: (1) a 30-year-old stand of natural forest with cacao (cacao cabruca), (2) a 30-year-old stand of cacao with Erythrina glauca as shade trees (cacao + erythrina), and (3) an adjacent natural forest without cacao. Soil samples were collected from 0-10 cm depth layer in reddish-yellow Oxisols. Soil samples was separated by wet sieving into five fraction-size classes (>2000 µm, 1000-2000 µm, 250-1000 µm, 53-250 µm, and <53 µm). C and N accumulated in to the light (free- and intra-aggregate density fractions) and heavy fractions of whole soil and soil size fraction were determined. Soil size fraction obtained in cacao AFS soils consisted mainly (65 %) of mega-aggregates (>2000 µm) mixed with macroaggregates (32-34%), and microaggregates (1-1.3%). Soil organic carbon (SOC) and total N content increased with increasing soil size fraction in all land-use systems. Organic C-to-total N ratio was higher in the macroaggregate than in the microaggregate. In general, in natural forest and cacao cabruca the contribution of C and N in the light and heavy fractions was similar. However, in cacao + erythrina the heavy fraction was the most common and contributed 67% of C and 63% of N. Finding of this study shows that the majority of C and N in all three systems studied are found in macroaggregates, particularly in the 250-1000 µm size aggregate class. The heavy fraction was the most common organic matter fraction in these soils. Thus, in mature cacao AFS on highly weathered soils the main mechanisms of C stabilization could be the physical protection within macroaggregate structures thereby minimizing the impact of conversion of forest to cacao AFS.